##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccI AclI AgeI AscI
AseI AsnI Asp700I Asp718I AspI
BciVI BclI BglII BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EcoRV FbaI FseI HpaI
KpnI KspI MfeI MluI MunI
NdeI NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 2357 TCCGGA T^CCGGA
AcsI 4477 GAATTC [GA]^AATT[CT]
AflII 1205 CTTAAG C^TTAAG
AflIII 669 ACGTGT A^C[GA][CT]GT
Alw44I 2317 GTGCAC G^TGCAC
Aor51HI 301 AGCGCT AGC^GCT
ApaLI 2317 GTGCAC G^TGCAC
ApoI 4477 GAATTC [GA]^AATT[CT]
AviII 393 TGCGCA TGC^GCA
BbrPI 3949 CACGTG CAC^GTG
BbvCI 2850 GCTGAGG CCTCAGC(-5/-2)
BfrI 1205 CTTAAG C^TTAAG
BsaBI 1829 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2357 TCCGGA T^CCGGA
BsiWI 283 CGTACG C^GTACG
BspEI 2357 TCCGGA T^CCGGA
BsrBI 329 GAGCGG CCGCTC(-3/-3)
BstAPI 976 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eco47III 301 AGCGCT AGC^GCT
EcoNI 2790 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4477 GAATTC G^AATTC
EcoT22I 2419 ATGCAT ATGCA^T
FspI 393 TGCGCA TGC^GCA
MamI 1829 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2357 TCCGGA T^CCGGA
NaeI 2590 GCCGGC GCC^GGC
NgoMIV 2590 GCCGGC G^CCGGC
NsiI 2419 ATGCAT ATGCA^T
NspI 1882 GCATGT [GA]CATG^[CT]
PaeR7I 4472 CTCGAG C^TCGAG
PmaCI 3949 CACGTG CAC^GTG
PmlI 3949 CACGTG CAC^GTG
SacI 199 GAGCTC GAGCT^C
XhoI 4472 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 1241 GACGTC GACGT^C
3565 GACGTC
ApaI 1456 GGGCCC GGGCC^C
2583 GGGCCC
AvrII 14 CCTAGG C^CTAGG
3886 CCTAGG
BcgI 375 GCAACGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3845 GCATCGCCATCG
BlnI 14 CCTAGG C^CTAGG
3886 CCTAGG
BsaAI 668 TACGTG [CT]AC^GT[GA]
3949 CACGTG
BssHII 135 GCGCGC G^CGCGC
368 GCGCGC
BssSI 614 CACGAG CACGAG(-5/-1)
2630 CACGAG
DraIII 653 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2842 CACCTGGTG
HincII 740 GTCAAC GT[CT]^[GA]AC
3379 GTCAAC
HindII 740 GTCAAC GT[CT]^[GA]AC
3379 GTCAAC
HindIII 2435 AAGCTT A^AGCTT
3096 AAGCTT
PflMI 1609 CCAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4121 CCAGCCCCTGG
PleI 3639 GACTC GAGTC(4/5)
2866 GAGTC
SapI 481 GCTCTTC GCTCTTC(1/4)
2442 GCTCTTC
SmaI 759 CCCGGG CCC^GGG
2896 CCCGGG
Van91I 1609 CCAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4121 CCAGCCCCTGG
XmaCI 759 CCCGGG C^CCGGG
2896 CCCGGG
XmaI 759 CCCGGG C^CCGGG
2896 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AocI AspEI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XcmI XhoII