##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BcgI        BclI        BfrI        
BglII       BlnI        BsaWI       BseAI       BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BsrFI       BsrGI       BssHII      Bst1107I    BstZ17I     
Cfr10I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HpaI        KasI        KspI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       2667  GGTACC               G^GTACC
AflIII       2376  ACATGT               A^C[GA][CT]GT
Aor51HI      1459  AGCGCT               AGC^GCT
ApaI         2060  GGGCCC               GGGCC^C
Asp700I      2752  GAAACTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2667  GGTACC               G^GTACC
BamHI         789  GGATCC               G^GATCC
BcnI         1352  CCCGG                CC^[GC]GG
BsaAI        2336  CACGTA               [CT]AC^GT[GA]
BsaBI         598  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2779  GGTCTC               GGTCTC(1/5)
BsmBI        1156  CGTCTC               CGTCTC(1/5)
BspHI         840  TCATGA               T^CATGA
BspLU11I     2376  ACATGT               A^CATGT
BsrBI        2076  GAGCGG               CCGCTC(-3/-3)
BsrDI         749  GCAATG               GCAATG(2/0)
BssSI        2330  CACGAG               CACGAG(-5/-1)
BstAPI       1538  GCATCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         616  TTCGAA               TT^CGAA
BstEII       1276  GGTGACC              G^GT[ACGT]ACC
BstPI        1276  GGTGACC              G^GT[ACGT]ACC
BstXI         976  CCAGCACACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII       2175  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1459  AGCGCT               AGC^GCT
EcoO65I      1276  GGTGACC              G^GT[ACGT]ACC
EcoT14I      1272  CCTTGG               C^C[AT][AT]GG
HapII        1353  CCGG                 C^CGG
HindIII       350  AAGCTT               A^AGCTT
HpaII        1353  CCGG                 C^CGG
KpnI         2667  GGTACC               GGTAC^C
MamI          598  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI         1353  CCGG                 C^CGG
NciI         1352  CCCGG                CC^[GC]GG
NspV          616  TTCGAA               TT^CGAA
PaeR7I       1072  CTCGAG               C^TCGAG
PflMI          37  CCAGATTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII        2144  CAGCTG               CAG^CTG
RcaI          840  TCATGA               T^CATGA
SfuI          616  TTCGAA               TT^CGAA
SphI         1674  GCATGC               GCATG^C
StyI         1272  CCTTGG               C^C[AT][AT]GG
Van91I         37  CCAGATTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1072  CTCGAG               C^TCGAG
XmnI         2752  GAAACTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         1947  GTCTAC               GT^[AC][GT]AC
             1970  GTCTAC              
AcyI         1178  GGCGTC               G[GA]^CG[CT]C
             2017  GACGCC              
BalI         1789  TGGCCA               TGG^CCA
             2837  TGGCCA              
BciVI         441  GGATAC               GTATCC(6/5)
             2818  GTATCC              
BglI         1791  GCCAGCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2601  GCCCAGTGGGC         
BsaHI        1178  GGCGTC               G[GA]^CG[CT]C
             2017  GACGCC              
BseRI         270  CTCCTC               GAGGAG(10/8)
             1069  CTCCTC              
BspMI        2239  ACCTGC               ACCTGC(4/8)
             1898  GCAGGT              
BstYI         459  AGATCC               [GA]^GATC[CT]
              789  GGATCC              
DsaI         1140  CCACGG               C^C[GA][CT]GG
             1525  CCGTGG              
EarI         1111  CTCTTC               CTCTTC(1/4)
             2281  GAAGAG              
FauI         1235  CCCGC                CCCGC(4/6)
             1550  CCCGC               
HaeII        1106  AGCGCC               [GA]GCGC^[CT]
             1459  AGCGCT              
HgaI         2017  GACGC                GACGC(5/10)
             1179  GCGTC               
Hin1I        1178  GGCGTC               G[GA]^CG[CT]C
             2017  GACGCC              
Ksp632I      1111  CTCTTC               CTCTTC(1/4)
             2281  GAAGAG              
MaeII        1079  ACGT                 A^CGT
             2337  ACGT                
MflI          459  AGATCC               [GA]^GATC[CT]
              789  GGATCC              
MluNI        1789  TGGCCA               TGG^CCA
             2837  TGGCCA              
MscI         1789  TGGCCA               TGG^CCA
             2837  TGGCCA              
MseI          509  TTAA                 T^TAA
              626  TTAA                
MspA1I       2144  CAGCTG               C[AC]G^C[GT]G
             2813  CAGCGG              
PshAI        1150  GACCTCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2375  GACATGTGTC          
SacI          925  GAGCTC               GAGCT^C
             1394  GAGCTC              
Tru9I         509  TTAA                 T^TAA
              626  TTAA                
XcmI         1771  CCAGCAGGCCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2055  CCACTGGGCCCCTGG     
XhoII         459  AGATCC               [GA]^GATC[CT]
              789  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvCI       BbvI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Bsu36I      Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRII      Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HinfI       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MslI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I