##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AccIII      AclI        AflII       AflIII      AgeI        
Alw44I      AlwNI       ApaI        ApaLI       AscI        
AspI        AviII       BalI        BfrI        BglII       
BsaBI       BseAI       BsgI        BsiWI       BsmBI       
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BstAPI      BstBI       ClaI        CpoI        EagI        
EclXI       Eco52I      EcoRV       FseI        FspI        
MamI        MluI        MluNI       MroI        MscI        
NheI        NotI        NruI        NspV        PacI        
PflFI       PflMI       PinAI       PmeI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SbfI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
Van91I      XbaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AatII        1839  GACGTC               GACGT^C
Acc65I        244  GGTACC               G^GTACC
AhdI          900  GACCTATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          943  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       207  AGCGCT               AGC^GCT
AseI         3585  ATTAAT               AT^TAAT
AsnI         3585  ATTAAT               AT^TAAT
Asp718I       244  GGTACC               G^GTACC
AspEI         900  GACCTATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        3623  CCTAGG               C^CTAGG
BamHI         195  GGATCC               G^GATCC
BbrPI         736  CACGTG               CAC^GTG
BbsI         1213  GTCTTC               GAAGAC(2/6)
BbvCI         565  GCTGAGG              CCTCAGC(-5/-2)
BcgI          386  CGACGCAGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1536  TGATCA               T^GATCA
BlnI         3623  CCTAGG               C^CTAGG
BpuAI        1213  GTCTTC               GAAGAC(2/6)
BsaAI         736  CACGTG               [CT]AC^GT[GA]
BsaI          898  GAGACC               GGTCTC(1/5)
BsiEI         529  CGGTCG               CG[GA][CT]^CG
BsrDI        2706  CATTGC               GCAATG(2/0)
BsrFI         378  GCCGGC               [GA]^CCGG[CT]
BssHII        521  GCGCGC               G^CGCGC
BssSI         249  CACGAG               CACGAG(-5/-1)
Bst1107I     3775  GTATAC               GTA^TAC
BstXI        3273  CCATGACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      3775  GTATAC               GTA^TAC
Bsu36I        943  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        378  GCCGGC               [GA]^CCGG[CT]
DraIII         11  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          357  GACAACAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          821  TGGCCG               [CT]^GGCC[GA]
Eam1105I      900  GACCTATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      207  AGCGCT               AGC^GCT
Eco81I        943  CCTGAGG              CC^T[ACGT]AGG
EcoNI         750  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1536  TGATCA               T^GATCA
HpaI         3884  GTTAAC               GTT^AAC
KpnI          244  GGTACC               GGTAC^C
MfeI         3317  CAATTG               C^AATTG
MunI         3317  CAATTG               C^AATTG
NaeI          378  GCCGGC               GCC^GGC
NdeI         2209  CATATG               CA^TATG
NgoMIV        378  GCCGGC               G^CCGGC
PaeR7I        201  CTCGAG               C^TCGAG
PmaCI         736  CACGTG               CAC^GTG
PmlI          736  CACGTG               CAC^GTG
PshBI        3585  ATTAAT               AT^TAAT
PstI         1805  CTGCAG               CTGCA^G
SalI          531  GTCGAC               G^TCGAC
SapI          973  GAAGAGC              GCTCTTC(1/4)
SfiI          282  GGCCCGCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          232  CCCGGG               CCC^GGG
StuI          306  AGGCCT               AGG^CCT
TfiI         1935  GAATC                G^A[AT]TC
XhoI          201  CTCGAG               C^TCGAG
XmaCI         232  CCCGGG               C^CCGGG
XmaI          232  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BbeI          254  GGCGCC               GGCGC^C
              460  GGCGCC              
BciVI        3801  GGATAC               GTATCC(6/5)
              723  GTATCC              
BfaI         3121  CTAG                 C^TAG
             3624  CTAG                
BglI          283  GCCCGCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              700  GCCTCCTGGGC         
BlpI         1940  GCTCAGC              GC^T[ACGT]AGC
             2444  GCTCAGC             
Bpu1102I     1940  GCTCAGC              GC^T[ACGT]AGC
             2444  GCTCAGC             
BsaWI         527  TCCGGT               [AT]^CCGG[AT]
             2714  ACCGGA              
CelII        1940  GCTCAGC              GC^T[ACGT]AGC
             2444  GCTCAGC             
DraI         2239  TTTAAA               TTT^AAA
             3902  TTTAAA              
EcoT22I      2218  ATGCAT               ATGCA^T
             2890  ATGCAT              
HindIII      2261  AAGCTT               A^AGCTT
             3171  AAGCTT              
KasI          254  GGCGCC               G^GCGCC
              460  GGCGCC              
MaeI         3121  CTAG                 C^TAG
             3624  CTAG                
NarI          254  GGCGCC               GG^CGCC
              460  GGCGCC              
NsiI         2218  ATGCAT               ATGCA^T
             2890  ATGCAT              
NspI          997  GCATGT               [GA]CATG^[CT]
             1347  ACATGC              
ScaI         1230  AGTACT               AGT^ACT
             3406  AGTACT              
SfoI          254  GGCGCC               GGC^GCC
              460  GGCGCC              
SmlI          201  CTCGAG               C^T[CT][GA]AG
             3265  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        AlwI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspMI       BsrGI       BsrI        BssKI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EarI        EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     KspI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PvuII       RsaI        SacI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SspBI       StyI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       XmnI