##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       AccIII      AcyI        AgeI        AhdI        
Aor51HI     ApaI        AscI        AseI        AsnI        
AspEI       AvaI        AviII       BamHI       BanII       
BbeI        BbrPI       BcgI        BciVI       BclI        
BglI        BsaAI       BsaHI       BsaWI       BseAI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DrdI        Eam1105I    Eco47III    
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HpaI        KasI        KspI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NgoMIV      NheI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshBI       
PstI        PvuI        RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I       2121  GGTACC               G^GTACC
AclI         2187  AACGTT               AA^CGTT
Alw44I       1503  GTGCAC               G^TGCAC
AlwNI        3920  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1503  GTGCAC               G^TGCAC
Asp718I      2121  GGTACC               G^GTACC
AspI         3190  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BlpI          136  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      136  GCTGAGC              GC^T[ACGT]AGC
BsrFI        2968  GCCGGT               [GA]^CCGG[CT]
BstAPI       4834  GCATAGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        793  GGTCACC              G^GT[ACGT]ACC
BstPI         793  GGTCACC              G^GT[ACGT]ACC
CelII         136  GCTGAGC              GC^T[ACGT]AGC
CfoI         5344  GCGC                 GCG^C
Cfr10I       2968  GCCGGT               [GA]^CCGG[CT]
DsaI         4405  CCATGG               C^C[GA][CT]GG
EcoO65I       793  GGTCACC              G^GT[ACGT]ACC
HhaI         5344  GCGC                 GCG^C
HinP1I       5344  GCGC                 G^CGC
HincII       4311  GTCAAC               GT[CT]^[GA]AC
HindII       4311  GTCAAC               GT[CT]^[GA]AC
KpnI         2121  GGTACC               GGTAC^C
NcoI         4405  CCATGG               C^CATGG
NdeI         5445  CATATG               CA^TATG
NotI         5693  GCGGCCGC             GC^GGCCGC
NruI         5687  TCGCGA               TCG^CGA
PflFI        3190  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2758  CCACCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        5399  GACAGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2187  AACGTT               AA^CGTT
SapI          334  GCTCTTC              GCTCTTC(1/4)
ScaI         2556  AGTACT               AGT^ACT
SpeI         2112  ACTAGT               A^CTAGT
SwaI         3634  ATTTAAAT             ATTT^AAAT
Tth111I      3190  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2758  CCACCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AccI         2627  GTAGAC               GT^[AC][GT]AC
             4116  GTCTAC              
AccII        5343  CGCG                 CG^CG
             5688  CGCG                
AflII        2140  CTTAAG               C^TTAAG
             3446  CTTAAG              
AflIII        100  ACATGT               A^C[GA][CT]GT
             4142  ACATGT              
AvaII        1892  GGACC                G^G[AT]CC
             3972  GGTCC               
AvrII        2359  CCTAGG               C^CTAGG
             2608  CCTAGG              
BbvCI        2783  CCTCAGC              CCTCAGC(-5/-2)
             3280  CCTCAGC             
BcnI         2118  CCGGG                CC^[GC]GG
             2964  CCCGG               
BfrI         2140  CTTAAG               C^TTAAG
             3446  CTTAAG              
BglII         318  AGATCT               A^GATCT
             4548  AGATCT              
BlnI         2359  CCTAGG               C^CTAGG
             2608  CCTAGG              
BsaBI         313  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1378  GATACACATC          
BseRI        2753  CTCCTC               GAGGAG(10/8)
                4  GAGGAG              
BsiEI        2966  CGGCCG               CG[GA][CT]^CG
             5694  CGGCCG              
BspHI        1635  TCATGA               T^CATGA
             3501  TCATGA              
BspLU11I      100  ACATGT               A^CATGT
             4142  ACATGT              
BsrBI        2094  CCGCTC               CCGCTC(-3/-3)
             5691  GAGCGG              
BstUI        5343  CGCG                 CG^CG
             5688  CGCG                
EagI         2966  CGGCCG               C^GGCCG
             5694  CGGCCG              
EclXI        2966  CGGCCG               C^GGCCG
             5694  CGGCCG              
Eco52I       2966  CGGCCG               C^GGCCG
             5694  CGGCCG              
EcoNI         771  CCTTACACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5499  CCTGCCTCAGG         
EcoRV        3717  GATATC               GAT^ATC
             3908  GATATC              
EcoT22I       282  ATGCAT               ATGCA^T
             3674  ATGCAT              
FauI         2829  CCCGC                CCCGC(4/6)
             5168  GCGGG               
MaeII        1898  ACGT                 A^CGT
             2188  ACGT                
MamI          313  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1378  GATACACATC          
MvnI         5343  CGCG                 CG^CG
             5688  CGCG                
NciI         2118  CCGGG                CC^[GC]GG
             2964  CCCGG               
NsiI          282  ATGCAT               ATGCA^T
             3674  ATGCAT              
PpuMI        1891  GGGACCT              [GA]G^G[AT]CC[CT]
             3971  GGGTCCC             
RcaI         1635  TCATGA               T^CATGA
             3501  TCATGA              
SphI          284  GCATGC               GCATG^C
             2813  GCATGC              
SspI         4197  AATATT               AAT^ATT
             4867  AATATT              
TaqI         3385  TCGA                 T^CGA
             4340  TCGA                
TthHB8I      3385  TCGA                 T^CGA
             4340  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AocI        ApoI        Asp700I     AspHI       BalI        
BanI        BbsI        BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    Bsp1407I    BspMI       BsrDI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       Cfr13I      
DdeI        DpnII       DraI        DraII       DraIII      
EaeI        EarI        Eco81I      EcoO109I    EcoRI       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
StuI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XbaI        XhoII       
XmnI