##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BcnI BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I FauI FseI
FspI HpaI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SbfI
SfiI SfuI SgrAI SmaI SpeI
Sse8387I StuI SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflII 6885 CTTAAG C^TTAAG
AocI 1406 CCTCAGG CC^T[ACGT]AGG
AseI 1922 ATTAAT AT^TAAT
AsnI 1922 ATTAAT AT^TAAT
BanI 2658 GGCGCC G^G[CT][GA]CC
BbeI 2658 GGCGCC GGCGC^C
BcgI 5830 CGAAAGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 6885 CTTAAG C^TTAAG
BglI 7719 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 7935 TACGTA [CT]AC^GT[GA]
BsaI 6819 GAGACC GGTCTC(1/5)
Bsp1407I 7598 TGTACA T^GTACA
BsrFI 4416 GCCGGT [GA]^CCGG[CT]
BsrGI 7598 TGTACA T^GTACA
BstAPI 2678 GCATATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1406 CCTCAGG CC^T[ACGT]AGG
CfoI 2659 GCGC GCG^C
Cfr10I 4416 GCCGGT [GA]^CCGG[CT]
DraIII 4962 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 954 CCATGG C^C[GA][CT]GG
Eco81I 1406 CCTCAGG CC^T[ACGT]AGG
EcoRV 4253 GATATC GAT^ATC
EcoT22I 6507 ATGCAT ATGCA^T
HaeII 2658 GGCGCC [GA]GCGC^[CT]
HapII 4417 CCGG C^CGG
HhaI 2659 GCGC GCG^C
HinP1I 2659 GCGC G^CGC
HpaII 4417 CCGG C^CGG
KasI 2658 GGCGCC G^GCGCC
MspI 4417 CCGG C^CGG
NarI 2658 GGCGCC GG^CGCC
NcoI 954 CCATGG C^CATGG
NsiI 6507 ATGCAT ATGCA^T
PaeR7I 5601 CTCGAG C^TCGAG
PshBI 1922 ATTAAT AT^TAAT
PstI 5343 CTGCAG CTGCA^G
SalI 2865 GTCGAC G^TCGAC
ScaI 7943 AGTACT AGT^ACT
SexAI 1263 ACCAGGT A^CC[AT]GGT
SfoI 2658 GGCGCC GGC^GCC
SnaBI 7935 TACGTA TAC^GTA
SphI 3929 GCATGC GCATG^C
SspBI 7598 TGTACA T^GTACA
XhoI 5601 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 16 GACGTC GACGT^C
2853 GACGTC
AflIII 3459 ACATGT A^C[GA][CT]GT
7451 ACATGT
Alw44I 4750 GTGCAC G^TGCAC
5838 GTGCAC
ApaLI 4750 GTGCAC G^TGCAC
5838 GTGCAC
AvaI 3003 CTCGGG C^[CT]CG[GA]G
5601 CTCGAG
BalI 4396 TGGCCA TGG^CCA
6712 TGGCCA
BbvCI 399 CCTCAGC CCTCAGC(-5/-2)
3047 GCTGAGG
BclI 364 TGATCA T^GATCA
3915 TGATCA
BglII 4609 AGATCT A^GATCT
5677 AGATCT
BlpI 3146 GCTCAGC GC^T[ACGT]AGC
3465 GCTTAGC
Bpu1102I 3146 GCTCAGC GC^T[ACGT]AGC
3465 GCTTAGC
BsgI 5991 CTGCAC GTGCAG(16/14)
3554 GTGCAG
BsoBI 3003 CTCGGG C^[CT]CG[GA]G
5601 CTCGAG
BspLU11I 3459 ACATGT A^CATGT
7451 ACATGT
BspMI 2509 ACCTGC ACCTGC(4/8)
5341 ACCTGC
BsrBI 3347 GAGCGG CCGCTC(-3/-3)
5906 GAGCGG
BstEII 390 GGTTACC G^GT[ACGT]ACC
4277 GGTGACC
BstPI 390 GGTTACC G^GT[ACGT]ACC
4277 GGTGACC
CelII 3146 GCTCAGC GC^T[ACGT]AGC
3465 GCTTAGC
DrdI 5017 GACTCCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6259 GACTAATGAGTC
EaeI 4396 TGGCCA [CT]^GGCC[GA]
6712 TGGCCA
EcoO65I 390 GGTTACC G^GT[ACGT]ACC
4277 GGTGACC
EcoRI 771 GAATTC G^AATTC
2891 GAATTC
FbaI 364 TGATCA T^GATCA
3915 TGATCA
MluNI 4396 TGGCCA TGG^CCA
6712 TGGCCA
MscI 4396 TGGCCA TGG^CCA
6712 TGGCCA
PpuMI 5439 AGGACCT [GA]G^G[AT]CC[CT]
6456 AGGACCT
SacI 3144 GAGCTC GAGCT^C
7095 GAGCTC
SspI 3599 AATATT AAT^ATT
5195 AATATT
XcmI 592 CCACCCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6170 CCAGATGGAGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanII BbsI BbvI
BciVI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BspHI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI Hin1I
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII XmnI