##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BcgI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaI
BsiEI BsiWI BssHII BssSI BstBI
BstEII BstPI BstXI CelII CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FbaI FseI
FspI KasI KpnI KspI MamI
MluI NaeI NarI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 3057 GTATAC GT^[AC][GT]AC
AccIII 2203 TCCGGA T^CCGGA
AcyI 1245 GGCGTC G[GA]^CG[CT]C
AflIII 3200 ACATGT A^C[GA][CT]GT
AlwNI 1491 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3394 CCTCAGG CC^T[ACGT]AGG
BalI 2458 TGGCCA TGG^CCA
BamHI 3479 GGATCC G^GATCC
BglII 1819 AGATCT A^GATCT
BpmI 2373 CTCCAG CTGGAG(16/14)
BsaAI 3623 CACGTA [CT]AC^GT[GA]
BsaHI 1245 GGCGTC G[GA]^CG[CT]C
BsaWI 2203 TCCGGA [AT]^CCGG[AT]
BseAI 2203 TCCGGA T^CCGGA
BsmBI 3892 CGTCTC CGTCTC(1/5)
Bsp1407I 1767 TGTACA T^GTACA
BspDI 1873 ATCGAT AT^CGAT
BspEI 2203 TCCGGA T^CCGGA
BspLU11I 3200 ACATGT A^CATGT
BsrBI 336 CCGCTC CCGCTC(-3/-3)
BsrFI 896 GCCGGT [GA]^CCGG[CT]
BsrGI 1767 TGTACA T^GTACA
Bst1107I 3057 GTATAC GTA^TAC
BstAPI 4307 GCATGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3057 GTATAC GTA^TAC
Bsu36I 3394 CCTCAGG CC^T[ACGT]AGG
Cfr10I 896 GCCGGT [GA]^CCGG[CT]
ClaI 1873 ATCGAT AT^CGAT
DrdI 1953 GACTATTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3394 CCTCAGG CC^T[ACGT]AGG
EcoNI 3742 CCTAGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 698 GAATTC G^AATTC
EcoRV 4138 GATATC GAT^ATC
HaeII 3507 AGCGCC [GA]GCGC^[CT]
Hin1I 1245 GGCGTC G[GA]^CG[CT]C
HindIII 3914 AAGCTT A^AGCTT
HpaI 3313 GTTAAC GTT^AAC
MfeI 3411 CAATTG C^AATTG
MluNI 2458 TGGCCA TGG^CCA
MroI 2203 TCCGGA T^CCGGA
MscI 2458 TGGCCA TGG^CCA
MunI 3411 CAATTG C^AATTG
NheI 2144 GCTAGC G^CTAGC
SapI 755 GCTCTTC GCTCTTC(1/4)
SmaI 172 CCCGGG CCC^GGG
SspBI 1767 TGTACA T^GTACA
TaqI 1874 TCGA T^CGA
TthHB8I 1874 TCGA T^CGA
XcmI 2859 CCAAAATTTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 172 CCCGGG C^CCGGG
XmaI 172 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AseI 3087 ATTAAT AT^TAAT
4501 ATTAAT
AsnI 3087 ATTAAT AT^TAAT
4501 ATTAAT
AspHI 875 GAGCAC G[AT]GC[AT]^C
4103 GTGCTC
AvaI 140 CTCGGG C^[CT]CG[GA]G
172 CCCGGG
BciVI 3694 GGATAC GTATCC(6/5)
1622 GTATCC
BmyI 875 GAGCAC G[AGT]GC[ACT]^C
4103 GTGCTC
BsgI 1707 GTGCAG GTGCAG(16/14)
2678 GTGCAG
BsiHKAI 875 GAGCAC G[AT]GC[AT]^C
4103 GTGCTC
BsoBI 140 CTCGGG C^[CT]CG[GA]G
172 CCCGGG
Bsp1286I 875 GAGCAC G[AGT]GC[ACT]^C
4103 GTGCTC
BspHI 3366 TCATGA T^CATGA
3436 TCATGA
CfoI 133 GCGC GCG^C
3508 GCGC
DraII 154 AGGTCCC [GA]G^G[ACGT]CC[CT]
168 AGGTCCC
DsaI 1626 CCATGG C^C[GA][CT]GG
3475 CCATGG
EaeI 128 TGGCCG [CT]^GGCC[GA]
2458 TGGCCA
EcoO109I 154 AGGTCCC [GA]G^G[ACGT]CC[CT]
168 AGGTCCC
HgaI 1246 GCGTC GACGC(5/10)
3891 GCGTC
HhaI 133 GCGC GCG^C
3508 GCGC
HinP1I 133 GCGC G^CGC
3508 GCGC
MspA1I 353 CAGCTG C[AC]G^C[GT]G
3336 CAGCTG
NcoI 1626 CCATGG C^CATGG
3475 CCATGG
NdeI 849 CATATG CA^TATG
4165 CATATG
PpuMI 154 AGGTCCC [GA]G^G[AT]CC[CT]
168 AGGTCCC
PshBI 3087 ATTAAT AT^TAAT
4501 ATTAAT
PvuII 353 CAGCTG CAG^CTG
3336 CAGCTG
RcaI 3366 TCATGA T^CATGA
3436 TCATGA
ScaI 1594 AGTACT AGT^ACT
3234 AGTACT
SmlI 1308 CTCAAG C^T[CT][GA]AG
2568 CTCAAG
SpeI 1012 ACTAGT A^CTAGT
2932 ACTAGT
Tsp45I 2963 GTCAC ^GT[GC]AC
3211 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaII BbsI
BbvI BcnI BfaI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII XmnI