##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AlwNI       
Aor51HI     ApaI        AscI        Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BcgI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmFI       
BspDI       BspEI       BspHI       BspMI       BsrBI       
BssHII      BssSI       BstBI       BstEII      BstPI       
BstUI       CelII       ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       FseI        
FspI        HaeII       Hin1I       HpaI        KasI        
KpnI        KspI        MaeII       MluI        MroI        
MspA1I      MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
Tth111I     Van91I      XbaI        XcmI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI         2485  GTATAC               GT^[AC][GT]AC
AflIII       3258  ACATGT               A^C[GA][CT]GT
AlwI         2892  GATCC                GGATC(4/5)
AvaII          15  GGACC                G^G[AT]CC
BciVI        3957  GTATCC               GTATCC(6/5)
BclI          190  TGATCA               T^GATCA
BglI         2851  GCCATGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2094  AGATCT               A^GATCT
BsgI          937  CTGCAC               GTGCAG(16/14)
BsmBI        2739  CGTCTC               CGTCTC(1/5)
BspLU11I     3258  ACATGT               A^CATGT
Bst1107I     2485  GTATAC               GTA^TAC
BstAPI       3621  GCAGAGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        2094  AGATCT               [GA]^GATC[CT]
BstZ17I      2485  GTATAC               GTA^TAC
Cfr13I         15  GGACC                G^G[ACGT]CC
DraII          14  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI         3649  CCATGG               C^C[GA][CT]GG
EcoO109I       14  AGGACCC              [GA]G^G[ACGT]CC[CT]
FauI         2911  CCCGC                CCCGC(4/6)
FbaI          190  TGATCA               T^GATCA
HgaI            8  GACGC                GACGC(5/10)
HincII         44  GTTGAC               GT[CT]^[GA]AC
HindII         44  GTTGAC               GT[CT]^[GA]AC
MflI         2094  AGATCT               [GA]^GATC[CT]
MslI         3068  CATTAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         3649  CCATGG               C^CATGG
NdeI         2982  CATATG               CA^TATG
NlaIV          15  GGACCC               GG[ACGT]^[ACGT]CC
PaeR7I       2877  CTCGAG               C^TCGAG
PpuMI          14  AGGACCC              [GA]G^G[AT]CC[CT]
Sau96I         15  GGACC                G^G[ACGT]CC
SexAI         151  ACCTGGT              A^CC[AT]GGT
SfaNI        3132  GATGC                GCATC(5/9)
SmaI         3613  CCCGGG               CCC^GGG
StuI         1078  AGGCCT               AGG^CCT
SwaI         3848  ATTTAAAT             ATTT^AAAT
XhoI         2877  CTCGAG               C^TCGAG
XhoII        2094  AGATCT               [GA]^GATC[CT]
XmaCI        3613  CCCGGG               C^CCGGG
XmaI         3613  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

Alw44I        408  GTGCAC               G^TGCAC
             1932  GTGCAC              
AocI         2129  CCTGAGG              CC^T[ACGT]AGG
             3473  CCTGAGG             
ApaLI         408  GTGCAC               G^TGCAC
             1932  GTGCAC              
AseI         1922  ATTAAT               AT^TAAT
             1962  ATTAAT              
AsnI         1922  ATTAAT               AT^TAAT
             1962  ATTAAT              
Asp700I       545  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1117  GAATTCATTC          
AspHI         408  GTGCAC               G[AT]GC[AT]^C
             1932  GTGCAC              
BbsI         2041  GAAGAC               GAAGAC(2/6)
              156  GTCTTC              
BbvCI        2767  CCTCAGC              CCTCAGC(-5/-2)
             3589  GCTGAGG             
BbvI          171  GCTGC                GCAGC(8/12)
             2919  GCTGC               
BpuAI        2041  GAAGAC               GAAGAC(2/6)
              156  GTCTTC              
BsaBI        1921  GATTAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3081  GATGAAAATC          
BsiHKAI       408  GTGCAC               G[AT]GC[AT]^C
             1932  GTGCAC              
BsmI         3747  GAATGC               GAATGC(1/-1)
             3330  GCATTC              
Bsp1407I     1893  TGTACA               T^GTACA
             4005  TGTACA              
BsrFI         751  ACCGGC               [GA]^CCGG[CT]
             1255  GCCGGT              
BsrGI        1893  TGTACA               T^GTACA
             4005  TGTACA              
BstXI        2777  CCAAGTGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3440  CCAGCACTTTGG        
Bsu36I       2129  CCTGAGG              CC^T[ACGT]AGG
             3473  CCTGAGG             
Cfr10I        751  ACCGGC               [GA]^CCGG[CT]
             1255  GCCGGT              
Eco81I       2129  CCTGAGG              CC^T[ACGT]AGG
             3473  CCTGAGG             
EcoT14I       219  CCTTGG               C^C[AT][AT]GG
             3649  CCATGG              
EcoT22I      3075  ATGCAT               ATGCA^T
             3133  ATGCAT              
Fnu4HI        171  GCTGC                GC^[ACGT]GC
             2919  GCTGC               
ItaI          171  GCTGC                GC^[ACGT]GC
             2919  GCTGC               
MamI         1921  GATTAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3081  GATGAAAATC          
MfeI         2136  CAATTG               C^AATTG
             4001  CAATTG              
MunI         2136  CAATTG               C^AATTG
             4001  CAATTG              
MwoI         2851  GCCATGTTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             3621  GCAGAGGTTGC         
NsiI         3075  ATGCAT               ATGCA^T
             3133  ATGCAT              
PshBI        1922  ATTAAT               AT^TAAT
             1962  ATTAAT              
ScaI         3122  AGTACT               AGT^ACT
             3765  AGTACT              
SphI          583  GCATGC               GCATG^C
             3560  GCATGC              
SspBI        1893  TGTACA               T^GTACA
             4005  TGTACA              
StyI          219  CCTTGG               C^C[AT][AT]GG
             3649  CCATGG              
TseI          171  GCTGC                G^C[AT]GC
             2919  GCTGC               
XmnI          545  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1117  GAATTCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaI        BalI        BcnI        BfaI        BmyI        
BpmI        BsaI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        DdeI        DpnII       DraI        EaeI        
EarI        EcoRI       EcoRII      FokI        HaeIII      
HapII       HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MspI        MvaI        NciI        NdeII       
NlaIII      NspI        PleI        RsaI        Sau3AI      
ScrFI       SfcI        SmlI        SspI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     TspRI       
TthHB8I