##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvaI AviII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BcnI BglI BlpI Bpu1102I BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspHI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstPI BstUI
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaI HpaII KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SphI Sse8387I Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2613 GTATAC GT^[AC][GT]AC
AflII 3070 CTTAAG C^TTAAG
Alw44I 1966 GTGCAC G^TGCAC
AlwI 3217 GGATC GGATC(4/5)
ApaLI 1966 GTGCAC G^TGCAC
AspHI 1966 GTGCAC G[AT]GC[AT]^C
AvaII 1892 GGACC G^G[AT]CC
AvrII 3009 CCTAGG C^CTAGG
BanII 295 GAGCCC G[GA]GC[CT]^C
BciVI 3024 GTATCC GTATCC(6/5)
BclI 3126 TGATCA T^GATCA
BfrI 3070 CTTAAG C^TTAAG
BlnI 3009 CCTAGG C^CTAGG
BsaAI 3018 TACGTA [CT]AC^GT[GA]
BsaI 3917 GGTCTC GGTCTC(1/5)
BsaJI 3009 CCTAGG C^C[ACGT][ACGT]GG
BsiHKAI 1966 GTGCAC G[AT]GC[AT]^C
BspMI 303 ACCTGC ACCTGC(4/8)
Bst1107I 2613 GTATAC GTA^TAC
BstAPI 9 GCAAACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 59 CCAGCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2613 GTATAC GTA^TAC
DrdI 3357 GACCCAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2192 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 3009 CCTAGG C^C[AT][AT]GG
FauI 1091 GCGGG CCCGC(4/6)
FbaI 3126 TGATCA T^GATCA
HincII 2537 GTCAAC GT[CT]^[GA]AC
HindII 2537 GTCAAC GT[CT]^[GA]AC
HindIII 1097 AAGCTT A^AGCTT
MaeII 3019 ACGT A^CGT
MspA1I 60 CAGCTG C[AC]G^C[GT]G
PflMI 3532 CCATATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1891 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 60 CAGCTG CAG^CTG
SapI 565 GAAGAGC GCTCTTC(1/4)
ScaI 3443 AGTACT AGT^ACT
SnaBI 3018 TACGTA TAC^GTA
SpeI 505 ACTAGT A^CTAGT
StyI 3009 CCTAGG C^C[AT][AT]GG
TaqI 2458 TCGA T^CGA
TthHB8I 2458 TCGA T^CGA
Van91I 3532 CCATATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 1443 CCGC CCGC(-3/-1)
1091 GCGG
AflIII 2124 ACATGT A^C[GA][CT]GT
3430 ACATGT
BbsI 495 GAAGAC GAAGAC(2/6)
1218 GAAGAC
BglII 2995 AGATCT A^GATCT
3236 AGATCT
BpuAI 495 GAAGAC GAAGAC(2/6)
1218 GAAGAC
BspLU11I 2124 ACATGT A^CATGT
3430 ACATGT
Cfr13I 1892 GGACC G^G[ACGT]CC
2330 GGCCC
DraII 1891 AGGACCC [GA]G^G[ACGT]CC[CT]
2329 AGGCCCT
EcoO109I 1891 AGGACCC [GA]G^G[ACGT]CC[CT]
2329 AGGCCCT
NheI 3245 GCTAGC G^CTAGC
3582 GCTAGC
NlaIV 294 GGAGCC GG[ACGT]^[ACGT]CC
1892 GGACCC
Sau96I 1892 GGACC G^G[ACGT]CC
2330 GGCCC
SfcI 1353 CTATAG C^T[GA][CT]AG
3119 CTGTAG
SwaI 614 ATTTAAAT ATTT^AAAT
2082 ATTTAAAT
Tsp45I 2128 GTCAC ^GT[GC]AC
3523 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI Asp700I BbvI BfaI BmyI
BpmI BsaBI BsiYI BslI BsmAI
BsmI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I DdeI DpnII DraI EarI
EcoRI EcoRII EcoT22I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MamI MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NsiI NspI
PleI PshBI RsaI Sau3AI ScrFI
SfaNI SmlI SspBI SspI StuI
TfiI Tru9I TseI Tsp509I TspRI
XhoII XmnI