##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BbrPI
BcgI BclI BfrI BglII BlnI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrDI BssSI
Bst1107I BstAPI BstZ17I ClaI CpoI
DrdI Eam1105I EcoT22I FbaI FseI
HindIII HpaI KpnI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 2268 GAATTC [GA]^AATT[CT]
Aor51HI 1175 AGCGCT AGC^GCT
ApoI 2268 GAATTC [GA]^AATT[CT]
BalI 1237 TGGCCA TGG^CCA
BamHI 2135 GGATCC G^GATCC
BbeI 1 GGCGCC GGCGC^C
BciVI 2992 GTATCC GTATCC(6/5)
BsaAI 1802 CACGTA [CT]AC^GT[GA]
BsaWI 1807 ACCGGA [AT]^CCGG[AT]
Bsp1407I 89 TGTACA T^GTACA
BsrGI 89 TGTACA T^GTACA
BstBI 1315 TTCGAA TT^CGAA
Eco47III 1175 AGCGCT AGC^GCT
EcoRI 2268 GAATTC G^AATTC
EcoRV 3100 GATATC GAT^ATC
HincII 1704 GTTGAC GT[CT]^[GA]AC
HindII 1704 GTTGAC GT[CT]^[GA]AC
KasI 1 GGCGCC G^GCGCC
MluNI 1237 TGGCCA TGG^CCA
MscI 1237 TGGCCA TGG^CCA
NarI 1 GGCGCC GG^CGCC
NspV 1315 TTCGAA TT^CGAA
PaeR7I 2196 CTCGAG C^TCGAG
SapI 363 GCTCTTC GCTCTTC(1/4)
SexAI 3234 ACCAGGT A^CC[AT]GGT
SfiI 3011 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1 GGCGCC GGC^GCC
SfuI 1315 TTCGAA TT^CGAA
SgrAI 3 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1077 CCCGGG CCC^GGG
SphI 818 GCATGC GCATG^C
SspBI 89 TGTACA T^GTACA
XbaI 2285 TCTAGA T^CTAGA
XhoI 2196 CTCGAG C^TCGAG
XmaCI 1077 CCCGGG C^CCGGG
XmaI 1077 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 974 ACGTGT A^C[GA][CT]GT
1801 ACACGT
AviII 749 TGCGCA TGC^GCA
3758 TGCGCA
BanI 1 GGCGCC G^G[CT][GA]CC
996 GGTGCC
BbsI 312 GAAGAC GAAGAC(2/6)
3317 GAAGAC
BbvCI 566 CCTCAGC CCTCAGC(-5/-2)
2430 CCTCAGC
BpuAI 312 GAAGAC GAAGAC(2/6)
3317 GAAGAC
BssHII 783 GCGCGC G^CGCGC
785 GCGCGC
BstEII 1821 GGTCACC G^GT[ACGT]ACC
3425 GGTGACC
BstPI 1821 GGTCACC G^GT[ACGT]ACC
3425 GGTGACC
BstXI 1409 CCAAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2030 CCATGAAGGTGG
DraI 2515 TTTAAA TTT^AAA
3174 TTTAAA
DraIII 572 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3687 CACAAAGTG
EcoNI 87 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1137 CCTGAAGGAGG
EcoO65I 1821 GGTCACC G^GT[ACGT]ACC
3425 GGTGACC
FspI 749 TGCGCA TGC^GCA
3758 TGCGCA
KspI 237 CCGCGG CCGC^GG
1182 CCGCGG
NaeI 4 GCCGGC GCC^GGC
674 GCCGGC
NgoMIV 4 GCCGGC G^CCGGC
674 GCCGGC
NspI 818 GCATGC [GA]CATG^[CT]
2129 ACATGC
PvuII 1367 CAGCTG CAG^CTG
2561 CAGCTG
SacII 237 CCGCGG CCGC^GG
1182 CCGCGG
TfiI 3672 GATTC G^A[AT]TC
3721 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII