##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspI        AvrII       BalI        BbeI        
BbsI        BcgI        BglI        BglII       BlnI        
BpuAI       BsaBI       BseAI       BsiEI       BsiWI       
BsmBI       BspDI       BspEI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraIII      DsaI        EagI        EclXI       
Eco47III    Eco52I      EcoO65I     FseI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
StuI        Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII        1577  CGCG                 CG^CG
AcyI         1180  GGCGTC               G[GA]^CG[CT]C
AhdI         3393  GACATTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        3393  GACATTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII          55  TGCGCA               TGC^GCA
BamHI        3135  GGATCC               G^GATCC
BciVI        3412  GGATAC               GTATCC(6/5)
BclI         1583  TGATCA               T^GATCA
BsaHI        1180  GGCGTC               G[GA]^CG[CT]C
BsaWI          73  ACCGGA               [AT]^CCGG[AT]
BseRI        2956  GAGGAG               GAGGAG(10/8)
BsmI         1247  GCATTC               GAATGC(1/-1)
BspLU11I     2742  ACATGT               A^CATGT
BspMI        2704  ACCTGC               ACCTGC(4/8)
BsrFI         527  ACCGGC               [GA]^CCGG[CT]
BssSI        1552  CACGAG               CACGAG(-5/-1)
BstUI        1577  CGCG                 CG^CG
Cfr10I        527  ACCGGC               [GA]^CCGG[CT]
DrdI          587  GACATGGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI           35  TGGCCG               [CT]^GGCC[GA]
Eam1105I     3393  GACATTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1687  GATATC               GAT^ATC
EcoT14I      1032  CCTTGG               C^C[AT][AT]GG
EcoT22I       599  ATGCAT               ATGCA^T
FbaI         1583  TGATCA               T^GATCA
FspI           55  TGCGCA               TGC^GCA
HaeII          61  GGCGCT               [GA]GCGC^[CT]
Hin1I        1180  GGCGTC               G[GA]^CG[CT]C
HincII       1776  GTTAAC               GT[CT]^[GA]AC
HindII       1776  GTTAAC               GT[CT]^[GA]AC
HindIII      2614  AAGCTT               A^AGCTT
HpaI         1776  GTTAAC               GTT^AAC
MvnI         1577  CGCG                 CG^CG
NsiI          599  ATGCAT               ATGCA^T
SbfI         1193  CCTGCAGG             CCTGCA^GG
ScaI          169  AGTACT               AGT^ACT
SexAI          29  ACCTGGT              A^CC[AT]GGT
SpeI         2527  ACTAGT               A^CTAGT
Sse8387I     1193  CCTGCAGG             CCTGCA^GG
StyI         1032  CCTTGG               C^C[AT][AT]GG
SwaI         2217  ATTTAAAT             ATTT^AAAT


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AflII        2849  CTTAAG               C^TTAAG
             2891  CTTAAG              
AflIII       1078  ACACGT               A^C[GA][CT]GT
             2742  ACATGT              
AseI          852  ATTAAT               AT^TAAT
             3533  ATTAAT              
AsnI          852  ATTAAT               AT^TAAT
             3533  ATTAAT              
Asp700I       412  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2924  GAATCCTTTC          
AspHI         673  GAGCAC               G[AT]GC[AT]^C
             1129  GAGCAC              
BanI          294  GGTGCC               G^G[CT][GA]CC
             1448  GGCACC              
BanII        1507  GGGCTC               G[GA]GC[CT]^C
             1546  GGGCTC              
BbrPI        1000  CACGTG               CAC^GTG
             1079  CACGTG              
BfrI         2849  CTTAAG               C^TTAAG
             2891  CTTAAG              
BsaAI        1000  CACGTG               [CT]AC^GT[GA]
             1079  CACGTG              
BsaI         1491  GAGACC               GGTCTC(1/5)
             3629  GAGACC              
BsiHKAI       673  GAGCAC               G[AT]GC[AT]^C
             1129  GAGCAC              
Bsp1407I     1676  TGTACA               T^GTACA
             3440  TGTACA              
BspHI        2083  TCATGA               T^CATGA
             3162  TCATGA              
BsrBI          81  GAGCGG               CCGCTC(-3/-3)
             1630  GAGCGG              
BsrGI        1676  TGTACA               T^GTACA
             3440  TGTACA              
BstAPI        801  GCAGGAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1515  GCAGAGCCTGC         
EcoNI          13  CCTGGGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1452  CCTGGCTGAGG         
PmaCI        1000  CACGTG               CAC^GTG
             1079  CACGTG              
PmlI         1000  CACGTG               CAC^GTG
             1079  CACGTG              
PpuMI          26  AGGACCT              [GA]G^G[AT]CC[CT]
             2256  AGGACCC             
PshBI         852  ATTAAT               AT^TAAT
             3533  ATTAAT              
RcaI         2083  TCATGA               T^CATGA
             3162  TCATGA              
SapI          627  GCTCTTC              GCTCTTC(1/4)
             1788  GCTCTTC             
SmaI          290  CCCGGG               CCC^GGG
             3752  CCCGGG              
SphI         2393  GCATGC               GCATG^C
             3699  GCATGC              
SspBI        1676  TGTACA               T^GTACA
             3440  TGTACA              
XcmI          509  CCAATTCCCCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1041  CCAAGAGGACAATGG     
XmaCI         290  CCCGGG               C^CCGGG
             3752  CCCGGG              
XmaI          290  CCCGGG               C^CCGGG
             3752  CCCGGG              
XmnI          412  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2924  GAATCCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AvaI        
AvaII       BbvCI       BbvI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BsaJI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       EarI        Eco81I      
EcoO109I    EcoRI       EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII