##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccII AciI AclI
AcyI AgeI AhdI Alw44I ApaI
ApaLI AscI Asp700I Asp718I AspEI
AviII AvrII BbeI BclI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsiEI BsiWI BsmBI BspDI BspHI
BspMI BsrBI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI CelII
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I FauI FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NarI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 1144 TCCGGA T^CCGGA
AflII 355 CTTAAG C^TTAAG
AocI 2134 CCTAAGG CC^T[ACGT]AGG
Aor51HI 95 AGCGCT AGC^GCT
AspI 3826 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 969 CTCGGG C^[CT]CG[GA]G
BamHI 831 GGATCC G^GATCC
BanI 66 GGTGCC G^G[CT][GA]CC
BanII 74 GGGCTC G[GA]GC[CT]^C
BbrPI 1444 CACGTG CAC^GTG
BbvCI 3352 GCTGAGG CCTCAGC(-5/-2)
BcgI 1826 CGAAAAGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 866 GGATAC GTATCC(6/5)
BcnI 207 CCGGG CC^[GC]GG
BfrI 355 CTTAAG C^TTAAG
BsaAI 1444 CACGTG [CT]AC^GT[GA]
BsaI 847 GAGACC GGTCTC(1/5)
BsaWI 1144 TCCGGA [AT]^CCGG[AT]
BseAI 1144 TCCGGA T^CCGGA
BsmI 543 GAATGC GAATGC(1/-1)
BsoBI 969 CTCGGG C^[CT]CG[GA]G
Bsp1407I 3037 TGTACA T^GTACA
BspEI 1144 TCCGGA T^CCGGA
BsrFI 107 GCCGGC [GA]^CCGG[CT]
BsrGI 3037 TGTACA T^GTACA
Bst1107I 3732 GTATAC GTA^TAC
BstZ17I 3732 GTATAC GTA^TAC
Bsu36I 2134 CCTAAGG CC^T[ACGT]AGG
Cfr10I 107 GCCGGC [GA]^CCGG[CT]
DraIII 2741 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 95 AGCGCT AGC^GCT
Eco81I 2134 CCTAAGG CC^T[ACGT]AGG
EcoNI 51 CCTGTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 453 GAATTC G^AATTC
EcoT22I 2963 ATGCAT ATGCA^T
HindIII 1541 AAGCTT A^AGCTT
MaeII 1445 ACGT A^CGT
MroI 1144 TCCGGA T^CCGGA
NaeI 107 GCCGGC GCC^GGC
NciI 207 CCGGG CC^[GC]GG
NdeI 2557 CATATG CA^TATG
NgoMIV 107 GCCGGC G^CCGGC
NsiI 2963 ATGCAT ATGCA^T
PflFI 3826 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1444 CACGTG CAC^GTG
PmlI 1444 CACGTG CAC^GTG
PpuMI 1330 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 2670 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 2902 AGTACT AGT^ACT
SspBI 3037 TGTACA T^GTACA
Tth111I 3826 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2381 CCAGAGTCGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AseI 1936 ATTAAT AT^TAAT
3686 ATTAAT
AsnI 1936 ATTAAT AT^TAAT
3686 ATTAAT
AspHI 1393 GAGCAC G[AT]GC[AT]^C
1447 GTGCTC
BalI 2393 TGGCCA TGG^CCA
3224 TGGCCA
BpmI 1921 CTCCAG CTGGAG(16/14)
538 CTGGAG
BseRI 45 CTCCTC GAGGAG(10/8)
329 CTCCTC
BsiHKAI 1393 GAGCAC G[AT]GC[AT]^C
1447 GTGCTC
BsmFI 1332 GTCCC GGGAC(10/14)
2422 GTCCC
BsrDI 458 CATTGC GCAATG(2/0)
2474 CATTGC
DraII 1280 AGGGCCT [GA]G^G[ACGT]CC[CT]
1330 GGGTCCC
EaeI 2393 TGGCCA [CT]^GGCC[GA]
3224 TGGCCA
EcoO109I 1280 AGGGCCT [GA]G^G[ACGT]CC[CT]
1330 GGGTCCC
EcoRV 940 GATATC GAT^ATC
2932 GATATC
HaeII 95 AGCGCT [GA]GCGC^[CT]
110 GGCGCT
MluNI 2393 TGGCCA TGG^CCA
3224 TGGCCA
MscI 2393 TGGCCA TGG^CCA
3224 TGGCCA
MspA1I 440 CAGCTG C[AC]G^C[GT]G
3350 CAGCTG
NcoI 731 CCATGG C^CATGG
2186 CCATGG
PflMI 57 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
885 CCAAGAATTGG
PshBI 1936 ATTAAT AT^TAAT
3686 ATTAAT
PvuII 440 CAGCTG CAG^CTG
3350 CAGCTG
SfcI 2653 CTATAG C^T[GA][CT]AG
3192 CTGTAG
Van91I 57 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
885 CCAAGAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AvaII BbsI
BbvI BfaI BglII BmyI BpuAI
BsaJI BsgI BsiYI BslI BsmAI
Bsp1286I BspLU11I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII