##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BglI BlnI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI FauI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NgoMIV NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SnaBI SphI Sse8387I
SspBI SwaI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1773 GTCTAC GT^[AC][GT]AC
AciI 1276 CCGC CCGC(-3/-1)
AclI 455 AACGTT AA^CGTT
AseI 2749 ATTAAT AT^TAAT
AsnI 2749 ATTAAT AT^TAAT
Asp700I 4546 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1572 CCCGGG C^[CT]CG[GA]G
BalI 1512 TGGCCA TGG^CCA
BanI 3462 GGCACC G^G[CT][GA]CC
BanII 1334 GAGCTC G[GA]GC[CT]^C
BbvCI 1603 CCTCAGC CCTCAGC(-5/-2)
BclI 333 TGATCA T^GATCA
BsaI 924 GGTCTC GGTCTC(1/5)
BsgI 541 GTGCAG GTGCAG(16/14)
BsoBI 1572 CCCGGG C^[CT]CG[GA]G
DsaI 1487 CCATGG C^C[GA][CT]GG
EaeI 1512 TGGCCA [CT]^GGCC[GA]
EarI 4573 GAAGAG CTCTTC(1/4)
EcoT22I 2810 ATGCAT ATGCA^T
FbaI 333 TGATCA T^GATCA
HaeII 1260 AGCGCC [GA]GCGC^[CT]
Ksp632I 4573 GAAGAG CTCTTC(1/4)
MluNI 1512 TGGCCA TGG^CCA
MscI 1512 TGGCCA TGG^CCA
NcoI 1487 CCATGG C^CATGG
NheI 219 GCTAGC G^CTAGC
NsiI 2810 ATGCAT ATGCA^T
PflMI 2489 CCATAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1575 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 2749 ATTAAT AT^TAAT
Psp1406I 455 AACGTT AA^CGTT
SacI 1334 GAGCTC GAGCT^C
SapI 4573 GAAGAGC GCTCTTC(1/4)
SexAI 2494 ACCTGGT A^CC[AT]GGT
SmaI 1572 CCCGGG CCC^GGG
SpeI 3959 ACTAGT A^CTAGT
StuI 1303 AGGCCT AGG^CCT
Van91I 2489 CCATAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 18 TCTAGA T^CTAGA
XmaCI 1572 CCCGGG C^CCGGG
XmaI 1572 CCCGGG C^CCGGG
XmnI 4546 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflII 975 CTTAAG C^TTAAG
2113 CTTAAG
AflIII 1815 ACATGT A^C[GA][CT]GT
4075 ACATGT
AlwNI 611 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2212 CAGATACTG
AspHI 1187 GTGCTC G[AT]GC[AT]^C
1334 GAGCTC
BbsI 1128 GAAGAC GAAGAC(2/6)
2804 GAAGAC
BciVI 1997 GGATAC GTATCC(6/5)
4116 GTATCC
BfrI 975 CTTAAG C^TTAAG
2113 CTTAAG
BpuAI 1128 GAAGAC GAAGAC(2/6)
2804 GAAGAC
BsaBI 16 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1665 GATTCAGATC
BsiHKAI 1187 GTGCTC G[AT]GC[AT]^C
1334 GAGCTC
BsmI 624 GAATGC GAATGC(1/-1)
1753 GAATGC
BspLU11I 1815 ACATGT A^CATGT
4075 ACATGT
BspMI 1206 ACCTGC ACCTGC(4/8)
1910 ACCTGC
BsrDI 1170 CATTGC GCAATG(2/0)
4708 CATTGC
BstBI 942 TTCGAA TT^CGAA
3049 TTCGAA
DraII 1575 GGGACCC [GA]G^G[ACGT]CC[CT]
3584 AGGCCCC
EcoO109I 1575 GGGACCC [GA]G^G[ACGT]CC[CT]
3584 AGGCCCC
EcoRV 1457 GATATC GAT^ATC
2767 GATATC
EcoT14I 1487 CCATGG C^C[AT][AT]GG
4041 CCTTGG
HapII 58 CCGG C^CGG
1573 CCGG
HpaI 166 GTTAAC GTT^AAC
2595 GTTAAC
HpaII 58 CCGG C^CGG
1573 CCGG
MaeII 456 ACGT A^CGT
4449 ACGT
MamI 16 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1665 GATTCAGATC
MfeI 176 CAATTG C^AATTG
245 CAATTG
MspA1I 1660 CAGCTG C[AC]G^C[GT]G
3593 CAGCTG
MspI 58 CCGG C^CGG
1573 CCGG
MunI 176 CAATTG C^AATTG
245 CAATTG
NspV 942 TTCGAA TT^CGAA
3049 TTCGAA
PvuII 1660 CAGCTG CAG^CTG
3593 CAGCTG
SfcI 1775 CTACAG C^T[GA][CT]AG
4418 CTACAG
SfuI 942 TTCGAA TT^CGAA
3049 TTCGAA
StyI 1487 CCATGG C^C[AT][AT]GG
4041 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HincII HindII HinfI HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII