##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AciI AclI
AgeI AhdI AlwNI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BcgI BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspMI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI FauI FseI
FspI HpaI KpnI KspI MamI
MluI MroI MspA1I NaeI NcoI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 982 GTCTAC GT^[AC][GT]AC
AccII 1096 CGCG CG^CG
AcyI 1918 GGCGCC G[GA]^CG[CT]C
Alw44I 2785 GTGCAC G^TGCAC
AocI 3780 CCTGAGG CC^T[ACGT]AGG
ApaLI 2785 GTGCAC G^TGCAC
Asp700I 1518 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 70 GAGCTC G[GA]GC[CT]^C
BbeI 1918 GGCGCC GGCGC^C
BbrPI 1114 CACGTG CAC^GTG
BbvCI 2014 CCTCAGC CCTCAGC(-5/-2)
BcnI 254 CCGGG CC^[GC]GG
BglI 3048 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 287 AGATCT A^GATCT
BsaHI 1918 GGCGCC G[GA]^CG[CT]C
BsaI 2841 GGTCTC GGTCTC(1/5)
BsiYI 2505 CCAGCCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 2505 CCAGCCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 3717 TCATGA T^CATGA
BspLU11I 1567 ACATGT A^CATGT
BsrDI 2756 CATTGC GCAATG(2/0)
BssSI 2367 CTCGTG CACGAG(-5/-1)
BstEII 3813 GGTAACC G^GT[ACGT]ACC
BstPI 3813 GGTAACC G^GT[ACGT]ACC
BstUI 1096 CGCG CG^CG
BstXI 1137 CCACAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3780 CCTGAGG CC^T[ACGT]AGG
Eco81I 3780 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3813 GGTAACC G^GT[ACGT]ACC
EcoRV 2120 GATATC GAT^ATC
EcoT22I 3762 ATGCAT ATGCA^T
HaeII 1918 GGCGCC [GA]GCGC^[CT]
Hin1I 1918 GGCGCC G[GA]^CG[CT]C
HincII 3032 GTTGAC GT[CT]^[GA]AC
HindII 3032 GTTGAC GT[CT]^[GA]AC
KasI 1918 GGCGCC G^GCGCC
MvnI 1096 CGCG CG^CG
NarI 1918 GGCGCC GG^CGCC
NciI 254 CCGGG CC^[GC]GG
NheI 4444 GCTAGC G^CTAGC
NsiI 3762 ATGCAT ATGCA^T
PflMI 2505 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1114 CACGTG CAC^GTG
PmlI 1114 CACGTG CAC^GTG
PstI 385 CTGCAG CTGCA^G
RcaI 3717 TCATGA T^CATGA
SacI 70 GAGCTC GAGCT^C
SexAI 2837 ACCTGGT A^CC[AT]GGT
SfoI 1918 GGCGCC GGC^GCC
SpeI 4116 ACTAGT A^CTAGT
StuI 4301 AGGCCT AGG^CCT
Van91I 2505 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 925 CCAATGTGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1518 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AflII 3494 CTTAAG C^TTAAG
4184 CTTAAG
AseI 1155 ATTAAT AT^TAAT
1869 ATTAAT
AsnI 1155 ATTAAT AT^TAAT
1869 ATTAAT
BalI 301 TGGCCA TGG^CCA
3055 TGGCCA
BbsI 472 GAAGAC GAAGAC(2/6)
2196 GAAGAC
BciVI 846 GGATAC GTATCC(6/5)
1335 GGATAC
BclI 182 TGATCA T^GATCA
1036 TGATCA
BfrI 3494 CTTAAG C^TTAAG
4184 CTTAAG
BpuAI 472 GAAGAC GAAGAC(2/6)
2196 GAAGAC
BsaAI 1114 CACGTG [CT]AC^GT[GA]
2075 TACGTG
BseRI 2260 CTCCTC GAGGAG(10/8)
948 GAGGAG
Bsp1407I 972 TGTACA T^GTACA
4194 TGTACA
BsrGI 972 TGTACA T^GTACA
4194 TGTACA
BstYI 287 AGATCT [GA]^GATC[CT]
2588 AGATCC
CfoI 1095 GCGC GCG^C
1919 GCGC
EaeI 301 TGGCCA [CT]^GGCC[GA]
3055 TGGCCA
EarI 1708 CTCTTC CTCTTC(1/4)
3881 GAAGAG
FbaI 182 TGATCA T^GATCA
1036 TGATCA
HapII 254 CCGG C^CGG
1928 CCGG
HhaI 1095 GCGC GCG^C
1919 GCGC
HinP1I 1095 GCGC G^CGC
1919 GCGC
HpaII 254 CCGG C^CGG
1928 CCGG
Ksp632I 1708 CTCTTC CTCTTC(1/4)
3881 GAAGAG
MaeII 1115 ACGT A^CGT
2076 ACGT
MflI 287 AGATCT [GA]^GATC[CT]
2588 AGATCC
MluNI 301 TGGCCA TGG^CCA
3055 TGGCCA
MscI 301 TGGCCA TGG^CCA
3055 TGGCCA
MspI 254 CCGG C^CGG
1928 CCGG
NspI 978 GCATGT [GA]CATG^[CT]
1567 ACATGT
PpuMI 2029 GGGTCCT [GA]G^G[AT]CC[CT]
2134 GGGACCC
PshAI 2136 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4215 GACAGTTGTC
PshBI 1155 ATTAAT AT^TAAT
1869 ATTAAT
ScaI 3150 AGTACT AGT^ACT
4042 AGTACT
SspBI 972 TGTACA T^GTACA
4194 TGTACA
SspI 3722 AATATT AAT^ATT
4319 AATATT
SwaI 3501 ATTTAAAT ATTT^AAAT
3851 ATTTAAAT
TaqI 821 TCGA T^CGA
4241 TCGA
TthHB8I 821 TCGA T^CGA
4241 TCGA
XhoII 287 AGATCT [GA]^GATC[CT]
2588 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AspHI AvaII BanI BbvI
BfaI BmyI BpmI BsaJI BsiHKAI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI DraII EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HindIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MfeI MnlI MseI MslI MunI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI