##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI ApoI AscI
AseI AsnI AspEI AvrII BbrPI
BbsI BciVI BclI BfrI BlnI
BpuAI BsaAI BseAI BsiWI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoRI EcoRV EcoT22I
FbaI HindIII HpaI KspI MfeI
MluI MroI MseI MunI NheI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspI SwaI
Tru9I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AccI 614 GTCGAC GT^[AC][GT]AC
AocI 2339 CCTGAGG CC^T[ACGT]AGG
Asp700I 1912 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 321 GCAAGTACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 841 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1331 GAGACG CGTCTC(1/5)
BsmI 274 GAATGC GAATGC(1/-1)
BstEII 1885 GGTCACC G^GT[ACGT]ACC
BstPI 1885 GGTCACC G^GT[ACGT]ACC
Bsu36I 2339 CCTGAGG CC^T[ACGT]AGG
DraIII 3362 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2339 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1885 GGTCACC G^GT[ACGT]ACC
FseI 2670 GGCCGGCC GGCCGG^CC
MamI 841 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 3264 CATATG CA^TATG
NotI 4258 GCGGCCGC GC^GGCCGC
SacI 3681 GAGCTC GAGCT^C
SalI 614 GTCGAC G^TCGAC
SapI 709 GAAGAGC GCTCTTC(1/4)
SbfI 2888 CCTGCAGG CCTGCA^GG
Sse8387I 2888 CCTGCAGG CCTGCA^GG
TfiI 1310 GATTC G^A[AT]TC
XmnI 1912 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Aor51HI 481 AGCGCT AGC^GCT
4146 AGCGCT
BsaWI 2599 ACCGGA [AT]^CCGG[AT]
3400 TCCGGT
BsiEI 3572 CGGCCG CG[GA][CT]^CG
4259 CGGCCG
Bsp1407I 1227 TGTACA T^GTACA
1429 TGTACA
BsrDI 3751 CATTGC GCAATG(2/0)
2549 GCAATG
BsrGI 1227 TGTACA T^GTACA
1429 TGTACA
EagI 3572 CGGCCG C^GGCCG
4259 CGGCCG
EclXI 3572 CGGCCG C^GGCCG
4259 CGGCCG
Eco47III 481 AGCGCT AGC^GCT
4146 AGCGCT
Eco52I 3572 CGGCCG C^GGCCG
4259 CGGCCG
HincII 614 GTCGAC GT[CT]^[GA]AC
647 GTCAAC
HindII 614 GTCGAC GT[CT]^[GA]AC
647 GTCAAC
NspI 132 GCATGC [GA]CATG^[CT]
764 GCATGC
PleI 3084 GACTC GAGTC(4/5)
352 GAGTC
SexAI 1716 ACCAGGT A^CC[AT]GGT
3363 ACCTGGT
SphI 132 GCATGC GCATG^C
764 GCATGC
SspBI 1227 TGTACA T^GTACA
1429 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI Asp718I AspHI AspI AvaI
AvaII AviII BalI BamHI BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflFI PflMI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII