################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AvrII BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaBI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI DraIII DrdI Eam1105I Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacII SalI SbfI ScaI SexAI SfiI SfuI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 2861 GGTACC G^GTACC Asp718I 2861 GGTACC G^GTACC AspI 3157 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 901 TGCGCA TGC^GCA BalI 3291 TGGCCA TGG^CCA BbeI 2834 GGCGCC GGCGC^C BcgI 2572 GCACCGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsiEI 2212 CGGCCG CG[GA][CT]^CG BsmBI 749 GAGACG CGTCTC(1/5) BsmI 104 GAATGC GAATGC(1/-1) BsrBI 928 GAGCGG CCGCTC(-3/-3) BssHII 2191 GCGCGC G^CGCGC BstEII 1984 GGTGACC G^GT[ACGT]ACC BstPI 1984 GGTGACC G^GT[ACGT]ACC CpoI 1760 CGGTCCG CG^G[AT]CCG DraI 3556 TTTAAA TTT^AAA EagI 2212 CGGCCG C^GGCCG EclXI 2212 CGGCCG C^GGCCG Eco52I 2212 CGGCCG C^GGCCG EcoNI 2050 CCTTCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1984 GGTGACC G^GT[ACGT]ACC FspI 901 TGCGCA TGC^GCA KasI 2834 GGCGCC G^GCGCC KpnI 2861 GGTACC GGTAC^C MaeII 2172 ACGT A^CGT MluNI 3291 TGGCCA TGG^CCA MscI 3291 TGGCCA TGG^CCA MslI 3298 CACGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 2834 GGCGCC GG^CGCC NcoI 1300 CCATGG C^CATGG PflFI 3157 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC RsrII 1760 CGGTCCG CG^G[AT]CCG SfoI 2834 GGCGCC GGC^GCC SgrAI 1901 CACCGGCG C[GA]^CCGG[CT]G SphI 714 GCATGC GCATG^C Tth111I 3157 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1172 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccII 2192 CGCG CG^CG 2194 CGCG AccIII 3231 TCCGGA T^CCGGA 3257 TCCGGA BglI 2028 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2363 GCCCGGACGGC BseAI 3231 TCCGGA T^CCGGA 3257 TCCGGA BsgI 1950 GTGCAG GTGCAG(16/14) 2652 GTGCAG BspEI 3231 TCCGGA T^CCGGA 3257 TCCGGA BstUI 2192 CGCG CG^CG 2194 CGCG MroI 3231 TCCGGA T^CCGGA 3257 TCCGGA MvnI 2192 CGCG CG^CG 2194 CGCG NaeI 2214 GCCGGC GCC^GGC 2776 GCCGGC NgoMIV 2214 GCCGGC G^CCGGC 2776 GCCGGC NspI 714 GCATGC [GA]CATG^[CT] 1809 ACATGC PpuMI 979 GGGACCC [GA]G^G[AT]CC[CT] 1662 AGGACCT TaqI 852 TCGA T^CGA 3147 TCGA TthHB8I 852 TCGA T^CGA 3147 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BamHI BanI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII XmaCI XmaI