##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AhdI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BciVI
BclI BfrI BglII BlnI BsaBI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SexAI SfuI
SnaBI SpeI SspBI SspI SwaI
Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 4228 TCCGGA T^CCGGA
AflIII 4695 ACGTGT A^C[GA][CT]GT
AgeI 2666 ACCGGT A^CCGGT
AscI 1024 GGCGCGCC GG^CGCGCC
AviII 1160 TGCGCA TGC^GCA
BalI 2492 TGGCCA TGG^CCA
BamHI 3169 GGATCC G^GATCC
BbsI 1874 GTCTTC GAAGAC(2/6)
BcgI 4379 CGAGACCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1874 GTCTTC GAAGAC(2/6)
BseAI 4228 TCCGGA T^CCGGA
BspEI 4228 TCCGGA T^CCGGA
BspHI 1176 TCATGA T^CATGA
BsrDI 3247 GCAATG GCAATG(2/0)
BstAPI 796 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1991 GGTCACC G^GT[ACGT]ACC
BstPI 1991 GGTCACC G^GT[ACGT]ACC
DraIII 2330 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1991 GGTCACC G^GT[ACGT]ACC
FspI 1160 TGCGCA TGC^GCA
MluNI 2492 TGGCCA TGG^CCA
MroI 4228 TCCGGA T^CCGGA
MscI 2492 TGGCCA TGG^CCA
PaeR7I 553 CTCGAG C^TCGAG
PinAI 2666 ACCGGT A^CCGGT
RcaI 1176 TCATGA T^CATGA
SapI 910 GCTCTTC GCTCTTC(1/4)
ScaI 1706 AGTACT AGT^ACT
SgrAI 24 CGCCGGCG C[GA]^CCGG[CT]G
Tsp509I 4702 AATT ^AATT
XbaI 2929 TCTAGA T^CTAGA
XhoI 553 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 357 GTCTAC GT^[AC][GT]AC
545 GTAGAC
Aor51HI 1145 AGCGCT AGC^GCT
2483 AGCGCT
BbvCI 3182 CCTCAGC CCTCAGC(-5/-2)
4336 GCTGAGG
BssSI 1865 CACGAG CACGAG(-5/-1)
4490 CTCGTG
BstYI 71 AGATCC [GA]^GATC[CT]
3169 GGATCC
DrdI 2737 GACTCGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3503 GACCCTGAGGTC
Eco47III 1145 AGCGCT AGC^GCT
2483 AGCGCT
HincII 220 GTCAAC GT[CT]^[GA]AC
3751 GTCAAC
HindII 220 GTCAAC GT[CT]^[GA]AC
3751 GTCAAC
MflI 71 AGATCC [GA]^GATC[CT]
3169 GGATCC
SbfI 793 CCTGCAGG CCTGCA^GG
2901 CCTGCAGG
SfiI 1084 GGCCGCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3155 GGCCGTGAGGGCC
SmaI 3466 CCCGGG CCC^GGG
4350 CCCGGG
SmlI 553 CTCGAG C^T[CT][GA]AG
3993 CTCAAG
Sse8387I 793 CCTGCAGG CCTGCA^GG
2901 CCTGCAGG
StuI 1071 AGGCCT AGG^CCT
4022 AGGCCT
TfiI 108 GATTC G^A[AT]TC
3555 GATTC
XhoII 71 AGATCC [GA]^GATC[CT]
3169 GGATCC
XmaCI 3466 CCCGGG C^CCGGG
4350 CCCGGG
XmaI 3466 CCCGGG C^CCGGG
4350 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BanI
BanII BbeI BbrPI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SphI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI