##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AocI ApaI ApoI AscI Asp700I
Asp718I AviII AvrII BamHI BbrPI
BciVI BfrI BglII BlnI BsiWI
BspDI BsrDI Bst1107I BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eco81I EcoRI FseI FspI KpnI
MfeI MluI MunI NaeI NgoMIV
NheI NotI NruI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SexAI SgrAI SmaI SnaBI
SpeI SspI StuI SwaI XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccIII 3197 TCCGGA T^CCGGA
AhdI 213 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 687 GTGCAC G^TGCAC
ApaLI 687 GTGCAC G^TGCAC
AseI 3653 ATTAAT AT^TAAT
AsnI 3653 ATTAAT AT^TAAT
AspEI 213 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 323 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 17 GGCGCC GGCGC^C
BbvCI 2640 GCTGAGG CCTCAGC(-5/-2)
BcgI 1936 GCAGCGAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 877 TGATCA T^GATCA
BglI 2277 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2049 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2049 GCTGAGC GC^T[ACGT]AGC
BsaAI 2963 CACGTA [CT]AC^GT[GA]
BsaBI 2792 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3197 TCCGGA [AT]^CCGG[AT]
BseAI 3197 TCCGGA T^CCGGA
BsmI 2519 GAATGC GAATGC(1/-1)
BspEI 3197 TCCGGA T^CCGGA
BspHI 2179 TCATGA T^CATGA
BsrBI 2787 GAGCGG CCGCTC(-3/-3)
BssHII 26 GCGCGC G^CGCGC
BssSI 3580 CACGAG CACGAG(-5/-1)
BstAPI 3124 GCAAACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2866 TTCGAA TT^CGAA
BstEII 1235 GGTCACC G^GT[ACGT]ACC
BstPI 1235 GGTCACC G^GT[ACGT]ACC
BstYI 1147 AGATCC [GA]^GATC[CT]
CelII 2049 GCTGAGC GC^T[ACGT]AGC
EagI 184 CGGCCG C^GGCCG
Eam1105I 213 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 184 CGGCCG C^GGCCG
Eco52I 184 CGGCCG C^GGCCG
EcoNI 2192 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1235 GGTCACC G^GT[ACGT]ACC
EcoRV 1557 GATATC GAT^ATC
FbaI 877 TGATCA T^GATCA
HpaI 2151 GTTAAC GTT^AAC
KasI 17 GGCGCC G^GCGCC
KspI 2016 CCGCGG CCGC^GG
MamI 2792 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1147 AGATCC [GA]^GATC[CT]
MroI 3197 TCCGGA T^CCGGA
NarI 17 GGCGCC GG^CGCC
NdeI 873 CATATG CA^TATG
NspV 2866 TTCGAA TT^CGAA
PacI 3541 TTAATTAA TTAAT^TAA
PaeR7I 3587 CTCGAG C^TCGAG
PflFI 323 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3653 ATTAAT AT^TAAT
RcaI 2179 TCATGA T^CATGA
SacII 2016 CCGCGG CCGC^GG
SapI 1700 GAAGAGC GCTCTTC(1/4)
ScaI 3632 AGTACT AGT^ACT
SfiI 2276 GGCCGCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 17 GGCGCC GGC^GCC
SfuI 2866 TTCGAA TT^CGAA
SmlI 3587 CTCGAG C^T[CT][GA]AG
Tth111I 323 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3587 CTCGAG C^TCGAG
XhoII 1147 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 1790 GACGTC GACGT^C
3083 GACGTC
AccI 1416 GTCGAC GT^[AC][GT]AC
2648 GTCGAC
AlwI 1148 GATCC GGATC(4/5)
2708 GATCC
Aor51HI 654 AGCGCT AGC^GCT
751 AGCGCT
AvaI 3093 CCCGAG C^[CT]CG[GA]G
3587 CTCGAG
BsgI 408 CTGCAC GTGCAG(16/14)
576 GTGCAG
BsiEI 184 CGGCCG CG[GA][CT]^CG
2327 CGACCG
BsmBI 699 GAGACG CGTCTC(1/5)
1275 GAGACG
BsoBI 3093 CCCGAG C^[CT]CG[GA]G
3587 CTCGAG
Bsp1407I 358 TGTACA T^GTACA
3388 TGTACA
BspLU11I 130 ACATGT A^CATGT
3332 ACATGT
BsrFI 586 ACCGGC [GA]^CCGG[CT]
2054 GCCGGT
BsrGI 358 TGTACA T^GTACA
3388 TGTACA
BstXI 1097 CCACCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1954 CCATCAAGATGG
Cfr10I 586 ACCGGC [GA]^CCGG[CT]
2054 GCCGGT
DraII 156 AGGGCCT [GA]G^G[ACGT]CC[CT]
3304 AGGCCCC
Eco47III 654 AGCGCT AGC^GCT
751 AGCGCT
EcoO109I 156 AGGGCCT [GA]G^G[ACGT]CC[CT]
3304 AGGCCCC
HindIII 964 AAGCTT A^AGCTT
1896 AAGCTT
NcoI 508 CCATGG C^CATGG
1822 CCATGG
PshAI 1277 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1763 GACCCTGGTC
SalI 1416 GTCGAC G^TCGAC
2648 GTCGAC
SbfI 854 CCTGCAGG CCTGCA^GG
1187 CCTGCAGG
Sse8387I 854 CCTGCAGG CCTGCA^GG
1187 CCTGCAGG
SspBI 358 TGTACA T^GTACA
3388 TGTACA
Tsp509I 3543 AATT ^AATT
3656 AATT
XcmI 238 CCAGTGACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1822 CCATGGAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I