##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
AhdI AscI AspEI BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BpuAI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I ClaI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoRV FbaI FseI HincII
HindII HpaI KasI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI SacI
SalI SapI SbfI ScaI SfiI
SfoI SfuI SnaBI SphI Sse8387I
SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 337 GACGTC GACGT^C
AccI 2686 GTAGAC GT^[AC][GT]AC
AcyI 337 GACGTC G[GA]^CG[CT]C
Alw44I 579 GTGCAC G^TGCAC
Aor51HI 2849 AGCGCT AGC^GCT
ApaLI 579 GTGCAC G^TGCAC
AseI 3606 ATTAAT AT^TAAT
AsnI 3606 ATTAAT AT^TAAT
AspI 204 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 264 TGCGCA TGC^GCA
AvrII 1513 CCTAGG C^CTAGG
BglI 1999 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1513 CCTAGG C^CTAGG
BlpI 1693 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1693 GCTGAGC GC^T[ACGT]AGC
BsaHI 337 GACGTC G[GA]^CG[CT]C
BsaI 310 GGTCTC GGTCTC(1/5)
BsaWI 1475 ACCGGA [AT]^CCGG[AT]
BsmI 3084 GAATGC GAATGC(1/-1)
Bsp1407I 2648 TGTACA T^GTACA
BsrBI 844 CCGCTC CCGCTC(-3/-3)
BsrDI 3013 GCAATG GCAATG(2/0)
BsrGI 2648 TGTACA T^GTACA
BstAPI 1704 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1518 GGTAACC G^GT[ACGT]ACC
BstPI 1518 GGTAACC G^GT[ACGT]ACC
CelII 1693 GCTGAGC GC^T[ACGT]AGC
CpoI 1401 CGGACCG CG^G[AT]CCG
DraIII 815 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1924 GAAGAG CTCTTC(1/4)
Eco47III 2849 AGCGCT AGC^GCT
EcoO65I 1518 GGTAACC G^GT[ACGT]ACC
EcoT22I 3086 ATGCAT ATGCA^T
FspI 264 TGCGCA TGC^GCA
HgaI 591 GACGC GACGC(5/10)
Hin1I 337 GACGTC G[GA]^CG[CT]C
Ksp632I 1924 GAAGAG CTCTTC(1/4)
KspI 1068 CCGCGG CCGC^GG
NaeI 987 GCCGGC GCC^GGC
NgoMIV 987 GCCGGC G^CCGGC
NheI 101 GCTAGC G^CTAGC
NsiI 3086 ATGCAT ATGCA^T
NspI 2820 GCATGT [GA]CATG^[CT]
PflFI 204 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2084 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3606 ATTAAT AT^TAAT
PvuII 276 CAGCTG CAG^CTG
RsrII 1401 CGGACCG CG^G[AT]CCG
SacII 1068 CCGCGG CCGC^GG
SexAI 816 ACCTGGT A^CC[AT]GGT
SgrAI 191 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 1643 CCCGGG CCC^GGG
SpeI 2610 ACTAGT A^CTAGT
SspBI 2648 TGTACA T^GTACA
SspI 1977 AATATT AAT^ATT
Tsp45I 589 GTGAC ^GT[GC]AC
Tth111I 204 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3371 TCTAGA T^CTAGA
XmaCI 1643 CCCGGG C^CCGGG
XmaI 1643 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 681 GGTACC G^GTACC
2367 GGTACC
AocI 1555 CCTAAGG CC^T[ACGT]AGG
1864 CCTGAGG
Asp700I 3236 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3346 GAAATCTTTC
Asp718I 681 GGTACC G^GTACC
2367 GGTACC
AvaI 1440 CCCGAG C^[CT]CG[GA]G
1643 CCCGGG
BbvCI 424 GCTGAGG CCTCAGC(-5/-2)
2305 GCTGAGG
BseRI 1219 GAGGAG GAGGAG(10/8)
2677 GAGGAG
BsoBI 1440 CCCGAG C^[CT]CG[GA]G
1643 CCCGGG
BspMI 2830 ACCTGC ACCTGC(4/8)
2724 GCAGGT
BsrFI 192 GCCGGT [GA]^CCGG[CT]
987 GCCGGC
BstF5I 2372 CATCC GGATG(2/0)
2033 GGATG
Bsu36I 1555 CCTAAGG CC^T[ACGT]AGG
1864 CCTGAGG
Cfr10I 192 GCCGGT [GA]^CCGG[CT]
987 GCCGGC
Eco81I 1555 CCTAAGG CC^T[ACGT]AGG
1864 CCTGAGG
FokI 2372 CATCC GGATG(9/13)
2033 GGATG
HaeII 472 AGCGCC [GA]GCGC^[CT]
2849 AGCGCT
HindIII 2733 AAGCTT A^AGCTT
3419 AAGCTT
KpnI 681 GGTACC GGTAC^C
2367 GGTACC
MaeII 338 ACGT A^CGT
1688 ACGT
SmlI 468 CTCAAG C^T[CT][GA]AG
2841 CTTGAG
TaqI 1153 TCGA T^CGA
1583 TCGA
TthHB8I 1153 TCGA T^CGA
1583 TCGA
XcmI 1523 CCAAGGCCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1616 CCATTAGTATTATGG
XmnI 3236 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3346 GAAATCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII