##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
AocI Aor51HI ApaLI AscI AvaI
AviII AvrII BbeI BbrPI BbvCI
BfrI BlnI BlpI Bpu1102I BsaBI
BsiWI BsmI BsoBI BspDI BspHI
BsrDI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I CelII ClaI CpoI
Eco47III Eco81I EcoRI EcoRV FseI
FspI HindIII KasI MamI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmlI PshAI Psp1406I
PvuI RcaI RsrII SalI SfoI
SfuI SgrAI SmaI SnaBI SpeI
StuI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 162 GGTACC G^GTACC
AccI 3624 GTCTAC GT^[AC][GT]AC
AccIII 379 TCCGGA T^CCGGA
ApaI 303 GGGCCC GGGCC^C
AseI 2264 ATTAAT AT^TAAT
AsnI 2264 ATTAAT AT^TAAT
Asp718I 162 GGTACC G^GTACC
AspI 1711 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1059 GGATCC G^GATCC
BcgI 3353 CGAGTTGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3392 GTATCC GTATCC(6/5)
BclI 2866 TGATCA T^GATCA
BsaAI 1112 TACGTG [CT]AC^GT[GA]
BseAI 379 TCCGGA T^CCGGA
BsmBI 65 GAGACG CGTCTC(1/5)
BspEI 379 TCCGGA T^CCGGA
BsrFI 275 GCCGGC [GA]^CCGG[CT]
BstAPI 1696 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3889 GGTGACC G^GT[ACGT]ACC
BstPI 3889 GGTGACC G^GT[ACGT]ACC
BstXI 736 CCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 275 GCCGGC [GA]^CCGG[CT]
DraIII 1736 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3715 GACAGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3889 GGTGACC G^GT[ACGT]ACC
EcoT22I 1726 ATGCAT ATGCA^T
FbaI 2866 TGATCA T^GATCA
HpaI 3088 GTTAAC GTT^AAC
KpnI 162 GGTACC GGTAC^C
MroI 379 TCCGGA T^CCGGA
NaeI 275 GCCGGC GCC^GGC
NcoI 736 CCATGG C^CATGG
NgoMIV 275 GCCGGC G^CCGGC
NsiI 1726 ATGCAT ATGCA^T
PflFI 1711 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 958 GTTTAAAC GTTT^AAAC
PpuMI 2838 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2264 ATTAAT AT^TAAT
SexAI 2043 ACCAGGT A^CC[AT]GGT
SfiI 274 GGCCGGCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 2967 CTTGAG C^T[CT][GA]AG
SphI 1724 GCATGC GCATG^C
SspI 3380 AATATT AAT^ATT
Tth111I 1711 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2104 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 3920 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3940 GACAACACGTC
Asp700I 1973 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3551 GAACAGTTTC
AspEI 3920 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3940 GACAACACGTC
BanI 162 GGTACC G^G[CT][GA]CC
2807 GGTGCC
BglII 387 AGATCT A^GATCT
3428 AGATCT
BsaI 884 GAGACC GGTCTC(1/5)
1130 GGTCTC
BsaWI 379 TCCGGA [AT]^CCGG[AT]
555 TCCGGT
BsiEI 183 CGGCCG CG[GA][CT]^CG
282 CGGCCG
Bsp1407I 1537 TGTACA T^GTACA
2230 TGTACA
BspLU11I 1065 ACATGT A^CATGT
1407 ACATGT
BsrBI 1226 CCGCTC CCGCTC(-3/-3)
75 GAGCGG
BsrGI 1537 TGTACA T^GTACA
2230 TGTACA
DraII 627 AGGCCCT [GA]G^G[ACGT]CC[CT]
2838 GGGTCCC
EagI 183 CGGCCG C^GGCCG
282 CGGCCG
Eam1105I 3920 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3940 GACAACACGTC
EclXI 183 CGGCCG C^GGCCG
282 CGGCCG
Eco52I 183 CGGCCG C^GGCCG
282 CGGCCG
EcoNI 172 CCTGATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
265 CCTCCGCCAGG
EcoO109I 627 AGGCCCT [GA]G^G[ACGT]CC[CT]
2838 GGGTCCC
HaeII 99 AGCGCC [GA]GCGC^[CT]
2413 GGCGCT
HincII 559 GTCAAC GT[CT]^[GA]AC
3088 GTTAAC
HindII 559 GTCAAC GT[CT]^[GA]AC
3088 GTTAAC
SbfI 928 CCTGCAGG CCTGCA^GG
3268 CCTGCAGG
ScaI 252 AGTACT AGT^ACT
2014 AGTACT
Sse8387I 928 CCTGCAGG CCTGCA^GG
3268 CCTGCAGG
SspBI 1537 TGTACA T^GTACA
2230 TGTACA
XcmI 727 CCAGCCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1320 CCAAGGAGCTCTTGG
XmnI 1973 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3551 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BalI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII