##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstYI
Bsu36I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I FauI FbaI FseI
FspI HaeII HapII HpaII KasI
KpnI KspI MflI MluI MroI
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NlaIV NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI TaqI Tth111I
TthHB8I Van91I XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AclI 148 AACGTT AA^CGTT
AcyI 695 GGCGTC G[GA]^CG[CT]C
AflII 2580 CTTAAG C^TTAAG
AspHI 911 GAGCAC G[AT]GC[AT]^C
BfrI 2580 CTTAAG C^TTAAG
BmyI 911 GAGCAC G[AGT]GC[ACT]^C
BsaHI 695 GGCGTC G[GA]^CG[CT]C
BsiHKAI 911 GAGCAC G[AT]GC[AT]^C
Bsp1286I 911 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 680 TGTACA T^GTACA
BspMI 1523 ACCTGC ACCTGC(4/8)
BsrGI 680 TGTACA T^GTACA
Bst1107I 2171 GTATAC GTA^TAC
BstXI 1021 CCACAGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2171 GTATAC GTA^TAC
CfoI 144 GCGC GCG^C
EcoNI 2307 CCTTTATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3249 GATATC GAT^ATC
EcoT22I 2071 ATGCAT ATGCA^T
HhaI 144 GCGC GCG^C
Hin1I 695 GGCGTC G[GA]^CG[CT]C
HinP1I 144 GCGC G^CGC
MfeI 209 CAATTG C^AATTG
MunI 209 CAATTG C^AATTG
NdeI 1421 CATATG CA^TATG
NsiI 2071 ATGCAT ATGCA^T
Psp1406I 148 AACGTT AA^CGTT
SexAI 2626 ACCTGGT A^CC[AT]GGT
SpeI 2175 ACTAGT A^CTAGT
SphI 3059 GCATGC GCATG^C
SspBI 680 TGTACA T^GTACA
StuI 2274 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1245 GTAGAC GT^[AC][GT]AC
2171 GTATAC
AseI 1321 ATTAAT AT^TAAT
1950 ATTAAT
AsnI 1321 ATTAAT AT^TAAT
1950 ATTAAT
AvaII 448 GGACC G^G[AT]CC
724 GGTCC
BalI 117 TGGCCA TGG^CCA
1695 TGGCCA
BpmI 422 CTCCAG CTGGAG(16/14)
901 CTCCAG
BsaBI 835 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3706 GATGGTAATC
BsaI 787 GAGACC GGTCTC(1/5)
898 GGTCTC
BspHI 84 TCATGA T^CATGA
2374 TCATGA
EaeI 117 TGGCCA [CT]^GGCC[GA]
1695 TGGCCA
EarI 283 GAAGAG CTCTTC(1/4)
520 GAAGAG
EcoRI 1595 GAATTC G^AATTC
3528 GAATTC
HgaI 648 GCGTC GACGC(5/10)
696 GCGTC
HindIII 2583 AAGCTT A^AGCTT
2986 AAGCTT
Ksp632I 283 GAAGAG CTCTTC(1/4)
520 GAAGAG
MamI 835 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3706 GATGGTAATC
MluNI 117 TGGCCA TGG^CCA
1695 TGGCCA
MscI 117 TGGCCA TGG^CCA
1695 TGGCCA
MspA1I 256 CAGCTG C[AC]G^C[GT]G
904 CAGCTG
MwoI 2236 GCTGATCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2922 GCTTATTTAGC
PshBI 1321 ATTAAT AT^TAAT
1950 ATTAAT
PvuII 256 CAGCTG CAG^CTG
904 CAGCTG
RcaI 84 TCATGA T^CATGA
2374 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI Asp700I BbsI BbvI BfaI
BpuAI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI BspLU11I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI HpaI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI NdeII
NlaIII NspI PleI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XbaI
XmnI