##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Aor51HI ApaI AscI Asp700I
BbeI BbrPI BcgI BglI BsaAI
BsaHI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I ClaI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I FseI HaeII
HgaI Hin1I HpaI KasI KspI
MfeI MluI MroI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
SacII SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 193 GGTACC G^GTACC
AhdI 895 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2582 GTGCAC G^TGCAC
AlwNI 1504 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2582 GTGCAC G^TGCAC
Asp718I 193 GGTACC G^GTACC
AspEI 895 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2044 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 47 CTCGAG C^[CT]CG[GA]G
AvrII 963 CCTAGG C^CTAGG
BamHI 1723 GGATCC G^GATCC
BanII 553 GAGCTC G[GA]GC[CT]^C
BbvCI 878 CCTCAGC CCTCAGC(-5/-2)
BciVI 1339 GGATAC GTATCC(6/5)
BclI 744 TGATCA T^GATCA
BlnI 963 CCTAGG C^CTAGG
BsaBI 3242 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 248 GAGACC GGTCTC(1/5)
BsaWI 316 ACCGGA [AT]^CCGG[AT]
BsgI 2677 GTGCAG GTGCAG(16/14)
BsmBI 2816 GAGACG CGTCTC(1/5)
BsmI 133 GCATTC GAATGC(1/-1)
BsoBI 47 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1649 TGTACA T^GTACA
BspHI 2092 TCATGA T^CATGA
BspMI 2875 ACCTGC ACCTGC(4/8)
BsrGI 1649 TGTACA T^GTACA
BstAPI 133 GCATTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 313 CGGACCG CG^G[AT]CCG
DraIII 15 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1390 GACTTCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1236 CCGTGG C^C[GA][CT]GG
Eam1105I 895 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3553 GAATTC G^AATTC
EcoT22I 3067 ATGCAT ATGCA^T
FbaI 744 TGATCA T^GATCA
HincII 332 GTCGAC GT[CT]^[GA]AC
HindII 332 GTCGAC GT[CT]^[GA]AC
KpnI 193 GGTACC GGTAC^C
MaeII 145 ACGT A^CGT
MamI 3242 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 3788 CACCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 3846 GCCGGC GCC^GGC
NgoMIV 3846 GCCGGC G^CCGGC
NsiI 3067 ATGCAT ATGCA^T
PaeR7I 47 CTCGAG C^TCGAG
PflFI 2044 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2092 TCATGA T^CATGA
RsrII 313 CGGACCG CG^G[AT]CCG
SacI 553 GAGCTC GAGCT^C
SalI 332 GTCGAC G^TCGAC
SexAI 3378 ACCTGGT A^CC[AT]GGT
SspBI 1649 TGTACA T^GTACA
StuI 705 AGGCCT AGG^CCT
Tth111I 2044 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 47 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 1563 CTTAAG C^TTAAG
3121 CTTAAG
AflIII 58 ACATGT A^C[GA][CT]GT
2054 ACATGT
AseI 1514 ATTAAT AT^TAAT
3305 ATTAAT
AsnI 1514 ATTAAT AT^TAAT
3305 ATTAAT
AviII 710 TGCGCA TGC^GCA
3424 TGCGCA
BcnI 2925 CCCGG CC^[GC]GG
3369 CCGGG
BfrI 1563 CTTAAG C^TTAAG
3121 CTTAAG
BglII 285 AGATCT A^GATCT
1292 AGATCT
BspLU11I 58 ACATGT A^CATGT
2054 ACATGT
DraI 168 TTTAAA TTT^AAA
4068 TTTAAA
EcoRV 52 GATATC GAT^ATC
955 GATATC
FauI 2772 CCCGC CCCGC(4/6)
453 GCGGG
FspI 710 TGCGCA TGC^GCA
3424 TGCGCA
NciI 2925 CCCGG CC^[GC]GG
3369 CCGGG
PflMI 827 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3329 CCAAAACTTGG
PpuMI 3271 GGGACCT [GA]G^G[AT]CC[CT]
3456 AGGACCT
PshBI 1514 ATTAAT AT^TAAT
3305 ATTAAT
SapI 511 GAAGAGC GCTCTTC(1/4)
4034 GAAGAGC
SphI 2133 GCATGC GCATG^C
3065 GCATGC
Van91I 827 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3329 CCAAAACTTGG
XbaI 2968 TCTAGA T^CTAGA
3207 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AvaII
BalI BanI BbsI BbvI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII