##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
BfrI        BglII       BsaBI       BsiWI       BspDI       
BsrDI       BssHII      Bst1107I    BstAPI      BstZ17I     
ClaI        CpoI        Eco47III    EcoT22I     FseI        
HpaI        KpnI        MamI        MfeI        MluI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        PacI        PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SalI        SfiI        
SgrAI       SnaBI       SpeI        SwaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII        1842  GACGTC               GACGT^C
AccII         201  CGCG                 CG^CG
AhdI         1065  GACGAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      2525  GAAGGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1065  GACGAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          168  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         469  TGCGCA               TGC^GCA
AvrII         569  CCTAGG               C^CTAGG
BamHI        2643  GGATCC               G^GATCC
BbrPI        3423  CACGTG               CAC^GTG
BcgI           19  GCATCATCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI          569  CCTAGG               C^CTAGG
BlpI         1810  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1810  GCTCAGC              GC^T[ACGT]AGC
BsmI         2144  GAATGC               GAATGC(1/-1)
BsrFI        1649  GCCGGC               [GA]^CCGG[CT]
BstUI         201  CGCG                 CG^CG
CelII        1810  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1649  GCCGGC               [GA]^CCGG[CT]
EagI         1651  CGGCCG               C^GGCCG
Eam1105I     1065  GACGAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1651  CGGCCG               C^GGCCG
Eco52I       1651  CGGCCG               C^GGCCG
EcoRI        3502  GAATTC               G^AATTC
FspI          469  TGCGCA               TGC^GCA
HgaI         1924  GCGTC                GACGC(5/10)
HindIII      3243  AAGCTT               A^AGCTT
KspI          200  CCGCGG               CCGC^GG
MvnI          201  CGCG                 CG^CG
NaeI         1649  GCCGGC               GCC^GGC
NgoMIV       1649  GCCGGC               G^CCGGC
PaeR7I        153  CTCGAG               C^TCGAG
PflFI         168  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3423  CACGTG               CAC^GTG
PmlI         3423  CACGTG               CAC^GTG
SacII         200  CCGCGG               CCGC^GG
SbfI         1673  CCTGCAGG             CCTGCA^GG
SexAI         169  ACCTGGT              A^CC[AT]GGT
SphI         3395  GCATGC               GCATG^C
Sse8387I     1673  CCTGCAGG             CCTGCA^GG
Tth111I       168  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         4084  CCACCAACTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          153  CTCGAG               C^TCGAG
XmnI         2525  GAAGGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccIII        244  TCCGGA               T^CCGGA
             2633  TCCGGA              
BalI         1243  TGGCCA               TGG^CCA
             3788  TGGCCA              
BbeI         1728  GGCGCC               GGCGC^C
             3515  GGCGCC              
BbvCI        2767  GCTGAGG              CCTCAGC(-5/-2)
             2906  GCTGAGG             
BciVI        2242  GGATAC               GTATCC(6/5)
             3736  GTATCC              
BclI         2795  TGATCA               T^GATCA
             4123  TGATCA              
BsaAI        3423  CACGTG               [CT]AC^GT[GA]
             4274  CACGTA              
BsaWI         244  TCCGGA               [AT]^CCGG[AT]
             2633  TCCGGA              
BseAI         244  TCCGGA               T^CCGGA
             2633  TCCGGA              
BsiEI        1651  CGGCCG               CG[GA][CT]^CG
             1775  CGACCG              
BsmBI        1844  CGTCTC               CGTCTC(1/5)
             1722  GAGACG              
BspEI         244  TCCGGA               T^CCGGA
             2633  TCCGGA              
BspHI         955  TCATGA               T^CATGA
              979  TCATGA              
BspLU11I     1366  ACATGT               A^CATGT
             3278  ACATGT              
BsrBI        1486  CCGCTC               CCGCTC(-3/-3)
             1808  CCGCTC              
BstBI        1059  TTCGAA               TT^CGAA
             3232  TTCGAA              
DraI         2439  TTTAAA               TTT^AAA
             2484  TTTAAA              
DraIII       1167  CACCCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3612  CACTGGGTG           
EcoRV         951  GATATC               GAT^ATC
             1535  GATATC              
FbaI         2795  TGATCA               T^GATCA
             4123  TGATCA              
KasI         1728  GGCGCC               G^GCGCC
             3515  GGCGCC              
MluNI        1243  TGGCCA               TGG^CCA
             3788  TGGCCA              
MroI          244  TCCGGA               T^CCGGA
             2633  TCCGGA              
MscI         1243  TGGCCA               TGG^CCA
             3788  TGGCCA              
NarI         1728  GGCGCC               GG^CGCC
             3515  GGCGCC              
NspV         1059  TTCGAA               TT^CGAA
             3232  TTCGAA              
PshAI        1760  GACCTCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3588  GACCAAGGTC          
RcaI          955  TCATGA               T^CATGA
              979  TCATGA              
SapI          335  GCTCTTC              GCTCTTC(1/4)
             2690  GCTCTTC             
ScaI         1612  AGTACT               AGT^ACT
             3472  AGTACT              
SfoI         1728  GGCGCC               GGC^GCC
             3515  GGCGCC              
SfuI         1059  TTCGAA               TT^CGAA
             3232  TTCGAA              
StuI         2998  AGGCCT               AGG^CCT
             3664  AGGCCT              
XbaI          608  TCTAGA               T^CTAGA
             2863  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       AocI        
ApaI        ApaLI       ApoI        AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspMI       
BsrGI       BsrI        BssKI       BssSI       BstEII      
BstF5I      BstNI       BstPI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DrdI        DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        SspBI       SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmaCI       
XmaI