##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AccI        AccIII      AflII       AgeI        AscI        
AseI        AsnI        AviII       BfrI        BsaWI       
BseAI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DrdI        EagI        EclXI       Eco52I      EcoO65I     
EcoRV       FseI        FspI        HindIII     HpaI        
KspI        MfeI        MluI        MroI        MunI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
PvuI        RsrII       SacII       SalI        SfuI        
SgrAI       SnaBI       SpeI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AatII        2256  GACGTC               GACGT^C
Acc65I       1846  GGTACC               G^GTACC
AclI         5080  AACGTT               AA^CGTT
Aor51HI      1873  AGCGCT               AGC^GCT
Asp718I      1846  GGTACC               G^GTACC
AvrII        2327  CCTAGG               C^CTAGG
BbvCI        1928  GCTGAGG              CCTCAGC(-5/-2)
BcgI          553  GCAGCACCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         2327  CCTAGG               C^CTAGG
BsiEI        2592  CGACCG               CG[GA][CT]^CG
DraI         4953  TTTAAA               TTT^AAA
Eco47III     1873  AGCGCT               AGC^GCT
EcoRI        2978  GAATTC               G^AATTC
EcoT22I      5137  ATGCAT               ATGCA^T
KpnI         1846  GGTACC               GGTAC^C
NdeI         5159  CATATG               CA^TATG
NsiI         5137  ATGCAT               ATGCA^T
Psp1406I     5080  AACGTT               AA^CGTT
SexAI        2986  ACCTGGT              A^CC[AT]GGT
SfiI          173  GGCCAGCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         1990  GCATGC               GCATG^C
SspI          870  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   45 enzymes) #
###################################

AhdI          738  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2220  GACCTGAAGTC         
Alw44I       3977  GTGCAC               G^TGCAC
             4788  GTGCAC              
ApaLI        3977  GTGCAC               G^TGCAC
             4788  GTGCAC              
Asp700I      1958  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4735  GAATGAGTTC          
AspEI         738  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2220  GACCTGAAGTC         
AspI         1467  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1638  GACTGTGTC           
BamHI        4426  GGATCC               G^GATCC
             4698  GGATCC              
BbeI         2322  GGCGCC               GGCGC^C
             4032  GGCGCC              
BbrPI         530  CACGTG               CAC^GTG
             5054  CACGTG              
BciVI        2927  GGATAC               GTATCC(6/5)
             5103  GGATAC              
BglII          31  AGATCT               A^GATCT
             3229  AGATCT              
BlpI         2078  GCTCAGC              GC^T[ACGT]AGC
             4796  GCTTAGC             
Bpu1102I     2078  GCTCAGC              GC^T[ACGT]AGC
             4796  GCTTAGC             
BsaBI        1166  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3545  GATCCTGATC          
Bsp1407I     3520  TGTACA               T^GTACA
             5058  TGTACA              
BspHI        1732  TCATGA               T^CATGA
             2557  TCATGA              
BspLU11I     3418  ACATGT               A^CATGT
             5170  ACATGT              
BsrDI        3896  CATTGC               GCAATG(2/0)
             3187  GCAATG              
BsrGI        3520  TGTACA               T^GTACA
             5058  TGTACA              
CelII        2078  GCTCAGC              GC^T[ACGT]AGC
             4796  GCTTAGC             
DraIII         89  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3998  CACAGAGTG           
Eam1105I      738  GACCTGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2220  GACCTGAAGTC         
EarI         1755  CTCTTC               CTCTTC(1/4)
              966  GAAGAG              
KasI         2322  GGCGCC               G^GCGCC
             4032  GGCGCC              
Ksp632I      1755  CTCTTC               CTCTTC(1/4)
              966  GAAGAG              
MamI         1166  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3545  GATCCTGATC          
NaeI          110  GCCGGC               GCC^GGC
             4078  GCCGGC              
NarI         2322  GGCGCC               GG^CGCC
             4032  GGCGCC              
NgoMIV        110  GCCGGC               G^CCGGC
             4078  GCCGGC              
PflFI        1467  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1638  GACTGTGTC           
PmaCI         530  CACGTG               CAC^GTG
             5054  CACGTG              
PmlI          530  CACGTG               CAC^GTG
             5054  CACGTG              
RcaI         1732  TCATGA               T^CATGA
             2557  TCATGA              
SacI           99  GAGCTC               GAGCT^C
             2067  GAGCTC              
SapI          966  GAAGAGC              GCTCTTC(1/4)
             1754  GCTCTTC             
SbfI          611  CCTGCAGG             CCTGCA^GG
             3782  CCTGCAGG            
ScaI          973  AGTACT               AGT^ACT
             1621  AGTACT              
SfoI         2322  GGCGCC               GGC^GCC
             4032  GGCGCC              
SmaI         4151  CCCGGG               CCC^GGG
             4702  CCCGGG              
Sse8387I      611  CCTGCAGG             CCTGCA^GG
             3782  CCTGCAGG            
SspBI        3520  TGTACA               T^GTACA
             5058  TGTACA              
Tth111I      1467  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1638  GACTGTGTC           
XmaCI        4151  CCCGGG               C^CCGGG
             4702  CCCGGG              
XmaI         4151  CCCGGG               C^CCGGG
             4702  CCCGGG              
XmnI         1958  GAAGAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4735  GAATGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  130 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApaI        ApoI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BclI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII