##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
ApaLI AscI AseI AsnI AviII
BbeI BbrPI BcgI BfrI BglI
BglII BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco52I EcoRI FseI FspI HgaI
Hin1I KasI KspI MaeII MfeI
MluI MroI MunI MvnI NarI
NdeI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AhdI 1801 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3233 AGCGCT AGC^GCT
Asp700I 3450 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1801 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2841 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2571 GGATCC G^GATCC
BclI 2744 TGATCA T^GATCA
BspMI 307 ACCTGC ACCTGC(4/8)
BsrBI 1272 GAGCGG CCGCTC(-3/-3)
Eam1105I 1801 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3233 AGCGCT AGC^GCT
EcoT22I 3384 ATGCAT ATGCA^T
FbaI 2744 TGATCA T^GATCA
HaeII 3233 AGCGCT [GA]GCGC^[CT]
HindIII 4094 AAGCTT A^AGCTT
NsiI 3384 ATGCAT ATGCA^T
PflFI 2841 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 950 GAAGAGC GCTCTTC(1/4)
SexAI 2492 ACCAGGT A^CC[AT]GGT
SmaI 1405 CCCGGG CCC^GGG
StuI 3598 AGGCCT AGG^CCT
Tth111I 2841 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1405 CCCGGG C^CCGGG
XmaI 1405 CCCGGG C^CCGGG
XmnI 3450 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 2500 GGTACC G^GTACC
3163 GGTACC
AcsI 929 AAATTT [GA]^AATT[CT]
3554 GAATTT
AocI 2635 CCTTAGG CC^T[ACGT]AGG
3901 CCTTAGG
ApaI 1081 GGGCCC GGGCC^C
3189 GGGCCC
ApoI 929 AAATTT [GA]^AATT[CT]
3554 GAATTT
Asp718I 2500 GGTACC G^GTACC
3163 GGTACC
AvrII 1022 CCTAGG C^CTAGG
2504 CCTAGG
BalI 1138 TGGCCA TGG^CCA
2165 TGGCCA
BciVI 3956 GTATCC GTATCC(6/5)
4041 GTATCC
BlnI 1022 CCTAGG C^CTAGG
2504 CCTAGG
BsaBI 182 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4106 GATATCCATC
BsrFI 700 GCCGGC [GA]^CCGG[CT]
2007 GCCGGC
BstYI 1315 AGATCC [GA]^GATC[CT]
2571 GGATCC
Bsu36I 2635 CCTTAGG CC^T[ACGT]AGG
3901 CCTTAGG
CfoI 1144 GCGC GCG^C
3234 GCGC
Cfr10I 700 GCCGGC [GA]^CCGG[CT]
2007 GCCGGC
Eco81I 2635 CCTTAGG CC^T[ACGT]AGG
3901 CCTTAGG
EcoRV 25 GATATC GAT^ATC
4106 GATATC
HhaI 1144 GCGC GCG^C
3234 GCGC
HinP1I 1144 GCGC G^CGC
3234 GCGC
HpaI 1209 GTTAAC GTT^AAC
2308 GTTAAC
KpnI 2500 GGTACC GGTAC^C
3163 GGTACC
MamI 182 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4106 GATATCCATC
MflI 1315 AGATCC [GA]^GATC[CT]
2571 GGATCC
MluNI 1138 TGGCCA TGG^CCA
2165 TGGCCA
MscI 1138 TGGCCA TGG^CCA
2165 TGGCCA
NaeI 700 GCCGGC GCC^GGC
2007 GCCGGC
NgoMIV 700 GCCGGC G^CCGGC
2007 GCCGGC
NspI 3342 ACATGC [GA]CATG^[CT]
3382 ACATGC
PaeR7I 591 CTCGAG C^TCGAG
1973 CTCGAG
XhoI 591 CTCGAG C^TCGAG
1973 CTCGAG
XhoII 1315 AGATCC [GA]^GATC[CT]
2571 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI Cac8I CelII
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI