##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI AocI Aor51HI ApaI
AscI AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BcgI
BclI BcnI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KspI MamI
MluI MroI MvnI NaeI NarI
NciI NcoI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AvaII 2500 GGTCC G^G[AT]CC
BamHI 5273 GGATCC G^GATCC
BsaAI 5129 TACGTG [CT]AC^GT[GA]
BsgI 653 GTGCAG GTGCAG(16/14)
BsmI 988 GAATGC GAATGC(1/-1)
BspHI 5512 TCATGA T^CATGA
BstEII 5450 GGTGACC G^GT[ACGT]ACC
BstPI 5450 GGTGACC G^GT[ACGT]ACC
DraIII 1665 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3623 CCTCTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5450 GGTGACC G^GT[ACGT]ACC
EcoRV 5170 GATATC GAT^ATC
EcoT22I 3980 ATGCAT ATGCA^T
HapII 2205 CCGG C^CGG
HpaII 2205 CCGG C^CGG
MspA1I 2366 CAGCTG C[AC]G^C[GT]G
MspI 2205 CCGG C^CGG
NheI 3916 GCTAGC G^CTAGC
NsiI 3980 ATGCAT ATGCA^T
PpuMI 2499 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 2366 CAGCTG CAG^CTG
RcaI 5512 TCATGA T^CATGA
SbfI 3214 CCTGCAGG CCTGCA^GG
SpeI 5207 ACTAGT A^CTAGT
Sse8387I 3214 CCTGCAGG CCTGCA^GG
StuI 2044 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2890 GGTACC G^GTACC
4460 GGTACC
Alw44I 1597 GTGCAC G^TGCAC
3510 GTGCAC
AlwNI 1604 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2498 CAGGTCCTG
ApaLI 1597 GTGCAC G^TGCAC
3510 GTGCAC
Asp700I 302 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
591 GAATAAGTTC
Asp718I 2890 GGTACC G^GTACC
4460 GGTACC
AspHI 1597 GTGCAC G[AT]GC[AT]^C
3510 GTGCAC
BanI 2890 GGTACC G^G[CT][GA]CC
4460 GGTACC
BanII 1886 GAGCCC G[GA]GC[CT]^C
3416 GGGCTC
BciVI 1121 GGATAC GTATCC(6/5)
3857 GTATCC
BsiHKAI 1597 GTGCAC G[AT]GC[AT]^C
3510 GTGCAC
Bsp1407I 2410 TGTACA T^GTACA
2447 TGTACA
BspLU11I 3720 ACATGT A^CATGT
4343 ACATGT
BsrGI 2410 TGTACA T^GTACA
2447 TGTACA
DraII 1605 AGGCCCT [GA]G^G[ACGT]CC[CT]
2499 AGGTCCT
EcoO109I 1605 AGGCCCT [GA]G^G[ACGT]CC[CT]
2499 AGGTCCT
EcoRI 907 GAATTC G^AATTC
1523 GAATTC
HincII 4576 GTCAAC GT[CT]^[GA]AC
4607 GTCAAC
HindII 4576 GTCAAC GT[CT]^[GA]AC
4607 GTCAAC
KpnI 2890 GGTACC GGTAC^C
4460 GGTACC
MaeII 4337 ACGT A^CGT
5130 ACGT
MwoI 2376 GCAAAAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
4647 GCTTCGTTTGC
NdeI 3330 CATATG CA^TATG
4360 CATATG
PflMI 629 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5310 CCATAGTTTGG
PshAI 2836 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5453 GACCAATGTC
PstI 1398 CTGCAG CTGCA^G
3215 CTGCAG
ScaI 2552 AGTACT AGT^ACT
3193 AGTACT
SphI 2200 GCATGC GCATG^C
4274 GCATGC
SspBI 2410 TGTACA T^GTACA
2447 TGTACA
Van91I 629 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5310 CCATAGTTTGG
XmnI 302 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
591 GAATAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI ApoI AseI
AsnI BalI BbsI BbvI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MunI MvaI NdeII NlaIII
NlaIV NspI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII