##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AviII BamHI BanI
BbeI BbrPI BciVI BglI BlpI
Bpu1102I BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BspEI BspMI BsrBI
BsrFI BssHII BssSI CelII Cfr10I
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HaeII Hin1I HincII
HindII HpaI KasI KpnI KspI
MluI MroI MspA1I NaeI NarI
NgoMIV NheI NotI NruI NsiI
PinAI PmaCI PmeI PmlI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SgrAI SnaBI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 2418 GTATAC GT^[AC][GT]AC
AccII 1127 CGCG CG^CG
AflII 2457 CTTAAG C^TTAAG
AlwNI 44 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 2931 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3149 CCTAGG C^CTAGG
BalI 3105 TGGCCA TGG^CCA
BanII 1947 GAGCTC G[GA]GC[CT]^C
BcgI 3830 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2457 CTTAAG C^TTAAG
BlnI 3149 CCTAGG C^CTAGG
BsaBI 1789 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3911 GAGACG CGTCTC(1/5)
BspDI 3222 ATCGAT AT^CGAT
BspLU11I 1860 ACATGT A^CATGT
BsrDI 3779 GCAATG GCAATG(2/0)
Bst1107I 2418 GTATAC GTA^TAC
BstBI 2192 TTCGAA TT^CGAA
BstUI 1127 CGCG CG^CG
BstZ17I 2418 GTATAC GTA^TAC
CfoI 1128 GCGC GCG^C
ClaI 3222 ATCGAT AT^CGAT
DrdI 1226 GACTCACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1084 CCATGG C^C[GA][CT]GG
EaeI 3105 TGGCCA [CT]^GGCC[GA]
FauI 3971 CCCGC CCCGC(4/6)
HapII 991 CCGG C^CGG
HhaI 1128 GCGC GCG^C
HinP1I 1128 GCGC G^CGC
HpaII 991 CCGG C^CGG
MamI 1789 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2247 CAATTG C^AATTG
MluNI 3105 TGGCCA TGG^CCA
MscI 3105 TGGCCA TGG^CCA
MspI 991 CCGG C^CGG
MunI 2247 CAATTG C^AATTG
MvnI 1127 CGCG CG^CG
NcoI 1084 CCATGG C^CATGG
NdeI 9 CATATG CA^TATG
NspV 2192 TTCGAA TT^CGAA
PacI 2585 TTAATTAA TTAAT^TAA
PaeR7I 3850 CTCGAG C^TCGAG
PflFI 2931 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1947 GAGCTC GAGCT^C
SexAI 1209 ACCAGGT A^CC[AT]GGT
SfcI 460 CTACAG C^T[GA][CT]AG
SfuI 2192 TTCGAA TT^CGAA
SmaI 990 CCCGGG CCC^GGG
SpeI 1190 ACTAGT A^CTAGT
SphI 3871 GCATGC GCATG^C
Tth111I 2931 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2014 TCTAGA T^CTAGA
XhoI 3850 CTCGAG C^TCGAG
XmaCI 990 CCCGGG C^CCGGG
XmaI 990 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 1744 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4160 GACTGTGAGTC
AocI 2630 CCTAAGG CC^T[ACGT]AGG
3957 CCTCAGG
Asp700I 65 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3424 GAAAAATTTC
AspEI 1744 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4160 GACTGTGAGTC
AspHI 1947 GAGCTC G[AT]GC[AT]^C
2524 GTGCTC
AvaI 990 CCCGGG C^[CT]CG[GA]G
3850 CTCGAG
BbsI 278 GAAGAC GAAGAC(2/6)
1364 GAAGAC
BbvCI 3014 CCTCAGC CCTCAGC(-5/-2)
3841 CCTCAGC
BclI 142 TGATCA T^GATCA
1074 TGATCA
BcnI 990 CCCGG CC^[GC]GG
991 CCGGG
BpuAI 278 GAAGAC GAAGAC(2/6)
1364 GAAGAC
BsaAI 2215 TACGTG [CT]AC^GT[GA]
3613 TACGTG
BsaI 1707 GAGACC GGTCTC(1/5)
3115 GGTCTC
BsgI 3054 CTGCAC GTGCAG(16/14)
2952 GTGCAG
BsiHKAI 1947 GAGCTC G[AT]GC[AT]^C
2524 GTGCTC
BsmI 392 GAATGC GAATGC(1/-1)
1917 GCATTC
BsoBI 990 CCCGGG C^[CT]CG[GA]G
3850 CTCGAG
BstAPI 1259 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3805 GCAACCTCTGC
Bsu36I 2630 CCTAAGG CC^T[ACGT]AGG
3957 CCTCAGG
Eam1105I 1744 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4160 GACTGTGAGTC
Eco81I 2630 CCTAAGG CC^T[ACGT]AGG
3957 CCTCAGG
FbaI 142 TGATCA T^GATCA
1074 TGATCA
HindIII 548 AAGCTT A^AGCTT
614 AAGCTT
NciI 990 CCCGG CC^[GC]GG
991 CCGGG
PshAI 52 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1521 GACATCAGTC
StuI 3152 AGGCCT AGG^CCT
4141 AGGCCT
XcmI 1101 CCAGTCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2641 CCAAGCAGTGGTTGG
XmnI 65 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3424 GAAAAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AvaII
BbvI BfaI BglII BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspHI BsrGI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoO65I EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshBI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII