##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccII AclI AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp700I AspEI AvaI AviII
BamHI BbeI BbrPI BbvCI BcgI
BcnI BglI BglII BlpI Bpu1102I
BsaAI BsaHI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KspI
MluI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI StuI Van91I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1827 GGTACC G^GTACC
AccIII 3813 TCCGGA T^CCGGA
AflIII 1566 ACATGT A^C[GA][CT]GT
Asp718I 1827 GGTACC G^GTACC
AspHI 1613 GTGCTC G[AT]GC[AT]^C
AspI 3341 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3153 TGGCCA TGG^CCA
BpmI 2154 CTCCAG CTGGAG(16/14)
BsaBI 2202 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3397 GGTCTC GGTCTC(1/5)
BseAI 3813 TCCGGA T^CCGGA
BsiHKAI 1613 GTGCTC G[AT]GC[AT]^C
BsmI 3277 GCATTC GAATGC(1/-1)
BspEI 3813 TCCGGA T^CCGGA
BspHI 4399 TCATGA T^CATGA
BspLU11I 1566 ACATGT A^CATGT
EaeI 3153 TGGCCA [CT]^GGCC[GA]
EcoNI 1905 CCTAAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2391 ATGCAT ATGCA^T
FauI 2476 CCCGC CCCGC(4/6)
HincII 4437 GTCAAC GT[CT]^[GA]AC
HindII 4437 GTCAAC GT[CT]^[GA]AC
KpnI 1827 GGTACC GGTAC^C
MamI 2202 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 499 CAATTG C^AATTG
MluNI 3153 TGGCCA TGG^CCA
MroI 3813 TCCGGA T^CCGGA
MscI 3153 TGGCCA TGG^CCA
MspA1I 470 CAGCTG C[AC]G^C[GT]G
MunI 499 CAATTG C^AATTG
NsiI 2391 ATGCAT ATGCA^T
PflFI 3341 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 470 CAGCTG CAG^CTG
RcaI 4399 TCATGA T^CATGA
SapI 1193 GCTCTTC GCTCTTC(1/4)
SpeI 2898 ACTAGT A^CTAGT
SwaI 5044 ATTTAAAT ATTT^AAAT
Tth111I 3341 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1155 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 4760 CTTAAG C^TTAAG
5418 CTTAAG
ApaI 2473 GGGCCC GGGCC^C
3363 GGGCCC
AvrII 429 CCTAGG C^CTAGG
2701 CCTAGG
BbsI 3625 GAAGAC GAAGAC(2/6)
3877 GAAGAC
BclI 1982 TGATCA T^GATCA
2106 TGATCA
BfrI 4760 CTTAAG C^TTAAG
5418 CTTAAG
BlnI 429 CCTAGG C^CTAGG
2701 CCTAGG
BpuAI 3625 GAAGAC GAAGAC(2/6)
3877 GAAGAC
BsaWI 3552 TCCGGT [AT]^CCGG[AT]
3813 TCCGGA
BspMI 2456 ACCTGC ACCTGC(4/8)
4577 GCAGGT
Bst1107I 1281 GTATAC GTA^TAC
1288 GTATAC
BstYI 1170 GGATCT [GA]^GATC[CT]
3943 AGATCC
BstZ17I 1281 GTATAC GTA^TAC
1288 GTATAC
FbaI 1982 TGATCA T^GATCA
2106 TGATCA
HapII 3553 CCGG C^CGG
3814 CCGG
HpaII 3553 CCGG C^CGG
3814 CCGG
MflI 1170 GGATCT [GA]^GATC[CT]
3943 AGATCC
MspI 3553 CCGG C^CGG
3814 CCGG
PstI 473 CTGCAG CTGCA^G
3009 CTGCAG
TaqI 3759 TCGA T^CGA
4768 TCGA
TthHB8I 3759 TCGA T^CGA
4768 TCGA
XhoII 1170 GGATCT [GA]^GATC[CT]
3943 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AvaII BanI BanII BbvI BciVI
BfaI BmyI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NspI PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI