##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AgeI        Alw44I      Aor51HI     ApaLI       
AscI        Asp700I     Asp718I     AviII       AvrII       
BanI        BbeI        BbrPI       BcgI        BciVI       
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BsrFI       
BssHII      BstEII      BstPI       BstUI       BstXI       
CelII       CfoI        Cfr10I      ClaI        CpoI        
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
FauI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HpaI        KasI        
KpnI        KspI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       PvuI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SgrAI       SnaBI       
Sse8387I    StuI        SwaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI          129  GTATAC               GT^[AC][GT]AC
AclI         3182  AACGTT               AA^CGTT
AhdI          184  GACTCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3098  CCTGAGG              CC^T[ACGT]AGG
ApaI         1254  GGGCCC               GGGCC^C
AseI          814  ATTAAT               AT^TAAT
AsnI          814  ATTAAT               AT^TAAT
AspEI         184  GACTCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          441  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        2075  GGATCC               G^GATCC
BsgI          660  GTGCAG               GTGCAG(16/14)
Bsp1407I     3195  TGTACA               T^GTACA
BspHI         563  TCATGA               T^CATGA
BsrBI        2571  CCGCTC               CCGCTC(-3/-3)
BsrGI        3195  TGTACA               T^GTACA
BssSI         272  CACGAG               CACGAG(-5/-1)
Bst1107I      129  GTATAC               GTA^TAC
BstAPI       2179  GCAGGGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2590  TTCGAA               TT^CGAA
BstZ17I       129  GTATAC               GTA^TAC
Bsu36I       3098  CCTGAGG              CC^T[ACGT]AGG
DraIII       3412  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           86  GACCTCAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      184  GACTCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3098  CCTGAGG              CC^T[ACGT]AGG
EcoNI        3401  CCTGCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         559  GATATC               GAT^ATC
EcoT22I       229  ATGCAT               ATGCA^T
HapII        2583  CCGG                 C^CGG
HincII        203  GTCAAC               GT[CT]^[GA]AC
HindII        203  GTCAAC               GT[CT]^[GA]AC
HindIII       329  AAGCTT               A^AGCTT
HpaII        2583  CCGG                 C^CGG
MspI         2583  CCGG                 C^CGG
NdeI         1907  CATATG               CA^TATG
NsiI          229  ATGCAT               ATGCA^T
NspV         2590  TTCGAA               TT^CGAA
PaeR7I        919  CTCGAG               C^TCGAG
PflFI         441  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        3625  CCATGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         814  ATTAAT               AT^TAAT
Psp1406I     3182  AACGTT               AA^CGTT
PstI            4  CTGCAG               CTGCA^G
RcaI          563  TCATGA               T^CATGA
SfuI         2590  TTCGAA               TT^CGAA
SmaI         2582  CCCGGG               CCC^GGG
SmlI          919  CTCGAG               C^T[CT][GA]AG
SpeI         3843  ACTAGT               A^CTAGT
SphI          459  GCATGC               GCATG^C
SspBI        3195  TGTACA               T^GTACA
SspI         1762  AATATT               AAT^ATT
Tsp45I       3018  GTCAC                ^GT[GC]AC
Tth111I       441  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       3625  CCATGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3604  TCTAGA               T^CTAGA
XhoI          919  CTCGAG               C^TCGAG
XmaCI        2582  CCCGGG               C^CCGGG
XmaI         2582  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AvaI          919  CTCGAG               C^[CT]CG[GA]G
             2582  CCCGGG              
BcnI         2582  CCCGG                CC^[GC]GG
             2583  CCGGG               
BfaI         3605  CTAG                 C^TAG
             3844  CTAG                
BglII         264  AGATCT               A^GATCT
              708  AGATCT              
BsaBI        2076  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3121  GATGAAAATC          
BsaI          439  GAGACC               GGTCTC(1/5)
              510  GAGACC              
BseRI         536  CTCCTC               GAGGAG(10/8)
             1242  CTCCTC              
BsmI         2643  GAATGC               GAATGC(1/-1)
             1188  GCATTC              
BsoBI         919  CTCGAG               C^[CT]CG[GA]G
             2582  CCCGGG              
BspLU11I      143  ACATGT               A^CATGT
             3668  ACATGT              
DsaI         1913  CCATGG               C^C[GA][CT]GG
             2071  CCATGG              
EcoRI        1378  GAATTC               G^AATTC
             3600  GAATTC              
MaeI         3605  CTAG                 C^TAG
             3844  CTAG                
MamI         2076  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3121  GATGAAAATC          
NciI         2582  CCCGG                CC^[GC]GG
             2583  CCGGG               
NcoI         1913  CCATGG               C^CATGG
             2071  CCATGG              
PpuMI         281  GGGACCT              [GA]G^G[AT]CC[CT]
             4044  AGGACCC             
SacI         2156  GAGCTC               GAGCT^C
             3916  GAGCTC              
SexAI        1160  ACCTGGT              A^CC[AT]GGT
             2246  ACCAGGT             
TaqI          920  TCGA                 T^CGA
             2591  TCGA                
TthHB8I       920  TCGA                 T^CGA
             2591  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BalI        BanII       BbsI        BbvCI       BbvI        
BclI        BmyI        BpmI        BpuAI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FbaI        
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       XhoII