##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AvrII       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BsaBI       BsiWI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BsrGI       
Bst1107I    BstBI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      Eco47III    Eco81I      
EcoRI       EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NdeI        
NheI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        ScaI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        SspBI       SspI        SwaI        
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccIII       1886  TCCGGA               T^CCGGA
AhdI         1403  GACCGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1826  GTGCAC               G^TGCAC
ApaLI        1826  GTGCAC               G^TGCAC
AspEI        1403  GACCGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2060  TGCGCA               TGC^GCA
BamHI        2261  GGATCC               G^GATCC
BseAI        1886  TCCGGA               T^CCGGA
BsmBI         282  CGTCTC               CGTCTC(1/5)
BspEI        1886  TCCGGA               T^CCGGA
BssHII       2449  GCGCGC               G^CGCGC
BstXI         648  CCAAACTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI         2107  GACCGAGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         4264  CGGCCG               C^GGCCG
Eam1105I     1403  GACCGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        4264  CGGCCG               C^GGCCG
Eco52I       4264  CGGCCG               C^GGCCG
EcoRV         155  GATATC               GAT^ATC
FspI         2060  TGCGCA               TGC^GCA
MroI         1886  TCCGGA               T^CCGGA
MseI         3276  TTAA                 T^TAA
NcoI         2493  CCATGG               C^CATGG
NotI         4263  GCGGCCGC             GC^GGCCGC
SbfI          245  CCTGCAGG             CCTGCA^GG
SfiI         1938  GGCCGAACAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         1216  GCATGC               GCATG^C
Sse8387I      245  CCTGCAGG             CCTGCA^GG
TfiI          375  GAATC                G^A[AT]TC
Tru9I        3276  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII       1528  ACGTGT               A^C[GA][CT]GT
             3979  ACGTGT              
AspI         3296  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4201  GACTCCGTC           
BbeI         2580  GGCGCC               GGCGC^C
             3405  GGCGCC              
BbrPI        1527  CACGTG               CAC^GTG
             3748  CACGTG              
BsaAI        1527  CACGTG               [CT]AC^GT[GA]
             3748  CACGTG              
BsaWI        1886  TCCGGA               [AT]^CCGG[AT]
             3384  ACCGGA              
BsiEI        3394  CGACCG               CG[GA][CT]^CG
             4264  CGGCCG              
BsrDI        2839  CATTGC               GCAATG(2/0)
             3797  GCAATG              
BstEII       3837  GGTGACC              G^GT[ACGT]ACC
             3952  GGTAACC             
BstPI        3837  GGTGACC              G^GT[ACGT]ACC
             3952  GGTAACC             
BstYI        2261  GGATCC               [GA]^GATC[CT]
             3478  AGATCC              
DsaI         2493  CCATGG               C^C[GA][CT]GG
             3714  CCACGG              
EcoO65I      3837  GGTGACC              G^GT[ACGT]ACC
             3952  GGTAACC             
KasI         2580  GGCGCC               G^GCGCC
             3405  GGCGCC              
MflI         2261  GGATCC               [GA]^GATC[CT]
             3478  AGATCC              
NarI         2580  GGCGCC               GG^CGCC
             3405  GGCGCC              
PflFI        3296  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4201  GACTCCGTC           
PmaCI        1527  CACGTG               CAC^GTG
             3748  CACGTG              
PmlI         1527  CACGTG               CAC^GTG
             3748  CACGTG              
SapI         1900  GAAGAGC              GCTCTTC(1/4)
             4041  GCTCTTC             
SfoI         2580  GGCGCC               GGC^GCC
             3405  GGCGCC              
Tsp509I       179  AATT                 ^AATT
              501  AATT                
Tth111I      3296  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4201  GACTCCGTC           
XhoII        2261  GGATCC               [GA]^GATC[CT]
             3478  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        StuI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XcmI        
XmaCI       XmaI