##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AflIII Aor51HI AscI AseI
AsnI Asp718I AviII BamHI BglII
BsiWI BsmBI BsmI BspLU11I BssHII
BstBI CpoI Eco47III EcoRV EcoT22I
FseI FspI HincII HindII HpaI
KpnI KspI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshAI PshBI PvuI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 2370 GACGTC GACGT^C
AccII 2661 CGCG CG^CG
AccIII 1577 TCCGGA T^CCGGA
AclI 329 AACGTT AA^CGTT
AgeI 1571 ACCGGT A^CCGGT
AhdI 130 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1510 GTGCAC G^TGCAC
ApaLI 1510 GTGCAC G^TGCAC
AspEI 130 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1327 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3444 CCTAGG C^CTAGG
BbeI 62 GGCGCC GGCGC^C
BbrPI 3302 CACGTG CAC^GTG
BbvCI 1845 CCTCAGC CCTCAGC(-5/-2)
BglI 1741 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3444 CCTAGG C^CTAGG
BlpI 2265 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2265 GCTTAGC GC^T[ACGT]AGC
BsaAI 3302 CACGTG [CT]AC^GT[GA]
BsaBI 1075 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1577 TCCGGA T^CCGGA
BsiEI 95 CGGCCG CG[GA][CT]^CG
Bsp1407I 905 TGTACA T^GTACA
BspDI 1246 ATCGAT AT^CGAT
BspEI 1577 TCCGGA T^CCGGA
BsrGI 905 TGTACA T^GTACA
BssSI 2816 CTCGTG CACGAG(-5/-1)
Bst1107I 1953 GTATAC GTA^TAC
BstUI 2661 CGCG CG^CG
BstZ17I 1953 GTATAC GTA^TAC
CelII 2265 GCTTAGC GC^T[ACGT]AGC
ClaI 1246 ATCGAT AT^CGAT
EagI 95 CGGCCG C^GGCCG
Eam1105I 130 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 95 CGGCCG C^GGCCG
Eco52I 95 CGGCCG C^GGCCG
EcoRI 3436 GAATTC G^AATTC
HindIII 1400 AAGCTT A^AGCTT
KasI 62 GGCGCC G^GCGCC
MamI 1075 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1577 TCCGGA T^CCGGA
MvnI 2661 CGCG CG^CG
NarI 62 GGCGCC GG^CGCC
NcoI 3292 CCATGG C^CATGG
NdeI 3172 CATATG CA^TATG
NspI 2166 GCATGT [GA]CATG^[CT]
PflFI 1327 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1571 ACCGGT A^CCGGT
PmaCI 3302 CACGTG CAC^GTG
PmlI 3302 CACGTG CAC^GTG
Psp1406I 329 AACGTT AA^CGTT
SapI 3361 GCTCTTC GCTCTTC(1/4)
ScaI 2111 AGTACT AGT^ACT
SexAI 789 ACCAGGT A^CC[AT]GGT
SfoI 62 GGCGCC GGC^GCC
SspBI 905 TGTACA T^GTACA
SspI 3921 AATATT AAT^ATT
Tth111I 1327 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4262 TCTAGA T^CTAGA
XcmI 3298 CCACCACGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1006 GTAGAC GT^[AC][GT]AC
1953 GTATAC
AflII 1306 CTTAAG C^TTAAG
3479 CTTAAG
BcgI 3197 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3711 CGAAGCATCTGC
BciVI 2139 GGATAC GTATCC(6/5)
113 GTATCC
BclI 1074 TGATCA T^GATCA
1533 TGATCA
BfrI 1306 CTTAAG C^TTAAG
3479 CTTAAG
BsrBI 721 GAGCGG CCGCTC(-3/-3)
2464 GAGCGG
BsrDI 2574 CATTGC GCAATG(2/0)
3783 CATTGC
BstAPI 1961 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2044 GCAAAATATGC
BstEII 2869 GGTGACC G^GT[ACGT]ACC
3521 GGTCACC
BstPI 2869 GGTGACC G^GT[ACGT]ACC
3521 GGTCACC
BstXI 3286 CCAGGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3301 CCACGTGGCTGG
EcoNI 762 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2324 CCTACTGGAGG
EcoO65I 2869 GGTGACC G^GT[ACGT]ACC
3521 GGTCACC
FbaI 1074 TGATCA T^GATCA
1533 TGATCA
HgaI 80 GACGC GACGC(5/10)
2659 GACGC
SacI 688 GAGCTC GAGCT^C
1426 GAGCTC
SmaI 490 CCCGGG CCC^GGG
1722 CCCGGG
StuI 1964 AGGCCT AGG^CCT
2668 AGGCCT
XmaCI 490 CCCGGG C^CCGGG
1722 CCCGGG
XmaI 490 CCCGGG C^CCGGG
1722 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DrdI DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI