##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
AgeI Alw44I Aor51HI ApaLI Asp718I
AspI AviII AvrII BalI BamHI
BbeI BbrPI BlnI BsaAI BsaHI
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrGI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI Hin1I KasI KpnI
KspI MluI MluNI MscI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AscI 4291 GGCGCGCC GG^CGCGCC
BbvCI 2047 GCTGAGG CCTCAGC(-5/-2)
BcgI 94 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1146 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3472 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrFI 4010 ACCGGC [GA]^CCGG[CT]
BssHII 4292 GCGCGC G^CGCGC
BssSI 508 CACGAG CACGAG(-5/-1)
Cfr10I 4010 ACCGGC [GA]^CCGG[CT]
DraIII 1751 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 5490 CGGCCA [CT]^GGCC[GA]
HaeII 306 GGCGCT [GA]GCGC^[CT]
HgaI 513 GCGTC GACGC(5/10)
HincII 3919 GTTAAC GT[CT]^[GA]AC
HindII 3919 GTTAAC GT[CT]^[GA]AC
HpaI 3919 GTTAAC GTT^AAC
MamI 3472 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1559 CAATTG C^AATTG
MunI 1559 CAATTG C^AATTG
NcoI 592 CCATGG C^CATGG
PaeR7I 2507 CTCGAG C^TCGAG
PflMI 5251 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 662 AGGACCC [GA]G^G[AT]CC[CT]
SacI 3025 GAGCTC GAGCT^C
SapI 4572 GAAGAGC GCTCTTC(1/4)
SpeI 627 ACTAGT A^CTAGT
SspI 1079 AATATT AAT^ATT
Van91I 5251 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2507 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 4222 GTCTAC GT^[AC][GT]AC
4532 GTAGAC
AccII 263 CGCG CG^CG
4293 CGCG
AccIII 1977 TCCGGA T^CCGGA
5024 TCCGGA
AflII 3544 CTTAAG C^TTAAG
4614 CTTAAG
AhdI 1225 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1674 GACCCCAGGTC
AseI 1987 ATTAAT AT^TAAT
5185 ATTAAT
AsnI 1987 ATTAAT AT^TAAT
5185 ATTAAT
Asp700I 530 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4275 GAAACCCTTC
AspEI 1225 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1674 GACCCCAGGTC
AvaI 2507 CTCGAG C^[CT]CG[GA]G
4897 CCCGAG
BanI 3124 GGCACC G^G[CT][GA]CC
4105 GGTGCC
BciVI 5154 GGATAC GTATCC(6/5)
1101 GTATCC
BclI 783 TGATCA T^GATCA
4941 TGATCA
BfrI 3544 CTTAAG C^TTAAG
4614 CTTAAG
BglII 448 AGATCT A^GATCT
5339 AGATCT
BseAI 1977 TCCGGA T^CCGGA
5024 TCCGGA
BsmBI 514 CGTCTC CGTCTC(1/5)
5082 CGTCTC
BsoBI 2507 CTCGAG C^[CT]CG[GA]G
4897 CCCGAG
BspEI 1977 TCCGGA T^CCGGA
5024 TCCGGA
BsrBI 539 CCGCTC CCGCTC(-3/-3)
667 CCGCTC
BstEII 1446 GGTTACC G^GT[ACGT]ACC
1723 GGTCACC
BstPI 1446 GGTTACC G^GT[ACGT]ACC
1723 GGTCACC
BstUI 263 CGCG CG^CG
4293 CGCG
Eam1105I 1225 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1674 GACCCCAGGTC
EcoO65I 1446 GGTTACC G^GT[ACGT]ACC
1723 GGTCACC
FbaI 783 TGATCA T^GATCA
4941 TGATCA
MaeII 1778 ACGT A^CGT
4877 ACGT
MroI 1977 TCCGGA T^CCGGA
5024 TCCGGA
MvnI 263 CGCG CG^CG
4293 CGCG
NspI 3676 GCATGT [GA]CATG^[CT]
4751 ACATGC
PshBI 1987 ATTAAT AT^TAAT
5185 ATTAAT
SexAI 2307 ACCAGGT A^CC[AT]GGT
2477 ACCAGGT
XbaI 1188 TCTAGA T^CTAGA
3507 TCTAGA
XcmI 763 CCAGCAGACCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
991 CCAGCTCAGCGTTGG
XmnI 530 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4275 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaII BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV PleI PshAI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII