##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI Alw44I AlwNI ApaI ApaLI
AscI AseI AsnI Asp718I AviII
BbeI BbvCI BsaHI BsiEI BsiWI
BsmBI BsmI BspDI BspHI BspLU11I
BssHII BstBI BstEII BstPI BstUI
ClaI CpoI DraIII EagI EclXI
Eco52I EcoO65I EcoRV FseI FspI
Hin1I HpaI KasI KpnI KspI
MfeI MluI MunI MvnI NarI
NcoI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 213 TCCGGA T^CCGGA
AflII 1499 CTTAAG C^TTAAG
AflIII 3707 ACACGT A^C[GA][CT]GT
AhdI 2068 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2068 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3824 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 66 CCTAGG C^CTAGG
BalI 2535 TGGCCA TGG^CCA
BamHI 1029 GGATCC G^GATCC
BanI 2743 GGTGCC G^G[CT][GA]CC
BbrPI 758 CACGTG CAC^GTG
BbsI 3187 GTCTTC GAAGAC(2/6)
BcgI 3308 CGAACAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1499 CTTAAG C^TTAAG
BglII 1169 AGATCT A^GATCT
BlnI 66 CCTAGG C^CTAGG
BpuAI 3187 GTCTTC GAAGAC(2/6)
BsaBI 4140 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 213 TCCGGA [AT]^CCGG[AT]
BseAI 213 TCCGGA T^CCGGA
Bsp1407I 892 TGTACA T^GTACA
BspEI 213 TCCGGA T^CCGGA
BsrFI 3154 GCCGGC [GA]^CCGG[CT]
BsrGI 892 TGTACA T^GTACA
BssSI 2303 CACGAG CACGAG(-5/-1)
Bst1107I 4595 GTATAC GTA^TAC
BstZ17I 4595 GTATAC GTA^TAC
Cfr10I 3154 GCCGGC [GA]^CCGG[CT]
DrdI 2181 GACACAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 224 CCACGG C^C[GA][CT]GG
Eam1105I 2068 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2750 GAATTC G^AATTC
EcoT22I 4737 ATGCAT ATGCA^T
MamI 4140 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2535 TGGCCA TGG^CCA
MroI 213 TCCGGA T^CCGGA
MscI 2535 TGGCCA TGG^CCA
NaeI 3154 GCCGGC GCC^GGC
NdeI 1623 CATATG CA^TATG
NgoMIV 3154 GCCGGC G^CCGGC
NheI 2200 GCTAGC G^CTAGC
NsiI 4737 ATGCAT ATGCA^T
PaeR7I 1996 CTCGAG C^TCGAG
PflFI 3824 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 758 CACGTG CAC^GTG
PmlI 758 CACGTG CAC^GTG
SacI 3740 GAGCTC GAGCT^C
SphI 3211 GCATGC GCATG^C
SspBI 892 TGTACA T^GTACA
Tth111I 3824 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1996 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3752 GTCTAC GT^[AC][GT]AC
4595 GTATAC
Aor51HI 2019 AGCGCT AGC^GCT
4263 AGCGCT
BciVI 2421 GGATAC GTATCC(6/5)
1813 GTATCC
BclI 1164 TGATCA T^GATCA
3868 TGATCA
BglI 1268 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2537 GCCAAGTGGGC
BlpI 1942 GCTAAGC GC^T[ACGT]AGC
4286 GCTGAGC
Bpu1102I 1942 GCTAAGC GC^T[ACGT]AGC
4286 GCTGAGC
BsaAI 758 CACGTG [CT]AC^GT[GA]
3229 CACGTA
BsaI 1176 GAGACC GGTCTC(1/5)
1993 GGTCTC
BsrBI 627 GAGCGG CCGCTC(-3/-3)
1986 GAGCGG
BstAPI 78 GCATCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
326 GCAGAAAGTGC
BstXI 123 CCAAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
900 CCAGGTTCCTGG
CelII 1942 GCTAAGC GC^T[ACGT]AGC
4286 GCTGAGC
CfoI 2020 GCGC GCG^C
4264 GCGC
DraI 3724 TTTAAA TTT^AAA
4727 TTTAAA
Eco47III 2019 AGCGCT AGC^GCT
4263 AGCGCT
FauI 192 CCCGC CCCGC(4/6)
629 GCGGG
FbaI 1164 TGATCA T^GATCA
3868 TGATCA
HaeII 2019 AGCGCT [GA]GCGC^[CT]
4263 AGCGCT
HhaI 2020 GCGC GCG^C
4264 GCGC
HinP1I 2020 GCGC G^CGC
4264 GCGC
HincII 455 GTTGAC GT[CT]^[GA]AC
2135 GTCAAC
HindII 455 GTTGAC GT[CT]^[GA]AC
2135 GTCAAC
HindIII 878 AAGCTT A^AGCTT
1347 AAGCTT
PflMI 4182 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4217 CCATATCCTGG
Van91I 4182 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4217 CCATATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI Asp700I AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI