##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbrPI BbvCI BglII BlnI
BsaAI BsaBI BsiWI BsmBI BsoBI
BspDI BssHII BssSI Bst1107I BstBI
BstYI BstZ17I ClaI CpoI DraIII
DrdI Eam1105I Eco47III EcoRV EcoT14I
FspI HpaI KpnI KspI MamI
MfeI MflI MluI MunI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StyI SwaI
Tth111I Van91I XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 2987 GTCTAC GT^[AC][GT]AC
AccIII 461 TCCGGA T^CCGGA
AflII 3617 CTTAAG C^TTAAG
AocI 995 CCTGAGG CC^T[ACGT]AGG
BalI 423 TGGCCA TGG^CCA
BbeI 375 GGCGCC GGCGC^C
BcgI 1312 CGAATCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2711 GGATAC GTATCC(6/5)
BfrI 3617 CTTAAG C^TTAAG
BglI 367 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 571 GCTGAGC GC^T[ACGT]AGC
BpmI 41 CTGGAG CTGGAG(16/14)
Bpu1102I 571 GCTGAGC GC^T[ACGT]AGC
BsaI 890 GAGACC GGTCTC(1/5)
BsaWI 461 TCCGGA [AT]^CCGG[AT]
BseAI 461 TCCGGA T^CCGGA
BsmI 988 GAATGC GAATGC(1/-1)
Bsp1407I 2320 TGTACA T^GTACA
BspEI 461 TCCGGA T^CCGGA
BsrGI 2320 TGTACA T^GTACA
BstAPI 282 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2313 GGTCACC G^GT[ACGT]ACC
BstPI 2313 GGTCACC G^GT[ACGT]ACC
BstXI 201 CCACGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 995 CCTGAGG CC^T[ACGT]AGG
CelII 571 GCTGAGC GC^T[ACGT]AGC
EagI 453 CGGCCG C^GGCCG
EclXI 453 CGGCCG C^GGCCG
Eco52I 453 CGGCCG C^GGCCG
Eco81I 995 CCTGAGG CC^T[ACGT]AGG
EcoNI 859 CCTAAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2313 GGTCACC G^GT[ACGT]ACC
EcoT22I 1704 ATGCAT ATGCA^T
FseI 450 GGCCGGCC GGCCGG^CC
HgaI 382 GACGC GACGC(5/10)
HincII 1973 GTTGAC GT[CT]^[GA]AC
HindII 1973 GTTGAC GT[CT]^[GA]AC
KasI 375 GGCGCC G^GCGCC
MluNI 423 TGGCCA TGG^CCA
MroI 461 TCCGGA T^CCGGA
MscI 423 TGGCCA TGG^CCA
NarI 375 GGCGCC GG^CGCC
NsiI 1704 ATGCAT ATGCA^T
PpuMI 2752 AGGACCT [GA]G^G[AT]CC[CT]
SacI 2481 GAGCTC GAGCT^C
SapI 31 GCTCTTC GCTCTTC(1/4)
SfoI 375 GGCGCC GGC^GCC
SpeI 4204 ACTAGT A^CTAGT
SspBI 2320 TGTACA T^GTACA
StuI 144 AGGCCT AGG^CCT
XbaI 4682 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 375 GGCGCC G[GA]^CG[CT]C
382 GACGCC
AflIII 821 ACATGT A^C[GA][CT]GT
1148 ACATGT
AlwI 4063 GATCC GGATC(4/5)
3343 GGATC
Asp700I 4291 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4496 GAAATAATTC
AspHI 2481 GAGCTC G[AT]GC[AT]^C
4200 GAGCAC
AvaII 336 GGACC G^G[AT]CC
2753 GGACC
BanI 375 GGCGCC G^G[CT][GA]CC
1457 GGTGCC
BclI 2998 TGATCA T^GATCA
3922 TGATCA
BsaHI 375 GGCGCC G[GA]^CG[CT]C
382 GACGCC
BsiEI 274 CGGTCG CG[GA][CT]^CG
453 CGGCCG
BsiHKAI 2481 GAGCTC G[AT]GC[AT]^C
4200 GAGCAC
BspLU11I 821 ACATGT A^CATGT
1148 ACATGT
BspMI 2568 ACCTGC ACCTGC(4/8)
3514 ACCTGC
DraII 404 GGGGCCT [GA]G^G[ACGT]CC[CT]
2752 AGGACCT
DsaI 174 CCGTGG C^C[GA][CT]GG
251 CCGTGG
EaeI 423 TGGCCA [CT]^GGCC[GA]
453 CGGCCG
EcoO109I 404 GGGGCCT [GA]G^G[ACGT]CC[CT]
2752 AGGACCT
EcoRI 1617 GAATTC G^AATTC
4156 GAATTC
FbaI 2998 TGATCA T^GATCA
3922 TGATCA
HaeII 233 GGCGCT [GA]GCGC^[CT]
375 GGCGCC
Hin1I 375 GGCGCC G[GA]^CG[CT]C
382 GACGCC
HindIII 3165 AAGCTT A^AGCTT
4803 AAGCTT
NaeI 324 GCCGGC GCC^GGC
451 GCCGGC
NgoMIV 324 GCCGGC G^CCGGC
451 GCCGGC
PvuII 362 CAGCTG CAG^CTG
656 CAGCTG
TaqI 505 TCGA T^CGA
1168 TCGA
TthHB8I 505 TCGA T^CGA
1168 TCGA
XmnI 4291 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4496 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AclI AcsI AfaI
AluI AlwNI ApoI AseI AsnI
BanII BbsI BbvI BcnI BfaI
BmyI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PshBI
Psp1406I PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI