##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BanI BbeI BbrPI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsiEI BsiWI BsmBI
BsoBI BspDI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MspA1I MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI PvuII RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 117 CGCG CG^CG
AccIII 34 TCCGGA T^CCGGA
AclI 3608 AACGTT AA^CGTT
AflIII 3594 ACATGT A^C[GA][CT]GT
BanII 1900 GAGCTC G[GA]GC[CT]^C
BbsI 2952 GAAGAC GAAGAC(2/6)
BbvCI 624 CCTCAGC CCTCAGC(-5/-2)
BcgI 69 CGAAGTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3156 AGATCT A^GATCT
BpuAI 2952 GAAGAC GAAGAC(2/6)
BsaBI 1106 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 34 TCCGGA T^CCGGA
BsgI 1043 CTGCAC GTGCAG(16/14)
BspEI 34 TCCGGA T^CCGGA
BspLU11I 3594 ACATGT A^CATGT
BspMI 262 ACCTGC ACCTGC(4/8)
BstAPI 1541 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 117 CGCG CG^CG
BstXI 3977 CCACATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2955 GACAAGTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3922 GAATTC G^AATTC
EcoRV 835 GATATC GAT^ATC
EcoT22I 3839 ATGCAT ATGCA^T
MamI 1106 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 34 TCCGGA T^CCGGA
MvnI 117 CGCG CG^CG
NdeI 3164 CATATG CA^TATG
NsiI 3839 ATGCAT ATGCA^T
PflMI 3541 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1729 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 3608 AACGTT AA^CGTT
SacI 1900 GAGCTC GAGCT^C
SapI 3070 GAAGAGC GCTCTTC(1/4)
SmlI 590 CTCAAG C^T[CT][GA]AG
Van91I 3541 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1649 CCAGGCTCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Asp700I 1387 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2473 GAAGAAATTC
AvaII 982 GGACC G^G[AT]CC
1730 GGTCC
BalI 1589 TGGCCA TGG^CCA
2114 TGGCCA
BamHI 31 GGATCC G^GATCC
1324 GGATCC
BciVI 973 GTATCC GTATCC(6/5)
3289 GTATCC
BclI 2320 TGATCA T^GATCA
3683 TGATCA
BcnI 52 CCGGG CC^[GC]GG
145 CCCGG
BpmI 866 CTGGAG CTGGAG(16/14)
1609 CTGGAG
BsaI 149 GAGACC GGTCTC(1/5)
3375 GGTCTC
BsaWI 34 TCCGGA [AT]^CCGG[AT]
2933 TCCGGT
BseRI 502 GAGGAG GAGGAG(10/8)
1987 GAGGAG
BsmFI 201 GTCCC GGGAC(10/14)
1731 GTCCC
BspHI 1314 TCATGA T^CATGA
2323 TCATGA
DraII 1729 GGGTCCC [GA]G^G[ACGT]CC[CT]
3888 AGGGCCT
EcoO109I 1729 GGGTCCC [GA]G^G[ACGT]CC[CT]
3888 AGGGCCT
EcoT14I 155 CCAAGG C^C[AT][AT]GG
1637 CCTTGG
FbaI 2320 TGATCA T^GATCA
3683 TGATCA
HincII 448 GTTGAC GT[CT]^[GA]AC
751 GTTGAC
HindII 448 GTTGAC GT[CT]^[GA]AC
751 GTTGAC
HphI 712 TCACC GGTGA(8/7)
3141 TCACC
MluNI 1589 TGGCCA TGG^CCA
2114 TGGCCA
MscI 1589 TGGCCA TGG^CCA
2114 TGGCCA
NciI 52 CCGGG CC^[GC]GG
145 CCCGG
RcaI 1314 TCATGA T^CATGA
2323 TCATGA
ScaI 897 AGTACT AGT^ACT
3786 AGTACT
StuI 1634 AGGCCT AGG^CCT
2808 AGGCCT
StyI 155 CCAAGG C^C[AT][AT]GG
1637 CCTTGG
XmnI 1387 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2473 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BbvI BfaI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII