##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BciVI BclI BlnI BsaBI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrGI BssHII Bst1107I BstEII BstPI
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoO65I EcoRV FbaI FseI HgaI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NdeI NotI NruI PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccII 5650 CGCG CG^CG
AcyI 3333 GGCGCC G[GA]^CG[CT]C
AflII 730 CTTAAG C^TTAAG
AviII 2243 TGCGCA TGC^GCA
BbeI 3333 GGCGCC GGCGC^C
BbrPI 5530 CACGTG CAC^GTG
BfrI 730 CTTAAG C^TTAAG
BsaHI 3333 GGCGCC G[GA]^CG[CT]C
BspHI 2900 TCATGA T^CATGA
BstUI 5650 CGCG CG^CG
DraI 5474 TTTAAA TTT^AAA
DrdI 1544 GACTCGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4995 GAATTC G^AATTC
EcoT22I 5021 ATGCAT ATGCA^T
FspI 2243 TGCGCA TGC^GCA
Hin1I 3333 GGCGCC G[GA]^CG[CT]C
KasI 3333 GGCGCC G^GCGCC
MvnI 5650 CGCG CG^CG
NaeI 1441 GCCGGC GCC^GGC
NarI 3333 GGCGCC GG^CGCC
NgoMIV 1441 GCCGGC G^CCGGC
NheI 1567 GCTAGC G^CTAGC
NsiI 5021 ATGCAT ATGCA^T
PmaCI 5530 CACGTG CAC^GTG
PmlI 5530 CACGTG CAC^GTG
PshAI 3927 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2900 TCATGA T^CATGA
SfoI 3333 GGCGCC GGC^GCC
SmaI 2393 CCCGGG CCC^GGG
SspI 274 AATATT AAT^ATT
StuI 3681 AGGCCT AGG^CCT
XmaCI 2393 CCCGGG C^CCGGG
XmaI 2393 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 4995 GAATTC [GA]^AATT[CT]
5084 AAATTT
ApoI 4995 GAATTC [GA]^AATT[CT]
5084 AAATTT
Asp700I 1122 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5311 GAAGTGCTTC
AspI 3808 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3844 GACAAGGTC
BbsI 76 GAAGAC GAAGAC(2/6)
3924 GAAGAC
BbvCI 4861 CCTCAGC CCTCAGC(-5/-2)
103 GCTGAGG
BcgI 477 GCATCAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2315 GCAAGATGCTCG
BglII 2079 AGATCT A^GATCT
2748 AGATCT
BpuAI 76 GAAGAC GAAGAC(2/6)
3924 GAAGAC
BsaAI 5530 CACGTG [CT]AC^GT[GA]
5640 TACGTG
BsaWI 1648 ACCGGA [AT]^CCGG[AT]
3562 TCCGGT
BsiEI 1443 CGGCCG CG[GA][CT]^CG
5497 CGGCCG
BsmI 1596 GAATGC GAATGC(1/-1)
3006 GAATGC
BspLU11I 1164 ACATGT A^CATGT
4937 ACATGT
BssSI 1156 CACGAG CACGAG(-5/-1)
3011 CTCGTG
BstBI 1220 TTCGAA TT^CGAA
2414 TTCGAA
BstXI 3716 CCACCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3729 CCAGACTGCTGG
DraIII 243 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2672 CACCCAGTG
EagI 1443 CGGCCG C^GGCCG
5497 CGGCCG
EclXI 1443 CGGCCG C^GGCCG
5497 CGGCCG
Eco52I 1443 CGGCCG C^GGCCG
5497 CGGCCG
HindIII 2708 AAGCTT A^AGCTT
2918 AAGCTT
NcoI 98 CCATGG C^CATGG
905 CCATGG
NspV 1220 TTCGAA TT^CGAA
2414 TTCGAA
PflFI 3808 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3844 GACAAGGTC
PflMI 4410 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4734 CCACCCCCTGG
SapI 541 GAAGAGC GCTCTTC(1/4)
1360 GAAGAGC
SfiI 1104 GGCCTGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3682 GGCCTCCTCGGCC
SfuI 1220 TTCGAA TT^CGAA
2414 TTCGAA
Tsp45I 1976 GTGAC ^GT[GC]AC
2021 GTCAC
Tth111I 3808 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3844 GACAAGGTC
Van91I 4410 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4734 CCACCCCCTGG
XbaI 993 TCTAGA T^CTAGA
5193 TCTAGA
XmnI 1122 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5311 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XcmI XhoII