##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AflII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI BbrPI BclI BfrI
BsaBI BsaI BsiWI BspHI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CpoI DraI Eam1105I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI MamI MluI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmlI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1321 GACGTC GACGT^C
Acc65I 2674 GGTACC G^GTACC
AocI 2005 CCTGAGG CC^T[ACGT]AGG
Asp718I 2674 GGTACC G^GTACC
AviII 862 TGCGCA TGC^GCA
AvrII 2691 CCTAGG C^CTAGG
BamHI 2775 GGATCC G^GATCC
BbeI 1864 GGCGCC GGCGC^C
BbvCI 685 CCTCAGC CCTCAGC(-5/-2)
BciVI 1194 GTATCC GTATCC(6/5)
BglI 2124 GCCCTATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1510 AGATCT A^GATCT
BlnI 2691 CCTAGG C^CTAGG
BsmBI 426 CGTCTC CGTCTC(1/5)
Bsp1407I 668 TGTACA T^GTACA
BspDI 208 ATCGAT AT^CGAT
BspLU11I 665 ACATGT A^CATGT
BsrGI 668 TGTACA T^GTACA
BstXI 1406 CCACGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2005 CCTGAGG CC^T[ACGT]AGG
ClaI 208 ATCGAT AT^CGAT
DrdI 2419 GACAAGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2005 CCTGAGG CC^T[ACGT]AGG
FspI 862 TGCGCA TGC^GCA
KasI 1864 GGCGCC G^GCGCC
KpnI 2674 GGTACC GGTAC^C
KspI 1169 CCGCGG CCGC^GG
NaeI 2547 GCCGGC GCC^GGC
NarI 1864 GGCGCC GG^CGCC
NgoMIV 2547 GCCGGC G^CCGGC
PshAI 2403 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 54 CAGCTG CAG^CTG
SacI 1773 GAGCTC GAGCT^C
SacII 1169 CCGCGG CCGC^GG
SexAI 3381 ACCTGGT A^CC[AT]GGT
SfoI 1864 GGCGCC GGC^GCC
SnaBI 2431 TACGTA TAC^GTA
SphI 1242 GCATGC GCATG^C
SspBI 668 TGTACA T^GTACA
XcmI 201 CCATAGGATCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 1170 CGCG CG^CG
1652 CGCG
AclI 46 AACGTT AA^CGTT
1684 AACGTT
AcsI 3032 GAATTT [GA]^AATT[CT]
3426 AAATTT
AfaI 669 GTAC GT^AC
2675 GTAC
AflIII 665 ACATGT A^C[GA][CT]GT
2477 ACGTGT
AlwNI 709 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
2000 CAGCCCCTG
ApoI 3032 GAATTT [GA]^AATT[CT]
3426 AAATTT
BalI 2511 TGGCCA TGG^CCA
3157 TGGCCA
BcgI 2739 CGATGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2964 CGAACTGTATGC
BlpI 2521 GCTGAGC GC^T[ACGT]AGC
2988 GCTCAGC
Bpu1102I 2521 GCTGAGC GC^T[ACGT]AGC
2988 GCTCAGC
BsaAI 2431 TACGTA [CT]AC^GT[GA]
2932 TACGTG
BsgI 729 CTGCAC GTGCAG(16/14)
1706 GTGCAG
BsiEI 1030 CGGCCG CG[GA][CT]^CG
2142 CGGCCG
BsmI 3365 GAATGC GAATGC(1/-1)
1951 GCATTC
BsrFI 2140 ACCGGC [GA]^CCGG[CT]
2547 GCCGGC
BstEII 978 GGTCACC G^GT[ACGT]ACC
2938 GGTGACC
BstPI 978 GGTCACC G^GT[ACGT]ACC
2938 GGTGACC
BstUI 1170 CGCG CG^CG
1652 CGCG
CelII 2521 GCTGAGC GC^T[ACGT]AGC
2988 GCTCAGC
Cfr10I 2140 ACCGGC [GA]^CCGG[CT]
2547 GCCGGC
DraIII 175 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
2807 CACCAAGTG
EagI 1030 CGGCCG C^GGCCG
2142 CGGCCG
EarI 157 GAAGAG CTCTTC(1/4)
1570 GAAGAG
EclXI 1030 CGGCCG C^GGCCG
2142 CGGCCG
Eco52I 1030 CGGCCG C^GGCCG
2142 CGGCCG
EcoNI 1973 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2580 CCTGAGAGAGG
EcoO65I 978 GGTCACC G^GT[ACGT]ACC
2938 GGTGACC
HgaI 127 GACGC GACGC(5/10)
893 GCGTC
HindIII 2422 AAGCTT A^AGCTT
3127 AAGCTT
Ksp632I 157 GAAGAG CTCTTC(1/4)
1570 GAAGAG
MfeI 3241 CAATTG C^AATTG
3308 CAATTG
MluNI 2511 TGGCCA TGG^CCA
3157 TGGCCA
MscI 2511 TGGCCA TGG^CCA
3157 TGGCCA
MunI 3241 CAATTG C^AATTG
3308 CAATTG
MvnI 1170 CGCG CG^CG
1652 CGCG
Psp1406I 46 AACGTT AA^CGTT
1684 AACGTT
PstI 1067 CTGCAG CTGCA^G
2554 CTGCAG
RsaI 669 GTAC GT^AC
2675 GTAC
StuI 526 AGGCCT AGG^CCT
674 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AccIII AciI AcyI AluI
AlwI Aor51HI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BseAI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eco47III EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MroI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI