##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AclI AgeI AhdI Aor51HI
AscI Asp718I AspEI AspI AviII
BbrPI BcgI BciVI BsaAI BsiWI
BspDI BssHII BssSI Bst1107I BstZ17I
ClaI CpoI DrdI Eam1105I Eco47III
EcoRV FseI FspI HpaI KpnI
MluI NaeI NdeI NgoMIV NotI
NruI PacI PflFI PinAI PmaCI
PmlI Psp1406I RsrII SbfI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
Tth111I
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 4716 GACGTC GACGT^C
AccIII 94 TCCGGA T^CCGGA
AflII 3921 CTTAAG C^TTAAG
Alw44I 2572 GTGCAC G^TGCAC
AocI 2711 CCTCAGG CC^T[ACGT]AGG
ApaI 137 GGGCCC GGGCC^C
ApaLI 2572 GTGCAC G^TGCAC
Asp700I 3068 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 5766 CCTAGG C^CTAGG
BamHI 2707 GGATCC G^GATCC
BfrI 3921 CTTAAG C^TTAAG
BglI 188 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5766 CCTAGG C^CTAGG
BlpI 839 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 839 GCTCAGC GC^T[ACGT]AGC
BsaWI 94 TCCGGA [AT]^CCGG[AT]
BseAI 94 TCCGGA T^CCGGA
BspEI 94 TCCGGA T^CCGGA
BstBI 2256 TTCGAA TT^CGAA
BstXI 5750 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2711 CCTCAGG CC^T[ACGT]AGG
CelII 839 GCTCAGC GC^T[ACGT]AGC
EagI 2701 CGGCCG C^GGCCG
EclXI 2701 CGGCCG C^GGCCG
Eco52I 2701 CGGCCG C^GGCCG
Eco81I 2711 CCTCAGG CC^T[ACGT]AGG
EcoRI 2460 GAATTC G^AATTC
KspI 68 CCGCGG CCGC^GG
MfeI 480 CAATTG C^AATTG
MroI 94 TCCGGA T^CCGGA
MunI 480 CAATTG C^AATTG
NcoI 189 CCATGG C^CATGG
NspV 2256 TTCGAA TT^CGAA
PaeR7I 1181 CTCGAG C^TCGAG
PmeI 1678 GTTTAAAC GTTT^AAAC
PshAI 5239 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4682 CGATCG CGAT^CG
SacII 68 CCGCGG CCGC^GG
SalI 3444 GTCGAC G^TCGAC
ScaI 3520 AGTACT AGT^ACT
SexAI 2490 ACCAGGT A^CC[AT]GGT
SfuI 2256 TTCGAA TT^CGAA
SmaI 23 CCCGGG CCC^GGG
SwaI 5664 ATTTAAAT ATTT^AAAT
XhoI 1181 CTCGAG C^TCGAG
XmaCI 23 CCCGGG C^CCGGG
XmaI 23 CCCGGG C^CCGGG
XmnI 3068 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1299 GTAGAC GT^[AC][GT]AC
3444 GTCGAC
AseI 3297 ATTAAT AT^TAAT
5104 ATTAAT
AsnI 3297 ATTAAT AT^TAAT
5104 ATTAAT
BbsI 1825 GAAGAC GAAGAC(2/6)
2877 GTCTTC
BglII 1268 AGATCT A^GATCT
2076 AGATCT
BpuAI 1825 GAAGAC GAAGAC(2/6)
2877 GTCTTC
BsaBI 317 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2651 GATAAAGATC
BsiEI 2701 CGGCCG CG[GA][CT]^CG
4682 CGATCG
BsmBI 63 CGTCTC CGTCTC(1/5)
4507 GAGACG
Bsp1407I 459 TGTACA T^GTACA
1076 TGTACA
BspHI 2659 TCATGA T^CATGA
4503 TCATGA
BspLU11I 456 ACATGT A^CATGT
4874 ACATGT
BspMI 781 ACCTGC ACCTGC(4/8)
5075 ACCTGC
BsrBI 144 GAGCGG CCGCTC(-3/-3)
1950 GAGCGG
BsrFI 2034 GCCGGT [GA]^CCGG[CT]
2289 ACCGGC
BsrGI 459 TGTACA T^GTACA
1076 TGTACA
BstEII 964 GGTGACC G^GT[ACGT]ACC
5514 GGTAACC
BstPI 964 GGTGACC G^GT[ACGT]ACC
5514 GGTAACC
Cfr10I 2034 GCCGGT [GA]^CCGG[CT]
2289 ACCGGC
EcoO65I 964 GGTGACC G^GT[ACGT]ACC
5514 GGTAACC
HgaI 62 GCGTC GACGC(5/10)
2937 GCGTC
HindIII 594 AAGCTT A^AGCTT
4990 AAGCTT
MamI 317 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2651 GATAAAGATC
NheI 218 GCTAGC G^CTAGC
3091 GCTAGC
PshBI 3297 ATTAAT AT^TAAT
5104 ATTAAT
RcaI 2659 TCATGA T^CATGA
4503 TCATGA
SacI 1385 GAGCTC GAGCT^C
2087 GAGCTC
SapI 1309 GAAGAGC GCTCTTC(1/4)
5373 GAAGAGC
SspBI 459 TGTACA T^GTACA
1076 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvCI BbvI BclI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII