##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccI AscI AseI
AsnI Asp718I AspI BalI BbeI
BciVI BclI BsiWI BsmI Bsp1407I
BspDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EcoRI EcoRV FbaI FseI HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PmeI
PshAI PshBI PvuI RsrII SacII
SalI SapI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 3433 TCCGGA T^CCGGA
AclI 2492 AACGTT AA^CGTT
AcsI 3943 AAATTC [GA]^AATT[CT]
AflII 3175 CTTAAG C^TTAAG
AgeI 2086 ACCGGT A^CCGGT
AhdI 354 GACGAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2994 GTGCAC G^TGCAC
AocI 1496 CCTGAGG CC^T[ACGT]AGG
Aor51HI 4089 AGCGCT AGC^GCT
ApaLI 2994 GTGCAC G^TGCAC
ApoI 3943 AAATTC [GA]^AATT[CT]
Asp700I 2132 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 354 GACGAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 666 TGCGCA TGC^GCA
AvrII 3386 CCTAGG C^CTAGG
BamHI 823 GGATCC G^GATCC
BbrPI 3710 CACGTG CAC^GTG
BfrI 3175 CTTAAG C^TTAAG
BglII 1681 AGATCT A^GATCT
BlnI 3386 CCTAGG C^CTAGG
BsaBI 1697 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3433 TCCGGA T^CCGGA
BspEI 3433 TCCGGA T^CCGGA
BsrFI 2086 ACCGGT [GA]^CCGG[CT]
BstAPI 560 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1859 GGTGACC G^GT[ACGT]ACC
BstPI 1859 GGTGACC G^GT[ACGT]ACC
Bsu36I 1496 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2086 ACCGGT [GA]^CCGG[CT]
DraI 4144 TTTAAA TTT^AAA
DraIII 1244 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2160 CGGCCG C^GGCCG
Eam1105I 354 GACGAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2160 CGGCCG C^GGCCG
Eco47III 4089 AGCGCT AGC^GCT
Eco52I 2160 CGGCCG C^GGCCG
Eco81I 1496 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1859 GGTGACC G^GT[ACGT]ACC
EcoT22I 21 ATGCAT ATGCA^T
FspI 666 TGCGCA TGC^GCA
HincII 1650 GTCAAC GT[CT]^[GA]AC
HindII 1650 GTCAAC GT[CT]^[GA]AC
MamI 1697 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3433 TCCGGA T^CCGGA
NsiI 21 ATGCAT ATGCA^T
PinAI 2086 ACCGGT A^CCGGT
PmaCI 3710 CACGTG CAC^GTG
PmlI 3710 CACGTG CAC^GTG
Psp1406I 2492 AACGTT AA^CGTT
ScaI 925 AGTACT AGT^ACT
XmnI 2132 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 993 GACGCC G[GA]^CG[CT]C
4027 GGCGTC
BbvCI 1325 CCTCAGC CCTCAGC(-5/-2)
939 GCTGAGG
BsaHI 993 GACGCC G[GA]^CG[CT]C
4027 GGCGTC
BsiEI 584 CGACCG CG[GA][CT]^CG
2160 CGGCCG
BsmBI 813 GAGACG CGTCTC(1/5)
1212 GAGACG
BspHI 1159 TCATGA T^CATGA
3449 TCATGA
BspLU11I 2068 ACATGT A^CATGT
3487 ACATGT
BsrBI 3222 CCGCTC CCGCTC(-3/-3)
1749 GAGCGG
EaeI 2160 CGGCCG [CT]^GGCC[GA]
2745 CGGCCA
Hin1I 993 GACGCC G[GA]^CG[CT]C
4027 GGCGTC
RcaI 1159 TCATGA T^CATGA
3449 TCATGA
SbfI 1304 CCTGCAGG CCTGCA^GG
3183 CCTGCAGG
SexAI 547 ACCTGGT A^CC[AT]GGT
3573 ACCAGGT
SmaI 2178 CCCGGG CCC^GGG
3802 CCCGGG
Sse8387I 1304 CCTGCAGG CCTGCA^GG
3183 CCTGCAGG
XmaCI 2178 CCCGGG C^CCGGG
3802 CCCGGG
XmaI 2178 CCCGGG C^CCGGG
3802 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PaeR7I PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII