##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
AocI Aor51HI AscI Asp718I AspEI
AviII AvrII BbeI BbrPI BglI
BlnI BsaBI BseAI BsiWI BsmBI
BspEI BsrFI BssHII BssSI BstEII
BstPI Bsu36I Cfr10I CpoI DrdI
Eam1105I Eco47III Eco81I EcoO65I FseI
FspI KasI KpnI KspI MamI
MluI MroI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 150 GACGTC GACGT^C
AcyI 150 GACGTC G[GA]^CG[CT]C
AflII 4585 CTTAAG C^TTAAG
Alw44I 86 GTGCAC G^TGCAC
ApaI 1083 GGGCCC GGGCC^C
ApaLI 86 GTGCAC G^TGCAC
AspI 1377 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2274 GGATCC G^GATCC
BanI 3148 GGCACC G^G[CT][GA]CC
BcgI 3088 GCAAAAATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 859 TGATCA T^GATCA
BcnI 987 CCCGG CC^[GC]GG
BfrI 4585 CTTAAG C^TTAAG
BglII 138 AGATCT A^GATCT
BlpI 3512 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3512 GCTAAGC GC^T[ACGT]AGC
BsaAI 3533 CACGTA [CT]AC^GT[GA]
BsaHI 150 GACGTC G[GA]^CG[CT]C
BsaWI 2370 ACCGGA [AT]^CCGG[AT]
BsiEI 2764 CGGCCG CG[GA][CT]^CG
BspDI 1518 ATCGAT AT^CGAT
BsrBI 3441 GAGCGG CCGCTC(-3/-3)
BstBI 2446 TTCGAA TT^CGAA
CelII 3512 GCTAAGC GC^T[ACGT]AGC
ClaI 1518 ATCGAT AT^CGAT
DraIII 2352 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2764 CGGCCG C^GGCCG
EclXI 2764 CGGCCG C^GGCCG
Eco52I 2764 CGGCCG C^GGCCG
EcoRI 3605 GAATTC G^AATTC
EcoRV 2742 GATATC GAT^ATC
FbaI 859 TGATCA T^GATCA
HaeII 1437 AGCGCC [GA]GCGC^[CT]
Hin1I 150 GACGTC G[GA]^CG[CT]C
HincII 2360 GTTAAC GT[CT]^[GA]AC
HindII 2360 GTTAAC GT[CT]^[GA]AC
HpaI 2360 GTTAAC GTT^AAC
NciI 987 CCCGG CC^[GC]GG
NspV 2446 TTCGAA TT^CGAA
PflFI 1377 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1571 GACGTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 3001 ACCTGGT A^CC[AT]GGT
SfuI 2446 TTCGAA TT^CGAA
SphI 4161 GCATGC GCATG^C
SwaI 3713 ATTTAAAT ATTT^AAAT
Tth111I 1377 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 27 CGCG CG^CG
2814 CGCG
AflIII 434 ACATGT A^C[GA][CT]GT
5204 ACATGT
AseI 2723 ATTAAT AT^TAAT
4762 ATTAAT
AsnI 2723 ATTAAT AT^TAAT
4762 ATTAAT
AvaI 2986 CTCGGG C^[CT]CG[GA]G
4039 CCCGAG
BbvCI 1895 CCTCAGC CCTCAGC(-5/-2)
2026 CCTCAGC
BseRI 3030 GAGGAG GAGGAG(10/8)
3033 GAGGAG
BsgI 3220 CTGCAC GTGCAG(16/14)
1873 GTGCAG
BsmI 2588 GAATGC GAATGC(1/-1)
1112 GCATTC
BsoBI 2986 CTCGGG C^[CT]CG[GA]G
4039 CCCGAG
BspLU11I 434 ACATGT A^CATGT
5204 ACATGT
BsrDI 389 CATTGC GCAATG(2/0)
4787 CATTGC
Bst1107I 3006 GTATAC GTA^TAC
5502 GTATAC
BstAPI 1013 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4152 GCAACAATTGC
BstUI 27 CGCG CG^CG
2814 CGCG
BstXI 4872 CCACACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5298 CCAGGGAGGTGG
BstZ17I 3006 GTATAC GTA^TAC
5502 GTATAC
DraII 4 AGGTCCT [GA]G^G[ACGT]CC[CT]
601 AGGACCC
EcoO109I 4 AGGTCCT [GA]G^G[ACGT]CC[CT]
601 AGGACCC
EcoT22I 73 ATGCAT ATGCA^T
4268 ATGCAT
HapII 988 CCGG C^CGG
2371 CCGG
HindIII 2072 AAGCTT A^AGCTT
4437 AAGCTT
HpaII 988 CCGG C^CGG
2371 CCGG
MfeI 4156 CAATTG C^AATTG
5174 CAATTG
MspI 988 CCGG C^CGG
2371 CCGG
MunI 4156 CAATTG C^AATTG
5174 CAATTG
MvnI 27 CGCG CG^CG
2814 CGCG
NsiI 73 ATGCAT ATGCA^T
4268 ATGCAT
PflMI 2495 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2530 CCAGGGTTTGG
PpuMI 4 AGGTCCT [GA]G^G[AT]CC[CT]
601 AGGACCC
PshBI 2723 ATTAAT AT^TAAT
4762 ATTAAT
Van91I 2495 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2530 CCAGGGTTTGG
XcmI 265 CCATGGCCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
699 CCAGACCAGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanII BbsI BbvI
BciVI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspHI
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HgaI HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI