##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AclI AflIII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
BbrPI BbsI BcgI BciVI BclI
BlpI Bpu1102I BpuAI BsaBI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
Eam1105I Eco47III EcoRV EcoT22I FbaI
FseI HpaI MamI MluI NdeI
NotI NruI NsiI NspI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 2809 GGTACC G^GTACC
AccI 1957 GTAGAC GT^[AC][GT]AC
AflII 2907 CTTAAG C^TTAAG
Alw44I 1548 GTGCAC G^TGCAC
ApaLI 1548 GTGCAC G^TGCAC
Asp700I 1556 GAACCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2809 GGTACC G^GTACC
AspI 1723 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3094 TGCGCA TGC^GCA
BamHI 2630 GGATCC G^GATCC
BfrI 2907 CTTAAG C^TTAAG
BglI 576 GCCTCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2942 CACGTA [CT]AC^GT[GA]
BsgI 871 CTGCAC GTGCAG(16/14)
BsrDI 1018 CATTGC GCAATG(2/0)
BstAPI 380 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3133 GGTGACC G^GT[ACGT]ACC
BstPI 3133 GGTGACC G^GT[ACGT]ACC
DrdI 2 GACAAAGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 339 CGGCCG C^GGCCG
EclXI 339 CGGCCG C^GGCCG
Eco52I 339 CGGCCG C^GGCCG
EcoO65I 3133 GGTGACC G^GT[ACGT]ACC
EcoRI 3708 GAATTC G^AATTC
FspI 3094 TGCGCA TGC^GCA
KpnI 2809 GGTACC GGTAC^C
KspI 192 CCGCGG CCGC^GG
PflFI 1723 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 192 CCGCGG CCGC^GG
Tth111I 1723 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 734 TCTAGA T^CTAGA
XmnI 1556 GAACCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 111 TCCGGA T^CCGGA
1030 TCCGGA
AcsI 3633 AAATTC [GA]^AATT[CT]
3708 GAATTC
AlwNI 3214 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
3595 CAGCTCCTG
ApoI 3633 AAATTC [GA]^AATT[CT]
3708 GAATTC
AvrII 3363 CCTAGG C^CTAGG
3571 CCTAGG
BalI 1297 TGGCCA TGG^CCA
2139 TGGCCA
BbeI 392 GGCGCC GGCGC^C
1336 GGCGCC
BbvCI 855 GCTGAGG CCTCAGC(-5/-2)
2081 GCTGAGG
BglII 2827 AGATCT A^GATCT
2911 AGATCT
BlnI 3363 CCTAGG C^CTAGG
3571 CCTAGG
BsaWI 111 TCCGGA [AT]^CCGG[AT]
1030 TCCGGA
BseAI 111 TCCGGA T^CCGGA
1030 TCCGGA
BsiEI 9 CGGTCG CG[GA][CT]^CG
339 CGGCCG
BspEI 111 TCCGGA T^CCGGA
1030 TCCGGA
BspHI 359 TCATGA T^CATGA
2638 TCATGA
BspMI 3435 ACCTGC ACCTGC(4/8)
3656 ACCTGC
BstXI 499 CCAGGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2618 CCAACCTCCTGG
HincII 205 GTTGAC GT[CT]^[GA]AC
2715 GTCAAC
HindII 205 GTTGAC GT[CT]^[GA]AC
2715 GTCAAC
HindIII 1443 AAGCTT A^AGCTT
2385 AAGCTT
KasI 392 GGCGCC G^GCGCC
1336 GGCGCC
MaeII 2943 ACGT A^CGT
3349 ACGT
MluNI 1297 TGGCCA TGG^CCA
2139 TGGCCA
MroI 111 TCCGGA T^CCGGA
1030 TCCGGA
MscI 1297 TGGCCA TGG^CCA
2139 TGGCCA
MslI 1861 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3739 CATTGGAGTG
NarI 392 GGCGCC GG^CGCC
1336 GGCGCC
NcoI 1294 CCATGG C^CATGG
3444 CCATGG
NheI 1328 GCTAGC G^CTAGC
1619 GCTAGC
PaeR7I 1921 CTCGAG C^TCGAG
2317 CTCGAG
PvuII 1323 CAGCTG CAG^CTG
2864 CAGCTG
RcaI 359 TCATGA T^CATGA
2638 TCATGA
SapI 1611 GAAGAGC GCTCTTC(1/4)
3054 GAAGAGC
SfoI 392 GGCGCC GGC^GCC
1336 GGCGCC
XhoI 1921 CTCGAG C^TCGAG
2317 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MfeI MflI
MnlI MseI MspA1I MspI MunI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI