##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BcgI BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsiEI
BsmBI BsoBI BspDI BspEI BspHI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI FseI FspI
HaeII HhaI Hin1I HinP1I KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SpeI Sse8387I StuI SwaI Tth111I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 2957 GTAGAC GT^[AC][GT]AC
AflII 2179 CTTAAG C^TTAAG
Alw44I 276 GTGCAC G^TGCAC
AlwNI 348 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 276 GTGCAC G^TGCAC
AseI 3760 ATTAAT AT^TAAT
AsnI 3760 ATTAAT AT^TAAT
Asp700I 1247 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 805 GGATCC G^GATCC
BbsI 994 GAAGAC GAAGAC(2/6)
BbvCI 654 CCTCAGC CCTCAGC(-5/-2)
BcnI 2104 CCGGG CC^[GC]GG
BfrI 2179 CTTAAG C^TTAAG
BglI 3633 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 386 AGATCT A^GATCT
BpuAI 994 GAAGAC GAAGAC(2/6)
BsaAI 1972 TACGTA [CT]AC^GT[GA]
BsaI 3244 GGTCTC GGTCTC(1/5)
BseRI 119 GAGGAG GAGGAG(10/8)
BsgI 2544 GTGCAG GTGCAG(16/14)
BsiWI 1974 CGTACG C^GTACG
BsiYI 3872 CCAAAGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 3872 CCAAAGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 3245 GTCTC GTCTC(1/5)
Bsp1407I 1478 TGTACA T^GTACA
BsrGI 1478 TGTACA T^GTACA
BssSI 450 CACGAG CACGAG(-5/-1)
DraII 3986 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 131 CCATGG C^C[GA][CT]GG
EarI 116 GAAGAG CTCTTC(1/4)
EcoO109I 3986 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 196 GATATC GAT^ATC
FauI 2279 CCCGC CCCGC(4/6)
HapII 2104 CCGG C^CGG
HgaI 3583 GACGC GACGC(5/10)
HindIII 3647 AAGCTT A^AGCTT
HpaII 2104 CCGG C^CGG
Ksp632I 116 GAAGAG CTCTTC(1/4)
MspA1I 657 CAGCTG C[AC]G^C[GT]G
MspI 2104 CCGG C^CGG
NciI 2104 CCGGG CC^[GC]GG
NcoI 131 CCATGG C^CATGG
NheI 709 GCTAGC G^CTAGC
PacI 3656 TTAATTAA TTAAT^TAA
PflMI 3872 CCAAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3986 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 3760 ATTAAT AT^TAAT
PstI 1493 CTGCAG CTGCA^G
PvuII 657 CAGCTG CAG^CTG
SacI 3052 GAGCTC GAGCT^C
ScaI 3510 AGTACT AGT^ACT
SnaBI 1972 TACGTA TAC^GTA
SphI 3500 GCATGC GCATG^C
SspBI 1478 TGTACA T^GTACA
Van91I 3872 CCAAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1247 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanII 1 GGGCTC G[GA]GC[CT]^C
3052 GAGCTC
BciVI 499 GGATAC GTATCC(6/5)
2235 GTATCC
BsaBI 1326 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2703 GATTAGCATC
BspLU11I 1249 ACATGT A^CATGT
3917 ACATGT
BspMI 1124 ACCTGC ACCTGC(4/8)
545 GCAGGT
BsrBI 20 CCGCTC CCGCTC(-3/-3)
25 CCGCTC
BstXI 2497 CCAGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3847 CCATAAAAATGG
BstYI 386 AGATCT [GA]^GATC[CT]
805 GGATCC
EcoT22I 1436 ATGCAT ATGCA^T
1924 ATGCAT
HaeIII 13 GGCC GG^CC
3844 GGCC
HpaI 239 GTTAAC GTT^AAC
3380 GTTAAC
MamI 1326 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2703 GATTAGCATC
MflI 386 AGATCT [GA]^GATC[CT]
805 GGATCC
MslI 3620 CATACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3848 CATAAAAATG
NsiI 1436 ATGCAT ATGCA^T
1924 ATGCAT
SexAI 427 ACCTGGT A^CC[AT]GGT
1285 ACCTGGT
XbaI 383 TCTAGA T^CTAGA
4084 TCTAGA
XhoII 386 AGATCT [GA]^GATC[CT]
805 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BbvI
BclI BfaI BmyI BpmI BsaJI
BsiHKAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI EcoRII EcoT14I
FbaI Fnu4HI FokI HincII HindII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I