##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AflIII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI AvrII BciVI BclI BglI
BglII BlnI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoRV EcoT22I FbaI FseI HpaI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SfiI SfuI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1243 GTAGAC GT^[AC][GT]AC
AcsI 3744 GAATTC [GA]^AATT[CT]
AflII 3602 CTTAAG C^TTAAG
Aor51HI 1760 AGCGCT AGC^GCT
ApoI 3744 GAATTC [GA]^AATT[CT]
AviII 1616 TGCGCA TGC^GCA
BbeI 3381 GGCGCC GGCGC^C
BbrPI 1612 CACGTG CAC^GTG
BcgI 1317 GCATCGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3602 CTTAAG C^TTAAG
BlpI 661 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 661 GCTGAGC GC^T[ACGT]AGC
BsaAI 1612 CACGTG [CT]AC^GT[GA]
BsaBI 1976 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3338 CGTCTC CGTCTC(1/5)
BsmI 490 GAATGC GAATGC(1/-1)
Bsp1407I 1156 TGTACA T^GTACA
BsrBI 15 GAGCGG CCGCTC(-3/-3)
BsrGI 1156 TGTACA T^GTACA
BssSI 3291 CACGAG CACGAG(-5/-1)
CelII 661 GCTGAGC GC^T[ACGT]AGC
DraI 3218 TTTAAA TTT^AAA
DraIII 2267 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2248 GACAAACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1760 AGCGCT AGC^GCT
EcoRI 3744 GAATTC G^AATTC
FspI 1616 TGCGCA TGC^GCA
HindIII 2495 AAGCTT A^AGCTT
KasI 3381 GGCGCC G^GCGCC
KspI 1254 CCGCGG CCGC^GG
MamI 1976 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3381 GGCGCC GG^CGCC
PaeR7I 3733 CTCGAG C^TCGAG
PmaCI 1612 CACGTG CAC^GTG
PmlI 1612 CACGTG CAC^GTG
SacII 1254 CCGCGG CCGC^GG
SexAI 2396 ACCAGGT A^CC[AT]GGT
SfoI 3381 GGCGCC GGC^GCC
SmaI 3434 CCCGGG CCC^GGG
SphI 2754 GCATGC GCATG^C
SspBI 1156 TGTACA T^GTACA
XcmI 2504 CCAGGTCTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3733 CTCGAG C^TCGAG
XmaCI 3434 CCCGGG C^CCGGG
XmaI 3434 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 1565 GGCGTC G[GA]^CG[CT]C
3381 GGCGCC
AspI 3771 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3942 GACCCTGTC
BamHI 2279 GGATCC G^GATCC
2938 GGATCC
BbsI 92 GAAGAC GAAGAC(2/6)
1174 GTCTTC
BfaI 823 CTAG C^TAG
2989 CTAG
BpuAI 92 GAAGAC GAAGAC(2/6)
1174 GTCTTC
BsaHI 1565 GGCGTC G[GA]^CG[CT]C
3381 GGCGCC
BsrDI 1313 CATTGC GCAATG(2/0)
839 GCAATG
BstEII 2400 GGTCACC G^GT[ACGT]ACC
2475 GGTAACC
BstPI 2400 GGTCACC G^GT[ACGT]ACC
2475 GGTAACC
EcoO65I 2400 GGTCACC G^GT[ACGT]ACC
2475 GGTAACC
Hin1I 1565 GGCGTC G[GA]^CG[CT]C
3381 GGCGCC
HincII 222 GTCAAC GT[CT]^[GA]AC
255 GTCAAC
HindII 222 GTCAAC GT[CT]^[GA]AC
255 GTCAAC
MaeI 823 CTAG C^TAG
2989 CTAG
NaeI 1019 GCCGGC GCC^GGC
1384 GCCGGC
NgoMIV 1019 GCCGGC G^CCGGC
1384 GCCGGC
PflFI 3771 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3942 GACCCTGTC
PflMI 1740 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2070 CCAGCCACTGG
PpuMI 2236 AGGACCT [GA]G^G[AT]CC[CT]
2683 AGGTCCT
StuI 2891 AGGCCT AGG^CCT
3674 AGGCCT
TfiI 2819 GATTC G^A[AT]TC
3020 GAATC
Tth111I 3771 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3942 GACCCTGTC
Van91I 1740 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2070 CCAGCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SgrAI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII