##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AspEI AviII AvrII BamHI
BanII BbeI BbrPI BcgI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BsrBI BssHII BssSI
BstAPI BstBI CelII CfoI ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HpaI KasI KspI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SpeI Sse8387I SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 391 GGTACC G^GTACC
AccII 2178 CGCG CG^CG
AflIII 355 ACACGT A^C[GA][CT]GT
AseI 1220 ATTAAT AT^TAAT
AsnI 1220 ATTAAT AT^TAAT
Asp700I 1480 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 391 GGTACC G^GTACC
AspHI 2769 GAGCAC G[AT]GC[AT]^C
AspI 1428 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 81 CCCGAG C^[CT]CG[GA]G
BalI 3401 TGGCCA TGG^CCA
BbvCI 31 CCTCAGC CCTCAGC(-5/-2)
BcnI 1535 CCGGG CC^[GC]GG
BmyI 2769 GAGCAC G[AGT]GC[ACT]^C
BsaAI 3031 TACGTA [CT]AC^GT[GA]
BsaBI 792 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2713 TCCGGT [AT]^CCGG[AT]
BsiHKAI 2769 GAGCAC G[AT]GC[AT]^C
BsoBI 81 CCCGAG C^[CT]CG[GA]G
Bsp1286I 2769 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 2467 TGTACA T^GTACA
BsrFI 1448 GCCGGT [GA]^CCGG[CT]
BsrGI 2467 TGTACA T^GTACA
Bst1107I 875 GTATAC GTA^TAC
BstEII 99 GGTAACC G^GT[ACGT]ACC
BstPI 99 GGTAACC G^GT[ACGT]ACC
BstUI 2178 CGCG CG^CG
BstZ17I 875 GTATAC GTA^TAC
Cfr10I 1448 GCCGGT [GA]^CCGG[CT]
DrdI 3069 GACAACAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2440 CCATGG C^C[GA][CT]GG
EcoO65I 99 GGTAACC G^GT[ACGT]ACC
EcoRI 2154 GAATTC G^AATTC
EcoT22I 265 ATGCAT ATGCA^T
FauI 719 CCCGC CCCGC(4/6)
HindIII 2623 AAGCTT A^AGCTT
KpnI 391 GGTACC GGTAC^C
MamI 792 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3401 TGGCCA TGG^CCA
MscI 3401 TGGCCA TGG^CCA
MvnI 2178 CGCG CG^CG
NciI 1535 CCGGG CC^[GC]GG
NcoI 2440 CCATGG C^CATGG
NsiI 265 ATGCAT ATGCA^T
PflFI 1428 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2831 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1220 ATTAAT AT^TAAT
PstI 2955 CTGCAG CTGCA^G
PvuII 2272 CAGCTG CAG^CTG
SexAI 3359 ACCTGGT A^CC[AT]GGT
SnaBI 3031 TACGTA TAC^GTA
SphI 3596 GCATGC GCATG^C
SspBI 2467 TGTACA T^GTACA
SspI 1023 AATATT AAT^ATT
StuI 69 AGGCCT AGG^CCT
Tth111I 1428 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1480 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 337 GACGCC G[GA]^CG[CT]C
769 GGCGTC
AlwNI 1929 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2830 CAGGTCCTG
BciVI 239 GGATAC GTATCC(6/5)
2162 GTATCC
BsaHI 337 GACGCC G[GA]^CG[CT]C
769 GGCGTC
BsaI 1056 GAGACC GGTCTC(1/5)
1833 GAGACC
BsgI 435 CTGCAC GTGCAG(16/14)
1255 CTGCAC
BsmBI 2180 CGTCTC CGTCTC(1/5)
1116 GAGACG
BsmFI 350 GGGAC GGGAC(10/14)
2509 GGGAC
BspHI 917 TCATGA T^CATGA
3619 TCATGA
BstXI 2425 CCAACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2977 CCATCTATGTGG
BstYI 621 GGATCT [GA]^GATC[CT]
2192 GGATCT
EaeI 2244 TGGCCG [CT]^GGCC[GA]
3401 TGGCCA
Hin1I 337 GACGCC G[GA]^CG[CT]C
769 GGCGTC
MaeII 357 ACGT A^CGT
3032 ACGT
MflI 621 GGATCT [GA]^GATC[CT]
2192 GGATCT
MspA1I 2031 CCGCTG C[AC]G^C[GT]G
2272 CAGCTG
PflMI 1470 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3393 CCATCCAATGG
PshAI 765 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3318 GACTGCTGTC
RcaI 917 TCATGA T^CATGA
3619 TCATGA
TaqI 3479 TCGA T^CGA
3542 TCGA
TthHB8I 3479 TCGA T^CGA
3542 TCGA
Van91I 1470 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3393 CCATCCAATGG
XcmI 1957 CCACTATTTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3389 CCAGCCATCCAATGG
XhoII 621 GGATCT [GA]^GATC[CT]
2192 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI AvaII BanI
BbsI BbvI BfaI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmI BspMI BsrDI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI