##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AviII BanI
BbeI BsaBI BsaHI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstZ17I ClaI CpoI
DraI DraIII Eam1105I Eco47III EcoRV
FseI FspI Hin1I HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmeI PshBI RcaI RsrII SacII
SalI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SspI StuI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AclI 2694 AACGTT AA^CGTT
AflII 126 CTTAAG C^TTAAG
AflIII 3062 ACACGT A^C[GA][CT]GT
AocI 3409 CCTGAGG CC^T[ACGT]AGG
ApaI 3961 GGGCCC GGGCC^C
AvaI 2175 CTCGGG C^[CT]CG[GA]G
AvrII 2760 CCTAGG C^CTAGG
BbrPI 317 CACGTG CAC^GTG
BciVI 3382 GTATCC GTATCC(6/5)
BfrI 126 CTTAAG C^TTAAG
BglII 491 AGATCT A^GATCT
BlnI 2760 CCTAGG C^CTAGG
BlpI 720 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 720 GCTGAGC GC^T[ACGT]AGC
BsoBI 2175 CTCGGG C^[CT]CG[GA]G
Bsp1407I 4233 TGTACA T^GTACA
BsrDI 3314 GCAATG GCAATG(2/0)
BsrFI 3269 ACCGGC [GA]^CCGG[CT]
BsrGI 4233 TGTACA T^GTACA
BstBI 172 TTCGAA TT^CGAA
BstEII 3765 GGTAACC G^GT[ACGT]ACC
BstPI 3765 GGTAACC G^GT[ACGT]ACC
Bsu36I 3409 CCTGAGG CC^T[ACGT]AGG
CelII 720 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3269 ACCGGC [GA]^CCGG[CT]
EagI 3790 CGGCCG C^GGCCG
EclXI 3790 CGGCCG C^GGCCG
Eco52I 3790 CGGCCG C^GGCCG
Eco81I 3409 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3765 GGTAACC G^GT[ACGT]ACC
EcoT22I 1957 ATGCAT ATGCA^T
MfeI 3649 CAATTG C^AATTG
MunI 3649 CAATTG C^AATTG
NsiI 1957 ATGCAT ATGCA^T
NspV 172 TTCGAA TT^CGAA
PmaCI 317 CACGTG CAC^GTG
PmlI 317 CACGTG CAC^GTG
PshAI 2527 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2694 AACGTT AA^CGTT
PvuI 1474 CGATCG CGAT^CG
SapI 1915 GAAGAGC GCTCTTC(1/4)
SbfI 2122 CCTGCAGG CCTGCA^GG
SfuI 172 TTCGAA TT^CGAA
SphI 1955 GCATGC GCATG^C
Sse8387I 2122 CCTGCAGG CCTGCA^GG
SspBI 4233 TGTACA T^GTACA
XbaI 330 TCTAGA T^CTAGA
XcmI 180 CCAGCCCATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 389 GTGCAC G^TGCAC
2704 GTGCAC
ApaLI 389 GTGCAC G^TGCAC
2704 GTGCAC
AspI 2503 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4589 GACCCTGTC
BcgI 2794 CGAGGCATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
911 GCAAAGCAATCG
BclI 465 TGATCA T^GATCA
1784 TGATCA
BglI 926 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1334 GCCATGAAGGC
BsaAI 317 CACGTG [CT]AC^GT[GA]
3063 CACGTA
BsiEI 1474 CGATCG CG[GA][CT]^CG
3790 CGGCCG
BsmI 1246 GAATGC GAATGC(1/-1)
1819 GAATGC
BstAPI 3164 GCATTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3639 GCAGCCCCTGC
DrdI 1002 GACACCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3160 GACAGCATTGTC
EcoRI 1691 GAATTC G^AATTC
3901 GAATTC
FbaI 465 TGATCA T^GATCA
1784 TGATCA
HgaI 2988 GACGC GACGC(5/10)
2532 GCGTC
HincII 94 GTTGAC GT[CT]^[GA]AC
3457 GTCAAC
HindII 94 GTTGAC GT[CT]^[GA]AC
3457 GTCAAC
HindIII 301 AAGCTT A^AGCTT
847 AAGCTT
NcoI 872 CCATGG C^CATGG
3086 CCATGG
NspI 113 ACATGC [GA]CATG^[CT]
1955 GCATGC
PflFI 2503 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4589 GACCCTGTC
PvuII 2113 CAGCTG CAG^CTG
2590 CAGCTG
Tth111I 2503 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4589 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BamHI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI