##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AvrII       BbrPI       
BbsI        BbvCI       BciVI       BclI        BfrI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsiWI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrDI       BsrGI       BssSI       Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HpaI        KspI        MamI        MfeI        
MluI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I       2861  GGTACC               G^GTACC
Asp718I      2861  GGTACC               G^GTACC
AspI         3157  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         901  TGCGCA               TGC^GCA
BalI         3291  TGGCCA               TGG^CCA
BbeI         2834  GGCGCC               GGCGC^C
BcgI         2572  GCACCGGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI        2212  CGGCCG               CG[GA][CT]^CG
BsmBI         749  GAGACG               CGTCTC(1/5)
BsmI          104  GAATGC               GAATGC(1/-1)
BsrBI         928  GAGCGG               CCGCTC(-3/-3)
BssHII       2191  GCGCGC               G^CGCGC
BstEII       1984  GGTGACC              G^GT[ACGT]ACC
BstPI        1984  GGTGACC              G^GT[ACGT]ACC
CpoI         1760  CGGTCCG              CG^G[AT]CCG
DraI         3556  TTTAAA               TTT^AAA
EagI         2212  CGGCCG               C^GGCCG
EclXI        2212  CGGCCG               C^GGCCG
Eco52I       2212  CGGCCG               C^GGCCG
EcoNI        2050  CCTTCCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1984  GGTGACC              G^GT[ACGT]ACC
FspI          901  TGCGCA               TGC^GCA
KasI         2834  GGCGCC               G^GCGCC
KpnI         2861  GGTACC               GGTAC^C
MaeII        2172  ACGT                 A^CGT
MluNI        3291  TGGCCA               TGG^CCA
MscI         3291  TGGCCA               TGG^CCA
MslI         3298  CACGGGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         2834  GGCGCC               GG^CGCC
NcoI         1300  CCATGG               C^CATGG
PflFI        3157  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RsrII        1760  CGGTCCG              CG^G[AT]CCG
SfoI         2834  GGCGCC               GGC^GCC
SgrAI        1901  CACCGGCG             C[GA]^CCGG[CT]G
SphI          714  GCATGC               GCATG^C
Tth111I      3157  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1172  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccII        2192  CGCG                 CG^CG
             2194  CGCG                
AccIII       3231  TCCGGA               T^CCGGA
             3257  TCCGGA              
BglI         2028  GCCAGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2363  GCCCGGACGGC         
BseAI        3231  TCCGGA               T^CCGGA
             3257  TCCGGA              
BsgI         1950  GTGCAG               GTGCAG(16/14)
             2652  GTGCAG              
BspEI        3231  TCCGGA               T^CCGGA
             3257  TCCGGA              
BstUI        2192  CGCG                 CG^CG
             2194  CGCG                
MroI         3231  TCCGGA               T^CCGGA
             3257  TCCGGA              
MvnI         2192  CGCG                 CG^CG
             2194  CGCG                
NaeI         2214  GCCGGC               GCC^GGC
             2776  GCCGGC              
NgoMIV       2214  GCCGGC               G^CCGGC
             2776  GCCGGC              
NspI          714  GCATGC               [GA]CATG^[CT]
             1809  ACATGC              
PpuMI         979  GGGACCC              [GA]G^G[AT]CC[CT]
             1662  AGGACCT             
TaqI          852  TCGA                 T^CGA
             3147  TCGA                
TthHB8I       852  TCGA                 T^CGA
             3147  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BamHI       BanI        BanII       BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Van91I      XcmI        XhoII       XmaCI       XmaI