##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII BamHI BbsI
BciVI BclI BglII BpuAI BsaBI
BsiWI BsmI BspDI BspHI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MamI MfeI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 840 GACGTC GACGT^C
AccI 97 GTCGAC GT^[AC][GT]AC
AccIII 160 TCCGGA T^CCGGA
AcsI 4803 AAATTT [GA]^AATT[CT]
AflII 4510 CTTAAG C^TTAAG
Aor51HI 1307 AGCGCT AGC^GCT
ApoI 4803 AAATTT [GA]^AATT[CT]
Asp700I 4464 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3647 CCTAGG C^CTAGG
BcgI 3183 CGATGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4510 CTTAAG C^TTAAG
BlnI 3647 CCTAGG C^CTAGG
BlpI 1158 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1158 GCTGAGC GC^T[ACGT]AGC
BseAI 160 TCCGGA T^CCGGA
Bsp1407I 1519 TGTACA T^GTACA
BspEI 160 TCCGGA T^CCGGA
BspLU11I 4206 ACATGT A^CATGT
BsrDI 4261 CATTGC GCAATG(2/0)
BsrGI 1519 TGTACA T^GTACA
BstEII 2239 GGTCACC G^GT[ACGT]ACC
BstPI 2239 GGTCACC G^GT[ACGT]ACC
CelII 1158 GCTGAGC GC^T[ACGT]AGC
Eco47III 1307 AGCGCT AGC^GCT
EcoO65I 2239 GGTCACC G^GT[ACGT]ACC
HincII 97 GTCGAC GT[CT]^[GA]AC
HindII 97 GTCGAC GT[CT]^[GA]AC
MluI 1870 ACGCGT A^CGCGT
MroI 160 TCCGGA T^CCGGA
PaeR7I 3174 CTCGAG C^TCGAG
PshAI 2992 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3498 GAGCTC GAGCT^C
SalI 97 GTCGAC G^TCGAC
SspBI 1519 TGTACA T^GTACA
TfiI 493 GATTC G^A[AT]TC
XhoI 3174 CTCGAG C^TCGAG
XmnI 4464 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 2234 CCTGAGG CC^T[ACGT]AGG
2676 CCTGAGG
BbeI 203 GGCGCC GGCGC^C
2181 GGCGCC
BsmBI 708 GAGACG CGTCTC(1/5)
1922 GAGACG
BssSI 1715 CACGAG CACGAG(-5/-1)
3800 CTCGTG
BstYI 788 GGATCT [GA]^GATC[CT]
4425 GGATCT
Bsu36I 2234 CCTGAGG CC^T[ACGT]AGG
2676 CCTGAGG
DraI 4795 TTTAAA TTT^AAA
4807 TTTAAA
Eco81I 2234 CCTGAGG CC^T[ACGT]AGG
2676 CCTGAGG
KasI 203 GGCGCC G^GCGCC
2181 GGCGCC
MflI 788 GGATCT [GA]^GATC[CT]
4425 GGATCT
NarI 203 GGCGCC GG^CGCC
2181 GGCGCC
NspI 1507 ACATGC [GA]CATG^[CT]
4206 ACATGT
SapI 365 GCTCTTC GCTCTTC(1/4)
3300 GCTCTTC
ScaI 1033 AGTACT AGT^ACT
1063 AGTACT
SfoI 203 GGCGCC GGC^GCC
2181 GGCGCC
SmaI 122 CCCGGG CCC^GGG
2448 CCCGGG
SmlI 3174 CTCGAG C^T[CT][GA]AG
4510 CTTAAG
XhoII 788 GGATCT [GA]^GATC[CT]
4425 GGATCT
XmaCI 122 CCCGGG C^CCGGG
2448 CCCGGG
XmaI 122 CCCGGG C^CCGGG
2448 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI