##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AspEI       AviII       AvrII       BbeI        
BbvCI       BciVI       BcnI        BlnI        BsaBI       
BsiWI       BspDI       BspMI       BssHII      BssSI       
BstBI       Bsu36I      ClaI        CpoI        Eam1105I    
Eco47III    Eco81I      FseI        FspI        HaeII       
HpaI        KasI        KspI        MamI        MluI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NspV        PacI        PaeR7I      PmeI        
PvuI        RsrII       SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SmaI        SnaBI       SphI        Sse8387I    SspI        
SwaI        TaqI        TthHB8I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I       2064  GGTACC               G^GTACC
AccIII       2697  TCCGGA               T^CCGGA
AflII        4031  CTTAAG               C^TTAAG
AgeI         2526  ACCGGT               A^CCGGT
ApaI         2000  GGGCCC               GGGCC^C
AseI         4858  ATTAAT               AT^TAAT
AsnI         4858  ATTAAT               AT^TAAT
Asp700I       961  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2064  GGTACC               G^GTACC
BbrPI        3854  CACGTG               CAC^GTG
BfrI         4031  CTTAAG               C^TTAAG
BglII         115  AGATCT               A^GATCT
BsaAI        3854  CACGTG               [CT]AC^GT[GA]
BseAI        2697  TCCGGA               T^CCGGA
BsmBI        1985  CGTCTC               CGTCTC(1/5)
BspEI        2697  TCCGGA               T^CCGGA
BspLU11I      785  ACATGT               A^CATGT
BsrBI        5126  GAGCGG               CCGCTC(-3/-3)
BsrFI        2526  ACCGGT               [GA]^CCGG[CT]
Bst1107I     3894  GTATAC               GTA^TAC
BstEII       4652  GGTCACC              G^GT[ACGT]ACC
BstPI        4652  GGTCACC              G^GT[ACGT]ACC
BstZ17I      3894  GTATAC               GTA^TAC
CfoI         2886  GCGC                 GCG^C
Cfr10I       2526  ACCGGT               [GA]^CCGG[CT]
DraI         4297  TTTAAA               TTT^AAA
DraIII       2150  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2950  GACAGTCTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         5129  CGGCCG               C^GGCCG
EclXI        5129  CGGCCG               C^GGCCG
Eco52I       5129  CGGCCG               C^GGCCG
EcoO65I      4652  GGTCACC              G^GT[ACGT]ACC
EcoRI        3779  GAATTC               G^AATTC
EcoRV        3735  GATATC               GAT^ATC
EcoT22I      4785  ATGCAT               ATGCA^T
HhaI         2886  GCGC                 GCG^C
HinP1I       2886  GCGC                 G^CGC
KpnI         2064  GGTACC               GGTAC^C
MfeI         4712  CAATTG               C^AATTG
MroI         2697  TCCGGA               T^CCGGA
MunI         4712  CAATTG               C^AATTG
NotI         5128  GCGGCCGC             GC^GGCCGC
NruI         5122  TCGCGA               TCG^CGA
NsiI         4785  ATGCAT               ATGCA^T
PflMI        3698  CCAGAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2526  ACCGGT               A^CCGGT
PmaCI        3854  CACGTG               CAC^GTG
PmlI         3854  CACGTG               CAC^GTG
PshAI        1351  GACTATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4858  ATTAAT               AT^TAAT
SgrAI        2525  CACCGGTG             C[GA]^CCGG[CT]G
StuI          443  AGGCCT               AGG^CCT
Van91I       3698  CCAGAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         5116  TCTAGA               T^CTAGA
XmnI          961  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AatII         421  GACGTC               GACGT^C
             2844  GACGTC              
AccI         3894  GTATAC               GT^[AC][GT]AC
             4707  GTAGAC              
AccII         528  CGCG                 CG^CG
             5123  CGCG                
AclI         1303  AACGTT               AA^CGTT
             3639  AACGTT              
AcsI         3145  GAATTT               [GA]^AATT[CT]
             3779  GAATTC              
AcyI          421  GACGTC               G[GA]^CG[CT]C
             2844  GACGTC              
ApoI         3145  GAATTT               [GA]^AATT[CT]
             3779  GAATTC              
AspI          418  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1515  GACAGAGTC           
BamHI        1454  GGATCC               G^GATCC
             1567  GGATCC              
BcgI          420  CGACGTCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3229  GCAGTTTTCTCG        
BglI          220  GCCTTGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2661  GCCCAAGTGGC         
BsaHI         421  GACGTC               G[GA]^CG[CT]C
             2844  GACGTC              
BsaWI        2526  ACCGGT               [AT]^CCGG[AT]
             2697  TCCGGA              
BsiEI        2537  CGGTCG               CG[GA][CT]^CG
             5129  CGGCCG              
BsmI         1980  GAATGC               GAATGC(1/-1)
             1992  GCATTC              
BspHI        3731  TCATGA               T^CATGA
             4572  TCATGA              
BstAPI       1918  GCACCTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2149  GCACCGTGTGC         
BstUI         528  CGCG                 CG^CG
             5123  CGCG                
BstXI        2330  CCAAGACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3280  CCACAACAATGG        
HapII        2527  CCGG                 C^CGG
             2698  CCGG                
Hin1I         421  GACGTC               G[GA]^CG[CT]C
             2844  GACGTC              
HincII       1300  GTCAAC               GT[CT]^[GA]AC
             3061  GTCAAC              
HindII       1300  GTCAAC               GT[CT]^[GA]AC
             3061  GTCAAC              
HindIII       997  AAGCTT               A^AGCTT
             4553  AAGCTT              
HpaII        2527  CCGG                 C^CGG
             2698  CCGG                
MspI         2527  CCGG                 C^CGG
             2698  CCGG                
MvnI          528  CGCG                 CG^CG
             5123  CGCG                
PflFI         418  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1515  GACAGAGTC           
Psp1406I     1303  AACGTT               AA^CGTT
             3639  AACGTT              
RcaI         3731  TCATGA               T^CATGA
             4572  TCATGA              
SacI          253  GAGCTC               GAGCT^C
             1677  GAGCTC              
SpeI         4336  ACTAGT               A^CTAGT
             5110  ACTAGT              
Tth111I       418  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1515  GACAGAGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AciI        AfaI        AflIII      AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BclI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    Bsp1407I    BsrDI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CelII       Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeIII      HgaI        HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SspBI       
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XcmI        XhoII