##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AccII AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AspEI AspI AviII BamHI
BbeI BbrPI BbvCI BclI BfrI
BglI BlpI Bpu1102I BsaAI BsiEI
BsmBI BspDI BsrBI BsrFI BssHII
Bst1107I BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HpaI KasI KspI MluI MspA1I
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 3249 GACGTC GACGT^C
Acc65I 2407 GGTACC G^GTACC
AccIII 1181 TCCGGA T^CCGGA
AlwNI 446 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 2407 GGTACC G^GTACC
AvaI 1745 CTCGGG C^[CT]CG[GA]G
AvrII 3664 CCTAGG C^CTAGG
BalI 1512 TGGCCA TGG^CCA
BanII 3456 GAGCTC G[GA]GC[CT]^C
BciVI 1036 GGATAC GTATCC(6/5)
BcnI 36 CCCGG CC^[GC]GG
BglII 4170 AGATCT A^GATCT
BlnI 3664 CCTAGG C^CTAGG
BsaBI 127 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3430 GAGACC GGTCTC(1/5)
BseAI 1181 TCCGGA T^CCGGA
BseRI 3623 CTCCTC GAGGAG(10/8)
BsiWI 2959 CGTACG C^GTACG
BsoBI 1745 CTCGGG C^[CT]CG[GA]G
BspEI 1181 TCCGGA T^CCGGA
BspHI 4147 TCATGA T^CATGA
BssSI 1738 CACGAG CACGAG(-5/-1)
BstAPI 4366 GCAAAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3403 TTCGAA TT^CGAA
DrdI 324 GACATTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1512 TGGCCA [CT]^GGCC[GA]
EcoRV 1306 GATATC GAT^ATC
HgaI 1233 GCGTC GACGC(5/10)
HincII 2884 GTCAAC GT[CT]^[GA]AC
HindII 2884 GTCAAC GT[CT]^[GA]AC
KpnI 2407 GGTACC GGTAC^C
MamI 127 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1512 TGGCCA TGG^CCA
MroI 1181 TCCGGA T^CCGGA
MscI 1512 TGGCCA TGG^CCA
NciI 36 CCCGG CC^[GC]GG
NcoI 284 CCATGG C^CATGG
NdeI 3913 CATATG CA^TATG
NspV 3403 TTCGAA TT^CGAA
PstI 1331 CTGCAG CTGCA^G
RcaI 4147 TCATGA T^CATGA
SacI 3456 GAGCTC GAGCT^C
SfuI 3403 TTCGAA TT^CGAA
SmlI 3712 CTTGAG C^T[CT][GA]AG
SwaI 4186 ATTTAAAT ATTT^AAAT
XbaI 3329 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2654 GTCTAC GT^[AC][GT]AC
3706 GTCTAC
AciI 954 CCGC CCGC(-3/-1)
1025 CCGC
AcyI 1232 GGCGTC G[GA]^CG[CT]C
3249 GACGTC
AflIII 1056 ACATGT A^C[GA][CT]GT
3215 ACATGT
Asp700I 1069 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1895 GAAACAATTC
AspHI 2947 GAGCAC G[AT]GC[AT]^C
3456 GAGCTC
AvaII 2871 GGTCC G^G[AT]CC
3283 GGTCC
BanI 1524 GGCACC G^G[CT][GA]CC
2407 GGTACC
BmyI 2947 GAGCAC G[AGT]GC[ACT]^C
3456 GAGCTC
BpmI 2458 CTCCAG CTGGAG(16/14)
1505 CTGGAG
BsaHI 1232 GGCGTC G[GA]^CG[CT]C
3249 GACGTC
BsaWI 1181 TCCGGA [AT]^CCGG[AT]
1459 ACCGGA
BsgI 1626 GTGCAG GTGCAG(16/14)
1653 GTGCAG
BsiHKAI 2947 GAGCAC G[AT]GC[AT]^C
3456 GAGCTC
BsmI 3406 GAATGC GAATGC(1/-1)
3175 GCATTC
Bsp1286I 2947 GAGCAC G[AGT]GC[ACT]^C
3456 GAGCTC
BspLU11I 1056 ACATGT A^CATGT
3215 ACATGT
BspMI 804 ACCTGC ACCTGC(4/8)
3492 ACCTGC
BsrDI 2512 CATTGC GCAATG(2/0)
242 GCAATG
BstYI 132 AGATCC [GA]^GATC[CT]
4170 AGATCT
DsaI 284 CCATGG C^C[GA][CT]GG
3802 CCGTGG
EarI 496 CTCTTC CTCTTC(1/4)
1086 CTCTTC
EcoRI 3731 GAATTC G^AATTC
4007 GAATTC
Hin1I 1232 GGCGTC G[GA]^CG[CT]C
3249 GACGTC
HindIII 439 AAGCTT A^AGCTT
2744 AAGCTT
Ksp632I 496 CTCTTC CTCTTC(1/4)
1086 CTCTTC
MflI 132 AGATCC [GA]^GATC[CT]
4170 AGATCT
TaqI 163 TCGA T^CGA
3404 TCGA
TthHB8I 163 TCGA T^CGA
3404 TCGA
XhoII 132 AGATCC [GA]^GATC[CT]
4170 AGATCT
XmnI 1069 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1895 GAAACAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI BbsI BbvI BcgI
BfaI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MfeI
MnlI MseI MslI MspI MunI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I