##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AvrII BcgI BciVI BclI BfrI
BglII BlnI BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII Eco47III Eco81I
EcoRI EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MunI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 1886 TCCGGA T^CCGGA
AhdI 1403 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1826 GTGCAC G^TGCAC
ApaLI 1826 GTGCAC G^TGCAC
AspEI 1403 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2060 TGCGCA TGC^GCA
BamHI 2261 GGATCC G^GATCC
BseAI 1886 TCCGGA T^CCGGA
BsmBI 282 CGTCTC CGTCTC(1/5)
BspEI 1886 TCCGGA T^CCGGA
BssHII 2449 GCGCGC G^CGCGC
BstXI 648 CCAAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2107 GACCGAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4264 CGGCCG C^GGCCG
Eam1105I 1403 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4264 CGGCCG C^GGCCG
Eco52I 4264 CGGCCG C^GGCCG
EcoRV 155 GATATC GAT^ATC
FspI 2060 TGCGCA TGC^GCA
MroI 1886 TCCGGA T^CCGGA
MseI 3276 TTAA T^TAA
NcoI 2493 CCATGG C^CATGG
NotI 4263 GCGGCCGC GC^GGCCGC
SbfI 245 CCTGCAGG CCTGCA^GG
SfiI 1938 GGCCGAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1216 GCATGC GCATG^C
Sse8387I 245 CCTGCAGG CCTGCA^GG
TfiI 375 GAATC G^A[AT]TC
Tru9I 3276 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 1528 ACGTGT A^C[GA][CT]GT
3979 ACGTGT
AspI 3296 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4201 GACTCCGTC
BbeI 2580 GGCGCC GGCGC^C
3405 GGCGCC
BbrPI 1527 CACGTG CAC^GTG
3748 CACGTG
BsaAI 1527 CACGTG [CT]AC^GT[GA]
3748 CACGTG
BsaWI 1886 TCCGGA [AT]^CCGG[AT]
3384 ACCGGA
BsiEI 3394 CGACCG CG[GA][CT]^CG
4264 CGGCCG
BsrDI 2839 CATTGC GCAATG(2/0)
3797 GCAATG
BstEII 3837 GGTGACC G^GT[ACGT]ACC
3952 GGTAACC
BstPI 3837 GGTGACC G^GT[ACGT]ACC
3952 GGTAACC
BstYI 2261 GGATCC [GA]^GATC[CT]
3478 AGATCC
DsaI 2493 CCATGG C^C[GA][CT]GG
3714 CCACGG
EcoO65I 3837 GGTGACC G^GT[ACGT]ACC
3952 GGTAACC
KasI 2580 GGCGCC G^GCGCC
3405 GGCGCC
MflI 2261 GGATCC [GA]^GATC[CT]
3478 AGATCC
NarI 2580 GGCGCC GG^CGCC
3405 GGCGCC
PflFI 3296 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4201 GACTCCGTC
PmaCI 1527 CACGTG CAC^GTG
3748 CACGTG
PmlI 1527 CACGTG CAC^GTG
3748 CACGTG
SapI 1900 GAAGAGC GCTCTTC(1/4)
4041 GCTCTTC
SfoI 2580 GGCGCC GGC^GCC
3405 GGCGCC
Tsp509I 179 AATT ^AATT
501 AATT
Tth111I 3296 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4201 GACTCCGTC
XhoII 2261 GGATCC [GA]^GATC[CT]
3478 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XmaCI XmaI