##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
AseI AsnI AspI AviII AvrII
BbeI BcgI BlnI BlpI Bpu1102I
BsaI BspDI BspLU11I BspMI BsrBI
Bst1107I BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DrdI Eco47III
EcoNI EcoO65I EcoRV FseI FspI
HpaI KasI KspI MfeI MunI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 2535 GTCTAC GT^[AC][GT]AC
AccIII 942 TCCGGA T^CCGGA
AcyI 2579 GGCGTC G[GA]^CG[CT]C
AflIII 1167 ACGCGT A^C[GA][CT]GT
AhdI 2527 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2219 GGGCCC GGGCC^C
AspEI 2527 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3007 CCCGAG C^[CT]CG[GA]G
BamHI 1731 GGATCC G^GATCC
BbvCI 2737 GCTGAGG CCTCAGC(-5/-2)
BciVI 3398 GTATCC GTATCC(6/5)
BclI 1006 TGATCA T^GATCA
BglI 2212 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3241 AGATCT A^GATCT
BsaBI 1312 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2579 GGCGTC G[GA]^CG[CT]C
BseAI 942 TCCGGA T^CCGGA
BsiWI 1668 CGTACG C^GTACG
BsmBI 1495 GAGACG CGTCTC(1/5)
BsoBI 3007 CCCGAG C^[CT]CG[GA]G
Bsp1407I 324 TGTACA T^GTACA
BspEI 942 TCCGGA T^CCGGA
BsrDI 2590 GCAATG GCAATG(2/0)
BsrFI 557 GCCGGT [GA]^CCGG[CT]
BsrGI 324 TGTACA T^GTACA
BssHII 1871 GCGCGC G^CGCGC
BstBI 596 TTCGAA TT^CGAA
Cfr10I 557 GCCGGT [GA]^CCGG[CT]
EagI 1027 CGGCCG C^GGCCG
Eam1105I 2527 GACCCTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2707 GAAGAG CTCTTC(1/4)
EclXI 1027 CGGCCG C^GGCCG
Eco52I 1027 CGGCCG C^GGCCG
EcoRI 3606 GAATTC G^AATTC
FbaI 1006 TGATCA T^GATCA
Hin1I 2579 GGCGTC G[GA]^CG[CT]C
HincII 1624 GTCAAC GT[CT]^[GA]AC
HindII 1624 GTCAAC GT[CT]^[GA]AC
Ksp632I 2707 GAAGAG CTCTTC(1/4)
MamI 1312 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1167 ACGCGT A^CGCGT
MroI 942 TCCGGA T^CCGGA
NcoI 2898 CCATGG C^CATGG
NdeI 3700 CATATG CA^TATG
NspV 596 TTCGAA TT^CGAA
PshAI 917 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1944 GAGCTC GAGCT^C
SfuI 596 TTCGAA TT^CGAA
SspBI 324 TGTACA T^GTACA
SspI 2924 AATATT AAT^ATT
TaqI 597 TCGA T^CGA
TthHB8I 597 TCGA T^CGA
XbaI 2596 TCTAGA T^CTAGA
XcmI 3273 CCACTTTGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 3286 GGTACC G^GTACC
3506 GGTACC
AflII 1379 CTTAAG C^TTAAG
3134 CTTAAG
Alw44I 1171 GTGCAC G^TGCAC
1721 GTGCAC
AocI 874 CCTTAGG CC^T[ACGT]AGG
1798 CCTCAGG
ApaLI 1171 GTGCAC G^TGCAC
1721 GTGCAC
Asp718I 3286 GGTACC G^GTACC
3506 GGTACC
BalI 2469 TGGCCA TGG^CCA
3530 TGGCCA
BfrI 1379 CTTAAG C^TTAAG
3134 CTTAAG
BsiEI 1027 CGGCCG CG[GA][CT]^CG
3692 CGACCG
BstAPI 837 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1173 GCACGCAGTGC
Bsu36I 874 CCTTAGG CC^T[ACGT]AGG
1798 CCTCAGG
DraI 2850 TTTAAA TTT^AAA
3473 TTTAAA
DraII 656 AGGCCCT [GA]G^G[ACGT]CC[CT]
1076 AGGCCCT
Eco81I 874 CCTTAGG CC^T[ACGT]AGG
1798 CCTCAGG
EcoO109I 656 AGGCCCT [GA]G^G[ACGT]CC[CT]
1076 AGGCCCT
EcoT14I 2530 CCTTGG C^C[AT][AT]GG
2898 CCATGG
EcoT22I 743 ATGCAT ATGCA^T
936 ATGCAT
HaeII 443 GGCGCT [GA]GCGC^[CT]
455 GGCGCT
HindIII 1104 AAGCTT A^AGCTT
1440 AAGCTT
KpnI 3286 GGTACC GGTAC^C
3506 GGTACC
MluNI 2469 TGGCCA TGG^CCA
3530 TGGCCA
MscI 2469 TGGCCA TGG^CCA
3530 TGGCCA
NsiI 743 ATGCAT ATGCA^T
936 ATGCAT
PvuII 2385 CAGCTG CAG^CTG
3501 CAGCTG
StyI 2530 CCTTGG C^C[AT][AT]GG
2898 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbrPI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraIII DsaI EaeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII XmnI