##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       Acc65I      AccI        AcyI        AgeI        
Aor51HI     ApaI        AscI        Asp718I     AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BcgI        BciVI       BclI        BglII       
BlnI        BlpI        Bpu1102I    BsaHI       BsiEI       
BsiWI       BsmBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     CelII       Cfr10I      CpoI        EagI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HpaI        KasI        KpnI        KspI        
MfeI        MluI        MluNI       MscI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PshAI       PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tth111I     Van91I      XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccII         612  CGCG                 CG^CG
AccIII        538  TCCGGA               T^CCGGA
AflII        1822  CTTAAG               C^TTAAG
Alw44I        836  GTGCAC               G^TGCAC
AocI          258  CCTGAGG              CC^T[ACGT]AGG
ApaLI         836  GTGCAC               G^TGCAC
AspHI         836  GTGCAC               G[AT]GC[AT]^C
BanII        1704  GGGCTC               G[GA]GC[CT]^C
BbrPI         966  CACGTG               CAC^GTG
BbsI         1648  GTCTTC               GAAGAC(2/6)
BbvCI           3  CCTCAGC              CCTCAGC(-5/-2)
BfrI         1822  CTTAAG               C^TTAAG
BglI          948  GCCAACCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1648  GTCTTC               GAAGAC(2/6)
BsaBI        1888  GATGGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          399  GAGACC               GGTCTC(1/5)
BsaWI         538  TCCGGA               [AT]^CCGG[AT]
BseAI         538  TCCGGA               T^CCGGA
BsiHKAI       836  GTGCAC               G[AT]GC[AT]^C
Bsp1407I      889  TGTACA               T^GTACA
BspDI         729  ATCGAT               AT^CGAT
BspEI         538  TCCGGA               T^CCGGA
BspHI         472  TCATGA               T^CATGA
BspLU11I      886  ACATGT               A^CATGT
BspMI         251  GCAGGT               ACCTGC(4/8)
BsrBI          60  CCGCTC               CCGCTC(-3/-3)
BsrGI         889  TGTACA               T^GTACA
BstUI         612  CGCG                 CG^CG
Bsu36I        258  CCTGAGG              CC^T[ACGT]AGG
CfoI          527  GCGC                 GCG^C
ClaI          729  ATCGAT               AT^CGAT
DraIII       1236  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          341  GACCTCGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          222  CGGCCA               [CT]^GGCC[GA]
Eco81I        258  CCTGAGG              CC^T[ACGT]AGG
EcoNI         788  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1351  GAATTC               G^AATTC
EcoT22I      1798  ATGCAT               ATGCA^T
HaeII         526  GGCGCT               [GA]GCGC^[CT]
HhaI          527  GCGC                 GCG^C
HinP1I        527  GCGC                 G^CGC
HindIII       935  AAGCTT               A^AGCTT
MamI         1888  GATGGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          538  TCCGGA               T^CCGGA
MvnI          612  CGCG                 CG^CG
NsiI         1798  ATGCAT               ATGCA^T
PacI         2680  TTAATTAA             TTAAT^TAA
PmaCI         966  CACGTG               CAC^GTG
PmlI          966  CACGTG               CAC^GTG
RcaI          472  TCATGA               T^CATGA
ScaI          499  AGTACT               AGT^ACT
SmaI          390  CCCGGG               CCC^GGG
SmlI         1822  CTTAAG               C^T[CT][GA]AG
SspBI         889  TGTACA               T^GTACA
StuI          262  AGGCCT               AGG^CCT
XbaI         1724  TCTAGA               T^CTAGA
XmaCI         390  CCCGGG               C^CCGGG
XmaI          390  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AclI         1758  AACGTT               AA^CGTT
             2675  AACGTT              
AflIII        886  ACATGT               A^C[GA][CT]GT
              967  ACGTGT              
AhdI         1284  GACTGAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2265  GACTGAAAGTC         
AlwNI         193  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1014  CAGACTCTG           
Asp700I       905  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1755  GAAAACGTTC          
AspEI        1284  GACTGAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2265  GACTGAAAGTC         
AvaII         254  GGTCC                G^G[AT]CC
             1390  GGTCC               
BmyI          836  GTGCAC               G[AGT]GC[ACT]^C
             1704  GGGCTC              
BsaAI         550  TACGTG               [CT]AC^GT[GA]
              966  CACGTG              
BsmI         1796  GAATGC               GAATGC(1/-1)
              145  GCATTC              
Bsp1286I      836  GTGCAC               G[AGT]GC[ACT]^C
             1704  GGGCTC              
BstYI         215  AGATCC               [GA]^GATC[CT]
              565  AGATCC              
Eam1105I     1284  GACTGAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2265  GACTGAAAGTC         
FauI           59  CCCGC                CCCGC(4/6)
              961  CCCGC               
MflI          215  AGATCC               [GA]^GATC[CT]
              565  AGATCC              
PpuMI         253  AGGTCCC              [GA]G^G[AT]CC[CT]
             1389  AGGTCCT             
Psp1406I     1758  AACGTT               AA^CGTT
             2675  AACGTT              
XhoII         215  AGATCC               [GA]^GATC[CT]
              565  AGATCC              
XmnI          905  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1755  GAAAACGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AseI        AsnI        AvaI        BbvI        
BcnI        BfaI        BpmI        BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
BsoBI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PshBI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I