##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       Acc65I      AccII       AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BclI        BfrI        BlnI        
BlpI        Bpu1102I    BsaBI       BsiEI       BsiWI       
BspDI       BsrBI       BssHII      BstBI       BstUI       
CelII       ClaI        CpoI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MunI        MvnI        NaeI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       PvuI        RsrII       SacI        
SacII       SalI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        StuI        SwaI        TaqI        
TthHB8I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII       1451  TCCGGA               T^CCGGA
AclI         1668  AACGTT               AA^CGTT
AcyI         1711  GGCGCC               G[GA]^CG[CT]C
AflIII       1411  ACATGT               A^C[GA][CT]GT
AspI         2492  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2839  TGGCCA               TGG^CCA
BbeI         1711  GGCGCC               GGCGC^C
BbrPI         615  CACGTG               CAC^GTG
BbvCI        1740  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1454  GGATAC               GTATCC(6/5)
BglII        2754  AGATCT               A^GATCT
BsaAI         615  CACGTG               [CT]AC^GT[GA]
BsaHI        1711  GGCGCC               G[GA]^CG[CT]C
BsaWI        1451  TCCGGA               [AT]^CCGG[AT]
BseAI        1451  TCCGGA               T^CCGGA
BsmBI         800  CGTCTC               CGTCTC(1/5)
BsmI         1547  GAATGC               GAATGC(1/-1)
Bsp1407I     2234  TGTACA               T^GTACA
BspEI        1451  TCCGGA               T^CCGGA
BspLU11I     1411  ACATGT               A^CATGT
BsrGI        2234  TGTACA               T^GTACA
DrdI          792  GACTATTCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I        1711  GGCGCC               G[GA]^CG[CT]C
HincII        717  GTCAAC               GT[CT]^[GA]AC
HindII        717  GTCAAC               GT[CT]^[GA]AC
KasI         1711  GGCGCC               G^GCGCC
MluNI        2839  TGGCCA               TGG^CCA
MroI         1451  TCCGGA               T^CCGGA
MscI         2839  TGGCCA               TGG^CCA
NarI         1711  GGCGCC               GG^CGCC
NheI         2718  GCTAGC               G^CTAGC
PflFI        2492  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI         2294  GAGTC                GAGTC(4/5)
PmaCI         615  CACGTG               CAC^GTG
PmlI          615  CACGTG               CAC^GTG
Psp1406I     1668  AACGTT               AA^CGTT
SapI         2477  GCTCTTC              GCTCTTC(1/4)
SbfI         2796  CCTGCAGG             CCTGCA^GG
ScaI          240  AGTACT               AGT^ACT
SexAI        2846  ACCAGGT              A^CC[AT]GGT
SfoI         1711  GGCGCC               GGC^GCC
SmaI         2041  CCCGGG               CCC^GGG
SmlI          735  CTCAAG               C^T[CT][GA]AG
SphI          983  GCATGC               GCATG^C
Sse8387I     2796  CCTGCAGG             CCTGCA^GG
SspBI        2234  TGTACA               T^GTACA
SspI          315  AATATT               AAT^ATT
TfiI         2346  GAATC                G^A[AT]TC
Tth111I      2492  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        2041  CCCGGG               C^CCGGG
XmaI         2041  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcsI         2954  AAATTC               [GA]^AATT[CT]
             3101  AAATTT              
AlwNI        2324  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2791  CAGAGCCTG           
AocI          762  CCTGAGG              CC^T[ACGT]AGG
              834  CCTGAGG             
ApoI         2954  AAATTC               [GA]^AATT[CT]
             3101  AAATTT              
BamHI          21  GGATCC               G^GATCC
             1208  GGATCC              
BanI         1385  GGTGCC               G^G[CT][GA]CC
             1711  GGCGCC              
BbsI           78  GAAGAC               GAAGAC(2/6)
              556  GTCTTC              
BglI         1641  GCCGTGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1721  GCCCCTGCGGC         
BpuAI          78  GAAGAC               GAAGAC(2/6)
              556  GTCTTC              
BsgI         2197  CTGCAC               GTGCAG(16/14)
             2429  CTGCAC              
BsrFI        1220  GCCGGT               [GA]^CCGG[CT]
             1833  GCCGGT              
BssSI        1821  CACGAG               CACGAG(-5/-1)
             1606  CTCGTG              
Bst1107I      875  GTATAC               GTA^TAC
             2161  GTATAC              
BstAPI       1967  GCAGACATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2790  GCAGAGCCTGC         
BstEII       2803  GGTGACC              G^GT[ACGT]ACC
             2850  GGTGACC             
BstPI        2803  GGTGACC              G^GT[ACGT]ACC
             2850  GGTGACC             
BstZ17I       875  GTATAC               GTA^TAC
             2161  GTATAC              
Bsu36I        762  CCTGAGG              CC^T[ACGT]AGG
              834  CCTGAGG             
Cfr10I       1220  GCCGGT               [GA]^CCGG[CT]
             1833  GCCGGT              
EaeI         1515  CGGCCA               [CT]^GGCC[GA]
             2839  TGGCCA              
EarI          933  CTCTTC               CTCTTC(1/4)
             2478  CTCTTC              
Eco81I        762  CCTGAGG              CC^T[ACGT]AGG
              834  CCTGAGG             
EcoNI        1104  CCTATGGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2581  CCTCTGGAAGG         
EcoO65I      2803  GGTGACC              G^GT[ACGT]ACC
             2850  GGTGACC             
HgaI         2285  GACGC                GACGC(5/10)
              382  GCGTC               
HinfI        2294  GAGTC                G^A[ACGT]TC
             2346  GAATC               
Ksp632I       933  CTCTTC               CTCTTC(1/4)
             2478  CTCTTC              
NcoI         2695  CCATGG               C^CATGG
             3079  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccI        AciI        AfaI        AluI        AlwI        
ApaI        AspHI       AvaI        AvaII       BanII       
BbvI        BcgI        BcnI        BfaI        BmyI        
BpmI        BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspHI       BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PpuMI       PstI        PvuII       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Van91I      XcmI        XhoII